Evaluation of microRNA‑486‑3p Molecular Signature in Patients with Autism Spectrum Disorder

Popov, Nikolay T.; Minkov, Ivan; Petrova, N. B.; Andreenko, Emilia; Stoyanova, Vili K.; Ivanov, Hristo; Vachev, Tihomir

doi:10.7546/crabs.2021.06.07

Cited by 1 publication

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are several limitations that need to be taken seriously both in interpreting the results from this systematic review and in planning for future research. The exact number of ASD participants from all included studies was difficult to ascertain as certain studies were not explicit in descriptions of study populations, and there were occasions where it was difficult to exclude some study population overlap [ 77 , 104 , 178 ]. The use of external datasets and biobank sample resources also made this challenging, with some instances where the same Gene Expression Omnibus (GEO) dataset was used (for example GSE18123 in three studies) [ 84 , 88 , 94 ].…”

Section: Discussionmentioning

confidence: 99%

“…For example, the use of an EDTA anticoagulant appears to influence specific miRNA expression, particularly after longer EDTA exposure times [ 196 ]. In our systematic review, EDTA blood tubes were used in several studies [ 73 , 75 , 76 , 89 , 90 , 93 , 101 , 105 ] with only a few studies using PAXgene blood RNA tubes [ 94 , 102 , 104 , 110 ] and many studies omitted details about blood sample collection, including anticoagulant exposure timings. Quantity and quality of centrifuging in blood has also been shown to alter the proportion of intra and extracellular components that may demonstrate different miRNA expression properties [ 197 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A systematic review of non-coding RNA genes with differential expression profiles associated with autism spectrum disorders

et al. 2023

View full text Add to dashboard Cite

Aims To identify differential expression of shorter non-coding RNA (ncRNA) genes associated with autism spectrum disorders (ASD). Background ncRNA are functional molecules that derive from non-translated DNA sequence. The HUGO Gene Nomenclature Committee (HGNC) have approved ncRNA gene classes with alignment to the reference human genome. One subset is microRNA (miRNA), which are highly conserved, short RNA molecules that regulate gene expression by direct post-transcriptional repression of messenger RNA. Several miRNA genes are implicated in the development and regulation of the nervous system. Expression of miRNA genes in ASD cohorts have been examined by multiple research groups. Other shorter classes of ncRNA have been examined less. A comprehensive systematic review examining expression of shorter ncRNA gene classes in ASD is timely to inform the direction of research. Methods We extracted data from studies examining ncRNA gene expression in ASD compared with non-ASD controls. We included studies on miRNA, piwi-interacting RNA (piRNA), small NF90 (ILF3) associated RNA (snaR), small nuclear RNA (snRNA), small nucleolar RNA (snoRNA), transfer RNA (tRNA), vault RNA (vtRNA) and Y RNA. The following electronic databases were searched: Cochrane Library, EMBASE, PubMed, Web of Science, PsycINFO, ERIC, AMED and CINAHL for papers published from January 2000 to May 2022. Studies were screened by two independent investigators with a third resolving discrepancies. Data was extracted from eligible papers. Results Forty-eight eligible studies were included in our systematic review with the majority examining miRNA gene expression alone. Sixty-four miRNA genes had differential expression in ASD compared to controls as reported in two or more studies, but often in opposing directions. Four miRNA genes had differential expression in the same direction in the same tissue type in at least 3 separate studies. Increased expression was reported in miR-106b-5p, miR-155-5p and miR-146a-5p in blood, post-mortem brain, and across several tissue types, respectively. Decreased expression was reported in miR-328-3p in bloods samples. Seven studies examined differential expression from other classes of ncRNA, including piRNA, snRNA, snoRNA and Y RNA. No individual ncRNA genes were reported in more than one study. Six studies reported differentially expressed snoRNA genes in ASD. A meta-analysis was not possible because of inconsistent methodologies, disparate tissue types examined, and varying forms of data presented. Conclusion There is limited but promising evidence associating the expression of certain miRNA genes and ASD, although the studies are of variable methodological quality and the results are largely inconsistent. There is emerging evidence associating differential expression of snoRNA genes in ASD. It is not currently possible to say whether the reports of differential expression in ncRNA may relate to ASD aetiology, a response to shared environmental factors linked to ASD such as sleep and nutrition, other molecular functions, human diversity, or chance findings. To improve our understanding of any potential association, we recommend improved and standardised methodologies and reporting of raw data. Further high-quality research is required to shine a light on possible associations, which may yet yield important information.

show abstract