Evaluation of milk replacer supplemented with lysine and methionine in combination with glutamate and glutamine in dairy calves

Silva, Jackeline Thais da; Manzoni, T.; Rocha, Nathália Brito; Santos, Glauber dos; Miqueo, Evangelina; Slanzon, Giovana Simão; Bittar, Carla Maris Machado

doi:10.1080/09712119.2018.1436549

Cited by 9 publications

(9 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in the present study, other studies with dairy calves reported an increase in the concentration of BHBA before weaning, with a marked increase after weaning [ 41 , 42 ]. Previous work by our laboratory evaluating supplementation with Lys and Met, in combination with glutamate and glutamine, for calves fed with MR, showed similar results [ 17 ].…”

Section: Discussionsupporting

confidence: 55%

“…However, in a series of studies by Hill et al [ 12 ], AA supplementation had no effect on starter concentrate intake when Met and Lys were included in a proportion of 3.25 (study 1), but it had a quadratic effect when Met inclusion was increased (0.64%, 0.68% and 0.72%, study 2). Silva et al [ 17 ] also found no effect on SC intake when Lys and Met were supplemented in combination with glutamate and glutamine for calves pre and post-weaning. In the present study, after weaning, the SC intake increased and quickly reached the expected values; however, the average intake was still lower for calves supplemented through SC, suggesting a different effect than that related to supplementation level.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the recommendation of 17 g/d Lys and 5.3 g/d Met for calves under 5 weeks of age has proved to be adequate to meet the requirements of calves in the study by Hill et al [ 12 ], supplementing MR containing 24% to 28% CP and 17% fat. Depending on the supplemented AA, there may be an increase in the average daily gain (ADG) [ 12 , 15 ] and positive or no response on growth [ 4 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lysine and Methionine Supplementation for Dairy Calves Is More Accurate through the Liquid than the Solid Diet

Silva

Miqueo

Torrezan

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

This study aimed to evaluate the performance and metabolic changes in dairy calves supplemented with lysine and methionine in milk replacer (MR) or starter concentrate (SC). Male Holstein calves (n = 45) were blocked and distributed in Control without supplementation (1) and; Lysine and Methionine supplementation to achieve an intake of 17 and 5.3 g/d in the SC (2) and to achieve of 17 and 5.3 g/d in the MR (3). MR was fed (6 L/d) until the 8th week of life when weaning occurred. Calves were followed until the 10th week of age. Feed intake was measured daily. Weight and body measurements were registered weekly. Blood samples were collected biweekly to evaluate the intermediate metabolism. The AA supplementation resulted in lower body weight at weaning and week 10. Calves fed SC Lys:Met had lower SC intake and lower total feed intake at weaning when compared to control. Calves fed control had higher heart girth, hip-width, and plasma glucose concentration. The supplementation with Lys and Met did not benefit dairy calves’ performance nor metabolism in this study. Supplementation through the MR was more efficient than SC to result in adequate daily intakes of AA. Further studies are needed to understand the negative effects of AA on calf starter intake.

show abstract

Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lysine and Methionine Supplementation for Dairy Calves Is More Accurate through the Liquid than the Solid Diet

Silva

Miqueo

Torrezan

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

show abstract

“…To improve calves’ weight gain ( WG ), several approaches are used such as food supplementation and restriction of milk supply during calf development. Methionine supplementation is an alternative that may have additive effects on protein metabolism, resulting in better growth rates ( Silva et al, 2018 ). However, since the dl -methionine is degraded in rumen, the alternative is to supplement with 2-hydroxy-4-(methylthio)butanoic acid ( HMTBa ), which is converted to methionine in various tissues ( Dibner and Knight, 1984 ).…”

Section: Introductionmentioning

confidence: 99%

Performance and serum parameters of calves (Bos taurus) subject to milk restriction associated with supplementation with 2-hydroxy-4-(methylthio)butanoic acid

Brito

França

Pansani

et al. 2021

Journal of Animal Science

View full text Add to dashboard Cite

Our aim, with this study was to evaluate the consumption, performance, quantitative characteristics of carcasses, biochemical profile, plasma levels of ghrelin and leptin, expression of the receptor for ghrelin (GHS-R1a) in the hypothalamus and duodenum, and the number of goblet cells in the duodenum of calves subjected to milk volume restriction and supplemented with 2-hydroxy-4-(methylthio)butanoate acid (HMTBa). We used 21 Holstein-mixed breed calves, aged between 3 and 15 days with an average weight of 36.8 kg, and housed in pens with troughs for hay, concentrate, and water. The study included two consecutive experimental periods (P1 and P2) of 21 days each, with seven days of adaptation to the diet and facilities. The calves were distributed in a completely randomized design in three treatments with seven repetitions. 1 - Control: 6 L of milk/day during P1 and 6 L of milk/day during P2; 2 – RES (milk restriction): 3 L of milk/day during P1 and 6 L of milk/day during P2; 3 - RES + HMTBa: 3 L of milk/day during P1 and 6 L of milk/day during P2 + 3.3 g of HMTBa/day in both periods. HMTBa was supplied in milk, and the amount of concentrated ration and hay provided and leftovers were recorded daily to estimate dry matter (DM) and crude protein (CP) consumption. Mean daily weight gain (DWG), final weight (FW), and feed conversion (FC) were obtained at the beginning and at the end of each 21-day period. Plasma concentrations of ghrelin and leptin, triglycerides, total protein, urea, lactate, creatinine, alkaline phosphatase, and cholesterol were measured for P1 and P2 at the end of each 21-day period. At the end of P2 animals were slaughtered; sections of the duodenum were collected to evaluate the expression of GHS-R1a and quantity of goblet cells; hypothalamus was used to evaluate the expression of GHS-R1a; rumen was used to evaluate the thickness of epithelium, keratin, density, and height and width of ruminal papillae. In P1, total DM consumption, FW, DWG, glucose and triglycerides were lower in the RES and RES+HMTBa groups (P < 0.001). In P2, there was an improvement in the FC of the RES+HMTBa group (compared to Control and RES) and a lower urea concentration in the RES group (compared to Control and RES+HMTBa) (P < 0.001). No differences were observed among groups regarding hormonal concentrations, histological parameters, and GHS-R1a expression in the duodenum and hypothalamus. Therefore, milk restriction combined with HMTBa supplementation promoted greater compensatory gain by a mechanism independent of changes in GHS-R1a expression and hormone levels of ghrelin and leptin.

show abstract

“…Calves of 3 days of age can tolerate well dietary supplementation of milk replacer containing 60% soy protein concentrate with 1% glutamine for 4 weeks as no differences were observed in final BW, average daily gain (ADG), dry matter (DM) intake and gain to feed in comparison to the group not supplemented with glutamine (Drackley et al., ). Weaned calves (8w of age) receiving a daily intake of 0.67 g/d of glutamine and 0.67 g/d of glutamate for 2 weeks showed no adverse effects on performance, faecal scores or metabolism (Da Silva et al., ). In a study by Plaizier et al.…”

Section: Assessmentmentioning

confidence: 99%

Safety and efficacy of l‐glutamine produced using Corynebacterium glutamicum NITE BP‐02524 for all animal species

Additives¹,

Bampidis²,

Azimonti³

et al. 2020

EFS2

View full text Add to dashboard Cite

Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of l‐glutamine (≥ 98.0%) produced by fermentation using a genetically modified strain of Corynebacterium glutamicum (NITE BP‐02524). It is intended to be used in feed for all animal species and categories as nutritional additive (amino acid) and as sensory additive (flavouring compound). Viable cells of the production strain and its recombinant DNA were not detected in the additive. l‐Glutamine manufactured by fermentation using C. glutamicum NITE BP‐02524 does not give rise to any safety concern with regard to the genetic modification of the production strain. The use of l‐glutamine produced by fermentation using C. glutamicum NITE BP‐02524 in animal nutrition is considered safe for all animal species when applied as a nutritional additive to achieve an adequate amino acid profile in feed and to overcome potential glutamine shortages during critical periods of life. The proposed use level (25 mg/kg feed) when used as sensory additive (flavouring compound) is safe for all animal species. The uses of l‐glutamine produced using C. glutamicum NITE BP‐02524 as nutritional additive or as flavouring compound are considered safe for the consumer. l‐Glutamine produced using C. glutamicum NITE BP‐02524 is not toxic by inhalation, is non‐irritant to skin and eyes and is not a skin sensitiser. l‐Glutamine produced using C. glutamicum NITE BP‐02524 is considered safe for the environment. l‐glutamine is a non‐essential amino acid and it plays a physiological role as such. Recent evidence shows that glutamine may act as conditionally essential amino acid mainly in growing animals and has some specific effects e.g. in improving intestinal development and immune response. This amino acid produced by fermentation using C. glutamicum NITE BP‐02524 is regarded as an efficacious source of glutamine for all animal species. For supplemental l‐glutamine to be as efficacious in ruminants as in non‐ruminants, it would require protection against degradation in the rumen. The use of l‐glutamine as sensory additive at 25 mg/kg feed is considered efficacious.

show abstract

Evaluation of milk replacer supplemented with lysine and methionine in combination with glutamate and glutamine in dairy calves

Cited by 9 publications

References 30 publications

Lysine and Methionine Supplementation for Dairy Calves Is More Accurate through the Liquid than the Solid Diet

Lysine and Methionine Supplementation for Dairy Calves Is More Accurate through the Liquid than the Solid Diet

Performance and serum parameters of calves (Bos taurus) subject to milk restriction associated with supplementation with 2-hydroxy-4-(methylthio)butanoic acid

Safety and efficacy of l‐glutamine produced using Corynebacterium glutamicum NITE BP‐02524 for all animal species

Contact Info

Product

Resources

About