Evaluation of MIRU-VNTR for typing of Mycobacterium bovis isolated from Sika deer in Northeast China

Abstract

BackgroundBovine tuberculosis has led to serious economic losses for Sika Deer producers in China. Strategies for controlling the spread of Mycobacterium bovis are often hampered by a lack of epidemiological data. Specifically, tracing infections requires the ability to trace back infections, which, in turn, requires the ability to determine isolates with a common source. This study was planned to assess the discriminatory power of each mycobacterial interspersed repetitive unit (MIRU)-variable number tandem repeats (VNTR) locus and evaluate the most appropriate combination of MIRU-VNTR loci for molecular epidemiological studies on Sika Deer in China.ResultsThe discriminatory power of MIRU-VNTR typing based on 22 known loci (12 MIRUs, 2 ETRs, 4 QUBs, and 4 Mtubs) were assessed in 96 Mycobacterium bovis strains collected sequentially from Sika Deer at a slaughterhouse in northeastern China. We defined four loci (MIRU4, ETRA, QUB11b, and Mtub4) as highly discriminative, eight loci (MIRU2, MIRU23, MIRU27, MIRU31, MIRU39, MIRU40, QUB26, and Mtub21) as moderately discriminative, and three loci (MIRU16, Mtub30, and Mtub34) as poorly discriminative. The final locus showed no polymorphism between strains. MIRU-VNTR typing as a whole was highly discriminative, with an overall allelic diversity of 0.897. Of the loci tested, the four highly discriminative loci and eight moderately discriminative loci proved to be most appropriate for first line typing of M. bovis from Sika Deer, with the same resolving ability as all 22 loci (H = 0.897).ConclusionsMIRU-VNTR typing is quick and effective for typing bovine tuberculosis isolates from Sika Deer in China.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-015-0402-0) contains supplementary material, which is available to authorized users.

Dynamic network biomarker indicates pulmonary metastasis at the tipping point of hepatocellular carcinoma

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Typing of Mycobacterium bovis isolates from cattle using MIRU-VNTR analysis

“…Genotyping studies are not only effective for regional backwards tracing, but also for the global comparison of tuberculosis isolates (Yang et al 2015).…”

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Prevención y diagnóstico veterinario de la tuberculosis bovina. Una revisión de las tendencias globales

“…Los beneficios de su uso están asociados con la posibilidad de distinguir cepas relacionadas epidemiológicamente, comparar los resultados con otros laboratorios de países con escenarios epidemiológicos diferentes y no requerir grandes cantidades de ADN. Su empleo en ciervos permitió demostrar que los loci MIRU4, ETRA, QUB11b y Mtub4 muestran elevada capacidad discriminatoria para diferenciar las distintas micobacterias que afectan a esta especie silvestre [78]. Esta técnica ha sido de mucha utilidad para las zonas ganaderas que comparten fronteras comunes.…”