Evaluation of Mitochondrial Function and Morphology in Drosophila

Tang, Yinglu; Tahmasebinia, Foozhan; Wu, Zhihao

doi:10.1007/978-1-0716-1495-2_19

Cited by 4 publications

(1 citation statement)

References 28 publications

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“…Considerable evidence in recent studies showed that the progressive degeneration of dopaminergic (DA) neurons in the substantia nigra (SN) and the parenchyma (SNpc) was the main cause of the motor symptoms 2 , and the pathological hallmarks of PD are the deposition of Lewy bodies (LBs), which are mainly composed of misfolding α-synuclein (α-Syn) 3 . For this reason, several trans-genetic Drosophila melanogaster, such as E46K mutation of SNCA gene, have been utilized as animal models to explore the pathological mechanism of PD induced by single gene mutation [4][5][6] .…”

Section: Introductionmentioning

confidence: 99%

Deep Learning Behavioral Phenotyping System in the Diagnosis of Parkinson’s Disease with Drosophila melanogaster

Dong,

Burch,

Huang

2024

Preprint

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Drosophila Melanogasteris widely used as animal models for Parkinson’s disease (PD) research. Because of the complexity of MoCap and quantitative assessment amongDrosophila Melanogaster, however, there is a technical issue that identify PD symptoms within drosophila based on objective spontaneous behavioral characteristics. Here, we developed a deep learning framework generated from kinematic features of body posture and motion between wildtype and SNCAE46Kmutant drosophila genetically modeled □-Syn, supporting clustering and classification of PD individuals. We record locomotor activity in a 3D-printed trap, and utilize the pre-analysis pose estimation software DeepLabCut (DLC) to calculate and generate numerical data representing the motion speed, tremor frequency, and limb motion ofDrosophila Melanogaster. By plugging these data as the input, the diagnosis result (1/0) representing PD or WT as the output. Our result provides a toolbox which would be valuable in the investigation of PD progressing and pharmacotherapeutic drug development.

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