Evaluation of modified Wright-staining of urine sediment as a method for accurate detection of bacteriuria in dogs

Swenson, Cheryl L.; Boisvert, Agatha M.; Kruger, John M.; Gibbons-Burgener, Suzanne

doi:10.2460/javma.2004.224.1282

Cited by 59 publications

(76 citation statements)

References 16 publications

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“…Often the constituents will wash off during the staining process because of the low-protein nature of the specimen. Serum-coated slides should be used to "glue" the constituents in the sediment to the surface of the slide (Swenson et al, 2004).…”

Section: Sediment Preparationmentioning

confidence: 99%

Microscopic Examination of the Urinary Sediment

Meyer¹

2016

Canine and Feline Cytology

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Section: Sediment Preparationmentioning

confidence: 99%

Microscopic Examination of the Urinary Sediment

Meyer¹

2016

Canine and Feline Cytology

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“…Τα φυσιολογικά ούρα είναι άσηπτα και μπορεί να περιέχουν μικρό αριθμό κυττάρων (λευκά και ερυθρά αιμοσφαίρια, επιθηλιακά κύτταρα), κρυστάλλους και σποραδικούς κυλίνδρους, λιποσφαίρια και σπερματοζωάρια στα αρσενικά ζώα. Αντίθετα, μεγάλοι αριθμοί κυττάρων ή κυλίνδρων, η παρατήρηση ασυνήθιστων τύπων κρυστάλλων και η παρουσία νεοπλασματικών κυττάρων, παρασίτων και μικροοργανισμών (βακτήρια, μύκητες), αποτελούν παθολογικά ευρήματα που Σ τη μικροσκοπική εξέταση του ιζήματος σε συνδυα-σμό με τη φυσική και βιοχημική ανάλυση των ούρων μπορεί να βασιστεί η διάγνωση, όχι μόνο των παθήσεων του ουροποιητικού συστήματος, αλλά και πολλών μετα-βολικών νοσημάτων (Swenson et al 2004, Chew et al 2011, Fry 2011. Επιπλέον, τα ευρήματα της εξέτασης του ιζήματος (π.χ.…”

Section: περίληψηunclassified

“…Για την παρασκευή βαμμένου, μονιμοποιημένου με μεθανό-λη, επιχρίσματος, μεταφέρουμε μια σταγόνα ούρων από το ίζημα σε αντικειμενοφόρο πλάκα και, αφού στεγνώσει, ακολουθεί η μονιμοποίηση και η χρώση με τις συνήθεις κυτταρολογικές χρώσεις (π.χ. DiffQuik) (Swenson et al 2004). …”

Section: παρασκευη και μικροσκοπηση του ιζηματος ουρωνunclassified

“…Stevens 1999, Christopher 2003, Reine and Langston 2005. Η εξέταση βαμμένων επιχρισμάτων ιζήματος παρουσιάζει μεγαλύτερη αξιοπιστία σε σχέση με αυτή του νωπού ιζήματος για την επιβεβαίωση της βακτηριουρίας (Swenson et al 2004(Swenson et al , ζinkl 2008. Σημειώνεται οτι η πολύωρη έκθεση των ούρων σε θερμοκρασία περιβάλλοντος μπορεί να οδηγήσει σε βακτηριακή υπερανάπτυξη (Chew et al 2011).…”

Section: μικροοργανισμοίunclassified

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Urine sediment evaluation in the dog and cat

Alatzas¹,

Mylonakis²,

Polizopoulou³

et al. 2017

J Hellenic Vet Med Soc

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Microscopic examination of the urine sediment is an integral part of urinalysis and a useful, cost-effective diagnostic tool in the everyday practice. Diagnostic investigation of urinary diseases and several systemic metabolic diseases make the microscopic evaluation of urine sediment a priority along with the physical and biochemical examination of urine. The different methods of urine collection (midstream voiding, catheterization or percutaneous cystocentesis) have advantages and disadvantages and the clinician has to decide on the best fitted method of urine collection, according to the medical background of the animal and the case-specific objectives of urinalysis. Proper handling and timely analysis of urine sample are essential for a valid microscopic evaluation. The microscopic examination of urinary sediment is usually conducted in stained or unstained “wet-mounted” preparations; occasionally, air-dried Giemsa-stained sediment smears are examined.Normal urine is sterile and may contain small numbers of cells (white and red blood cells, epithelial cells), few crystals, occasional casts, fat droplets and spermatozoa (male animals). In contrast, large numbers of cells or casts, presence of unusual types of crystals, neoplastic cells, parasites and microorganisms comprise abnormal findings, necessitating a more specialized diagnostic approach. This review focuses on the technical aspects pertaining to the proper sediment microscopic examination, the normally expected elements of sediment and the clinically relevant interpretation of abnormal findings.

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“…Furthermore, studies assessing the validity of microscopy show wide variation in sensitivity (60–100 %) and specificity (49–100 %) to predict significant bacteriuria in humans [26, 27] and in companion animals [28–29]. There are no studies comparing microscopy with more recent diagnostic tools such as point-of-care culture and susceptibility testing in human and veterinary primary care practice.…”

Section: Introductionmentioning

confidence: 99%

Exploring the feasibility and synergistic value of the One Health approach in clinical research: protocol for a prospective observational study of diagnostic pathways in human and canine patients with suspected urinary tract infection

Córdoba

Sørensen

Holm

et al. 2015

Pilot Feasibility Stud

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BackgroundThe One Health approach is emerging in response to the development of bacterial resistance. To the best of our knowledge, the possibility to use this approach in a clinical context has not yet been explored. Thus, in this paper, we report the procedures to implement a prospective observational study of diagnostic pathways in human and canine patients with suspected urinary tract infection as a means to assess the feasibility and synergistic value of setting up One Health clinical research projects and interventions.Methods/designA prospective observational study will compare different diagnostic pathways (i.e., 16 possible combinations of diagnostic tools) to gold standard in human and veterinary primary care practice in Denmark. Fifty primary care practices and 100 veterinary clinics will each consecutively include 20 human patients or 8–10 dogs, respectively. Data will be collected at practice and patient level comprising (a) information about the organization of the practice and access to different diagnostic tools, (b) information about clinical history, diagnostic path and treatment during the index consultation, (c) information about severity of symptoms during the 7–10 days following inclusion, and (d) urine culture (type of microorganism and susceptibility test). The feasibility and synergistic value of conducting future research, and/or designing common interventions, will be assessed by evaluating the comparability of human primary care and veterinary primary care with respect to study implementation and study results.DiscussionResults from this study will give an insight into the feasibility and synergistic value of setting-up One Health research projects in a clinical context. This is crucial if we are to embrace the One Health approach, as a legitimate strategy to implement common interventions aimed at influencing the diagnostic process in human and canine patients in order to decrease inappropriate use of antibiotics.Trial registrationThe study in humans has been registered in ClinicalTrials.gov NCT02249273.Electronic supplementary materialThe online version of this article (doi:10.1186/s40814-015-0036-9) contains supplementary material, which is available to authorized users.

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Evaluation of modified Wright-staining of urine sediment as a method for accurate detection of bacteriuria in dogs

Cited by 59 publications

References 16 publications

Microscopic Examination of the Urinary Sediment

Microscopic Examination of the Urinary Sediment

Urine sediment evaluation in the dog and cat

Exploring the feasibility and synergistic value of the One Health approach in clinical research: protocol for a prospective observational study of diagnostic pathways in human and canine patients with suspected urinary tract infection

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