Evaluation of molecular mechanisms of heparin-induced angiogenesis, in human umbilical vein endothelial cells

Fazil, Sadia; Shah, Humaira; Noreen, Mamoona; Yar, Muhammad; Khan, Ather Farooq; Safi, Sher Zaman; Alomar, Suliman Yousef; Alkhuriji, Afrah Fahad; Alharbi, Hanan; Afzal, Muhammad Sohail

doi:10.1016/j.jksus.2021.101534

Cited by 5 publications

(3 citation statements)

References 37 publications

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“… 23,24 The C–O–S stretching vibration absorption peak of the sulfuric acid group on amino hexose appeared around 830 cm −1 , the strong absorption peak of O–S stretching vibration appeared around 1240 cm −1 , and the C O stretching vibration peak of the sulfuric acid group in the carboxyl group appeared around 1621 cm −1 was also found in both, all of these indicated that the main structure of the OL obtained after oxidation did not change significantly. It has been known that the reason for the conspicuous anticoagulant activity and cytocompatibility of LMWH is the presence of a reactive pentose structure in its molecular chain, 25 they can bind to antithrombin III (AT III), change the conformation of the antithrombin molecules, and inhibit the activity of coagulation factors IIa, Xa and IXa, exhibiting strong anticoagulant properties. The similarity of molecular structure between OL and LMWH is of great significance to retain the anticoagulant activity of OL.…”

Section: Resultsmentioning

confidence: 99%

Crosslinking of dialdehyde heparin: a new strategy for improving the anticoagulant properties of porcine acellular dermal matrix

Feng

Dan

2022

RSC Adv.

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Section: Resultsmentioning

confidence: 99%

Crosslinking of dialdehyde heparin: a new strategy for improving the anticoagulant properties of porcine acellular dermal matrix

Feng

Dan

2022

RSC Adv.

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“…Heparin, in combination with other biomaterials, increases angiogenesis (new vessel formation) and thus plays a role in the wound healing process. In our previous study, we also demonstrated the involvement of the VEGF gene in biomaterial-induced angiogenesis [ 46 ]. Keeping in view the pro-angiogenic and anti-microbial potential of heparin along with many other known therapeutic properties, we investigated the heparin-loaded alginate dressings.…”

Section: Discussionmentioning

confidence: 99%

Heparin-Loaded Alginate Hydrogels: Characterization and Molecular Mechanisms of Their Angiogenic and Anti-Microbial Potential

et al. 2022

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Background: Chronic wounds continue to be a global concern that demands substantial resources from the healthcare system. The process of cutaneous wound healing is complex, involving inflammation, blood clotting, angiogenesis, migration and remodeling. In the present study, commercially available alginate wound dressings were loaded with heparin. The purpose of the study was to enhance the angiogenic potential of alginate wound dressings and analyze the antibacterial activity, biocompatibility and other relevant properties. We also aimed to conduct some molecular and gene expression studies to elaborate on the mechanisms through which heparin induces angiogenesis. Methods: The physical properties of the hydrogels were evaluated by Fourier transform infrared spectroscopy (FTIR). Swelling ability was measured by soaking hydrogels in the Phosphate buffer at 37 °C, and cell studies were conducted to evaluate the cytotoxicity and biocompatibility of hydrogels in NIH3T3 (fibroblasts). Real-time PCR was conducted to check the molecular mechanisms of heparin/alginate-induced angiogenesis. The physical properties of the hydrogels were evaluated by Fourier transform infrared spectroscopy (FTIR). Results: FTIR confirmed the formation of heparin-loaded alginate wound dressing and the compatibility of both heparin and alginate. Among all, 10 µg/mL concentration of heparin showed the best antibacterial activity against E. coli. The swelling was considerably increased up to 1500% within 1 h. Alamar Blue assay revealed no cytotoxic effect on NIH3T3. Heparin showed good anti-microbial properties and inhibited the growth of E. coli in zones with a diameter of 18 mm. The expression analysis suggested that heparin probably exerts its pro-angiogenetic effect through VEGF and cPGE. Conclusions: We report that heparin-loaded alginate dressings are not cytotoxic and offer increased angiogenic and anti-bacterial potential. The angiogenesis is apparently taken through the VEGF pathway.

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“…There were only a few endothelial cells attached to the G-PP after 24 h or 48 h with the poor morphological phenotype and the growth condition of the cells. The cells of OX-CO-PP, OX-SC-PP and OX-SH-PP were spindle-shaped after 24 h, and the number of cells significantly increased after 48 h with normal morphological phenotype and good growth condition, indicating the good cell compatibility and the enhanced growth of endothelial cells for OX-SH-PP [39,40].…”

Section: Cytotoxicity Of Ppsmentioning

confidence: 97%

Functional non-glutaraldehyde treated porcine pericardium for anti-coagulation, anti-calcification, and endothelial proliferation bioprosthetic heart valves

Chen

Kong

et al. 2022

J Leather Sci Eng

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In the last decade, the number of transcatheter heart valve replacement for severe heart valve disease has increased exponentially. Although the bioprosthetic artificial heart valve (BHV) has similar fluid dynamics performance to the original heart valve compared with mechanical heart valve so that there is no need to take long-term anticoagulant drugs to prevent thromboembolism, transcatheter BHV replacement are still at risk for thrombosis during the first few months according to the clinical data. However, the use of antithrombotic drugs can also increase the risk of bleeding. Therefore, it is particularly important to improve the anticoagulant properties for the BHV itself. In this work, a kind of non-glutaraldehyde cross-linked BHV material with excellent antithrombotic ability has been prepared from carboxylated oxazolidine treated porcine pericardium (consisting of collagen, elastin and glycoprotein) with the further graft of the anticoagulant heparin sodium via hydrophilic modified chitosan. Along with the similar mechanical properties and collagen stability comparable to the glutaraldehyde cross-linked porcine pericardium (PP), these functional non-glutaraldehyde cross-linked PPs exhibit better biocompatibility, promoted endothelial proliferation and superior anti-calcification ability. More importantly, excellent anticoagulant activity can be observed in the hematological experiments in vivo and in vitro. In summary, these excellent performances make these functional non-glutaraldehyde cross-linked PPs great potentialities in the BHV applications. Graphical abstract

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Evaluation of molecular mechanisms of heparin-induced angiogenesis, in human umbilical vein endothelial cells

Cited by 5 publications

References 37 publications

Crosslinking of dialdehyde heparin: a new strategy for improving the anticoagulant properties of porcine acellular dermal matrix

Crosslinking of dialdehyde heparin: a new strategy for improving the anticoagulant properties of porcine acellular dermal matrix

Heparin-Loaded Alginate Hydrogels: Characterization and Molecular Mechanisms of Their Angiogenic and Anti-Microbial Potential

Functional non-glutaraldehyde treated porcine pericardium for anti-coagulation, anti-calcification, and endothelial proliferation bioprosthetic heart valves

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