Evaluation of morphological and biochemical variations in pea under two widespread abiotic stresses

Farooq, Muhammad; Ahmad, Rafiq; Shahzad, Muhammad; Ahmad, Khalid; Sajjad, Yasar; Hassan, Amjad; Nazeer, Abdul; Shah, Mohammad Maroof; B., Kalsoom; Khan, Sabaz Ali

doi:10.21203/rs.3.rs-2994622/v1

Cited by 3 publications

(1 citation statement)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, salt stress significantly increased the contents of flavonoid and anthocyanin in all three cultivars. Similar results have been reported in Brassica napus (Kim et al, 2017) and pea (Farooq et al, 2021), which found that the contents of total flavonoid and phenolic compounds showed a significant increase to improve resistance in response to salt stress. In this study, JHR1 and JHB1 showed the higher increase of anthocyanin and flavonoid than YZ9102 under salt stress.…”

Section: Discussionsupporting

confidence: 89%

Physiological and biochemical mechanisms underlying the role of anthocyanin in acquired tolerance to salt stress in peanut (Arachis hypogaea L.)

Li,

Guo,

Sun

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Anthocyanin is an important pigment that prevents oxidative stress and mediates adaptation of plants to salt stress. Peanuts with dark red and black testa are rich in anthocyanin. However, correlation between salt tolerance and anthocyanin content in black and dark red testa peanuts is unknown. In this study, three peanut cultivars namely YZ9102 (pink testa), JHR1 (red testa) and JHB1 (black testa) were subjected to sodium chloride (NaCl) stress. The plant growth, ion uptake, anthocyanin accumulation, oxidation resistance and photosynthetic traits were comparatively analyzed. We observed that the plant height, leaf area and biomass under salt stress was highly inhibited in pink color testa (YZ9102) as compare to black color testa (JHB1). JHB1, a black testa colored peanut was identified as the most salt-tolerance cultivar, followed by red (JHR1) and pink(YZ9102). During salt stress, JHB1 exhibited significantly higher levels of anthocyanin and flavonoid accumulation compared to JHR1 and YZ9102, along with increased relative activities of antioxidant protection and photosynthetic efficiency. However, the K+/Na+ and Ca2+/Na+ were consistently decreased among three cultivars under salt stress, suggesting that the salt tolerance of black testa peanut may not be related to ion absorption. Therefore, we predicted that salt tolerance of JHB1 may be attributed to the accumulation of the anthocyanin and flavonoids, which activated antioxidant protection against the oxidative damage to maintain the higher photosynthetic efficiency and plant growth. These findings will be useful for improving salt tolerance of peanuts.

show abstract

Section: Discussionsupporting

confidence: 89%

Physiological and biochemical mechanisms underlying the role of anthocyanin in acquired tolerance to salt stress in peanut (Arachis hypogaea L.)

Li,

Guo,

Sun

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

Real-time expression and in silico characterization of pea genes involved in salt and water-deficit stress

Farooq,

Ahmad,

Shahzad

et al. 2023

Mol Biol Rep

Self Cite

View full text Add to dashboard Cite

BackgroundTo tolerate salt and water-de cit stress, the plant adapts to the adverse environment by regulating its metabolism and expressing certain stress-induced metabolic pathways. This research analyzed the relative expression of four pea genes (proC, PAL1, SOD, and POX) in three pea varieties (Climax, Green grass, and Meteor) under different levels of salt and water-de cit stress. Methods and ResultsResults from RT-qPCR analysis showed increased expression of proC, PAL1, and POX genes, while SOD gene expression decreased under both stresses. Climax exhibited superior stress tolerance with elevated expression of proC and PAL1, while Meteor showed better tolerance through increased POX expression. Phylogenetic analysis revealed common ancestry with other species like chickpea, red clover, mung bean, and barrel clover, suggesting about the cross relationship among these plant species. Conserved domain analysis of respective proteins revealed that these proteins contain PLNO 2688, PLN02457, Cu-Zn Superoxide dismutase, and secretory peroxidase conserved domains. Furthermore, protein family classi cation indicated that the oxidation-reduction process is the most common chemical process involved in these stresses given to peas which validate the relationship of these proteins. ConclusionsSalt and water-de cit stresses trigger distinct metabolic pathways, leading to the upregulation of speci c genes and the synthesis of corresponding proteins. These ndings further emphasize the conservation of stress-tolerance-related genes and proteins across various plant species. This knowledge enhances our understanding of plant adaptation to stress and offers opportunities for developing strategies to improve stress resilience in crops, thereby addressing global food security challenges.

show abstract

Influence of Super-Absorbent Polymer on Growth and Productivity of Green Bean under Drought Conditions

Alotaibi,

Alharbi,

Alsudays

et al. 2024

Agronomy

View full text Add to dashboard Cite

The water-retaining and yield-increasing capacity of super-absorbent polymer (SAP) are essential for soil remediation in arid and semi-arid areas. Water availability is an increasing challenge to plant development and crop yield. During the growing seasons in 2021 and 2022, the present study was conducted to evaluate the effect of the addition of different amounts of SAP on the development and yield of green beans (Phaseolus vulgaris L. cv Bronco) under varying water deficit stresses, compared with the control treatment without SAP and water deficit stress. The results demonstrated that a 50% reduction in water requirement (WR) resulted in significant decreases in leaf fresh weight, specific leaf area, leaf total chlorophyll content, pod number, leaf free water content, pod fresh weight per plant, and yield. Decreases were also found in pod total chlorophyll content, carotenoids, dry matter and total protein, leaf proline content, and crude fiber content. Additionally, leaf water saturation deficit was significantly increased under the stress compared with the full irrigation at 100% WR. However, irrigation at 75% WR increased pod contents of ascorbic acid, total sugars, and leaf bound water. The current study also indicated that addition of SAP significantly enhanced the above-mentioned growth characteristics under irrigation at 50% and 75% WR. Treatment with SAP at 3 g/plant was the most effective in mitigating the adverse effects of water deficiency, especially at the irrigation rate of 75% WR. Pearson’s correlation analysis showed significantly positive correlations between the growth parameters, as well as pod yield, under water stress and SAP. This study provides a promising strategy for green bean cultivation by adding SAP to soil to alleviate water shortage stress.

show abstract

Evaluation of morphological and biochemical variations in pea under two widespread abiotic stresses

Cited by 3 publications

References 88 publications

Physiological and biochemical mechanisms underlying the role of anthocyanin in acquired tolerance to salt stress in peanut (Arachis hypogaea L.)

Physiological and biochemical mechanisms underlying the role of anthocyanin in acquired tolerance to salt stress in peanut (Arachis hypogaea L.)

Real-time expression and in silico characterization of pea genes involved in salt and water-deficit stress

Influence of Super-Absorbent Polymer on Growth and Productivity of Green Bean under Drought Conditions

Contact Info

Product

Resources

About