Evaluation of mouthrinse and dentifrice regimens in an in situ erosion remineralisation model

Maggio, Brenda; Guibert, Rita G.; Mason, Stephen; Karwal, Ritu; Rees, Gareth D.; Kelly, Sue; Zero, Domenick T.

doi:10.1016/s0300-5712(11)70007-0

Cited by 22 publications

(23 citation statements)

References 31 publications

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“…Analogously to the previously shown antierosive properties of Sn/F-containing solutions [Ganss et al, 2008], there is some evidence that Sn-containing toothpastes might be more effective than conventional NaF toothpastes [Young et al, 2006;Hooper et al, 2007]. For a toothpaste with anti-erosion claim containing NaF and 5% KNO 3 , a significant potential to reharden eroded enamel surfaces has been shown [Zero et al, 2006;Hara et al, 2009b;Maggio et al, 2010], but studies quantifying erosive enamel loss have revealed conflicting results [Rees et al, 2007;Kato et al, 2010].…”

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confidence: 89%

Conventional and Anti-Erosion Fluoride Toothpastes: Effect on Enamel Erosion and Erosion-Abrasion

et al. 2011

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New toothpastes with anti-erosion claims are marketed, but little is known about their effectiveness. This study investigates these products in comparison with various conventional NaF toothpastes and tin-containing products with respect to their erosion protection/abrasion prevention properties. In experiment 1, samples were demineralised (10 days, 6 × 2 min/day; citric acid, pH 2.4), exposed to toothpaste slurries (2 × 2 min/day) and intermittently stored in a mineral salt solution. In experiment 2, samples were additionally brushed for 15 s during the slurry immersion time. Study products were 8 conventional NaF toothpastes (1,400–1,490 ppm F), 4 formulations with anti-erosion claims (2 F toothpastes: NaF + KNO₃ and NaF + hydroxyapatite; and 2 F-free toothpastes: zinc-carbonate-hydroxyapatite, and chitosan) and 2 Sn-containing products (toothpaste: 3,436 ppm Sn, 1,450 ppm F as SnF₂/NaF; gel: 970 ppm F, 3,030 ppm Sn as SnF₂). A mouth rinse (500 ppm F as AmF/NaF, 800 ppm Sn as SnCl₂) was the positive control. Tissue loss was quantified profilometrically. In experiment 1, most NaF toothpastes and 1 F-free formulation reduced tissue loss significantly (between 19 and 42%); the Sn-containing formulations were the most effective (toothpaste and gel 55 and 78% reduction, respectively). In experiment 2, only 4 NaF toothpastes revealed significant effects compared to the F-free control (reduction between 29 and 37%); the F-free special preparations and the Sn toothpaste had no significant effect. The Sn gel (reduction 75%) revealed the best result. Conventional NaF toothpastes reduced the erosive tissue loss, but had limited efficacy regarding the prevention of brushing abrasion. The special formulations were not superior, or were even less effective.

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confidence: 89%

Conventional and Anti-Erosion Fluoride Toothpastes: Effect on Enamel Erosion and Erosion-Abrasion

et al. 2011

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“…21,26 Similarly, fluoride has been added to mouthwashes formulations due to its effect in caries prevention 23 and in dental remineralization. 27 Based on this body of evidence, the aim of this study was to evaluate the effectiveness of a mouthwash formulation containing 0.075% CPC, 0.28% zinc lactate and 0.05% sodium fluoride (CPC+Zn+F), in reducing viable bacteria present in oral aerosol/splatter.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of a pre-procedural mouthwash in reducing bacteria in dental aerosols: randomized clinical trial

et al. 2017

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The aim of this randomized, single blinded clinical trial was to evaluate the effect of a pre-procedural mouthwash containing cetylpyridinium chloride (CPC), zinc lactate (Zn) and sodium fluoride (F) in the reduction of viable bacteria in oral aerosol after a dental prophylaxis with ultrasonic scaler. Sixty systemically healthy volunteers receiving dental prophylaxis were randomly assigned to one of the following experimental groups (15 per group): (i) rinsing with 0.075% CPC, 0.28% Zn and 0.05% F (CPC+Zn+F), (ii) water or (iii) 0.12% chlorhexidine digluconate (CHX), and (iv) no rinsing. Viable bacteria were collected from different locations in the dental office on enriched TSA plates and anaerobically incubated for 72 hours. The colonies were counted and species were then identified by Checkerboard DNA-DNA Hybridization. The total number of colony-forming units (CFUs) detected in the aerosols from volunteers who rinsed with CPC+Zn+F or CHX was statistically significantly (p<0.05) lower than of those subjects who did not rinse or who rinsed with water. When all locations were considered together, the aerosols from the CPC+Zn+F and CHX groups showed, respectively, 70% and 77% fewer CFUs than those from the No Rinsing group and 61% and 70% than those from the Water group. The mean proportions of bacterial species from the orange complex were statistically significantly (p<0.05) lower in aerosols from the CPC+Zn+F and CHX groups compared with the others two groups. In conclusion, the mouthwash containing CPC+Zn+F, is effective in reducing viable bacteria in oral aerosol after a dental prophylaxis with ultrasonic scaler.

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“…Recent studies have reported that fluoride prevents tissue loss from the partially demineralized dentin when subjected to acid attack under erosive conditions [Schlueter et al, 2007[Schlueter et al, , 2009Diamanti et al, 2010;Ganss et al, 2010]. This protective effect may depend on several factors, such as the type of fluoride compound [Wiegand et al, 2009;Ganss et al, 2010Ganss et al, , 2014, fluoride concentration [Diamanti et al, 2010], exposure time and exposure vehicle [Maggio et al, 2010;Magalhães et al, 2010Magalhães et al, , 2012. Even though the protective effect is thought to be derived from enhanced remineralization of dentin due to the formation of fluoridated hydroxyapatite, the protection of collagen matrix against enzymatic degradation may also contribute to the overall efficiency of fluoride ions, which has recently been shown as a potent inhibitor of the catalytic activities of recombinant MMPs [Kato et al, 2014].…”

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confidence: 99%

NaF Inhibits Matrix-Bound Cathepsin-Mediated Dentin Matrix Degradation

et al. 2016

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Matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs) degrade the collagen fibrils of demineralized dentin. Sodium fluoride (NaF) has previously been shown to inhibit recombinant MMP-2 and MMP-9. This study aimed to evaluate the efficacy of NaF on the inhibition of dentin-bound MMPs and CCs. Dentin beams were completely demineralized in 10% phosphoric acid. The baseline total MMP activity and dry masses were measured. Beams were assigned to test groups based on similar MMP activity and dry mass (n = 10/group), and incubated in artificial saliva (control) or artificial saliva with NaF containing 6-238 mM fluoride for 1, 7 and 21 days. The dry mass loss and MMP activities were reassessed at each time point. The proteolytic activity was screened by gelatin zymography. ICTP and CTX released to the incubation medium were analyzed as indices of MMP and cathepsin K activity, respectively. The beams were examined under scanning electron microscopy. All NaF doses reduced the dry mass loss after 21 days (p < 0.05). NaF inhibition of the total MMP activity ranged between 5 and 80%. In gelatin zymography, the bands of MMP-2 and MMP-9 became less prominent with increasing NaF levels. NaF did not decrease the released ICTP (p > 0.05). Less CTX release was detected with F ≥179 mM (p < 0.05). CaF₂-like minerals were observed on the beams. High levels of NaF may slow the degradation of the dentin matrix due to the inhibition of cathepsin K. Fluoride does not seem effective in the direct inhibition of proteolysis by dentin matrix-bound MMPs.

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Evaluation of mouthrinse and dentifrice regimens in an in situ erosion remineralisation model

Cited by 22 publications

References 31 publications

Conventional and Anti-Erosion Fluoride Toothpastes: Effect on Enamel Erosion and Erosion-Abrasion

Conventional and Anti-Erosion Fluoride Toothpastes: Effect on Enamel Erosion and Erosion-Abrasion

Effectiveness of a pre-procedural mouthwash in reducing bacteria in dental aerosols: randomized clinical trial

NaF Inhibits Matrix-Bound Cathepsin-Mediated Dentin Matrix Degradation

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