Evaluation of multifunctional activity of bioactive peptide fractions from the leaves of Nauclea diderrichii (De Wild. and T. Durand) Merrill and Ixora brachypoda DC

Ogbole, Omonike O.; Nkumah, Abraham O.; Akinleye, Toluwanimi E.; Olisaedu, Fredrick E.; Attah, Alfred F.

doi:10.1016/j.phyplu.2021.100019

Cited by 4 publications

(1 citation statement)

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“…The procedure previously reported [16] was followed for the experiment. Nauplii were harvested after hatching by pipette dropping.…”

Section: Lethality Test For Brine Shrimp (Artemia Salina (Bsla)mentioning

confidence: 99%

Peptide-rich extracts from leaves of <i>Newbouldia laevis</i> (P. Beauv.) Seem. ex. Bureau (Bignoniaceae) with antimicrobial and brine shrimp lethality activities

Nkumah,

Kehinde,

Oluremi

et al. 2024

J. Pharm Bio.

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Global concerns over antibiotic-resistant microbial pathogens have spurred a quest for innovative, stable, and target-specific molecules like bioactive peptides. This study evaluates the antimicrobial properties and toxicity of peptide-enriched extracts from Newbouldia laevis leaves. Aqueous extraction, thin layer chromatography (TLC), and Phenomenex's Stratum Giant Tube C18-E were used to obtain partly purified fraction (PPF) and crude peptide extracts (CPE). Antimicrobial inhibitory capacity was determined using p-INT dye, with gentamicin and fluconazole as standards. Cytotoxicity was assessed with Artemia salina nauplii. Microsoft Excel and GraphPad Prism 5 was used in analysing the data. Peptides were detected in CPE and PPF via TLC and modified G-250. PPF demonstrated more efficacy (IC50: 5.89μg/mL to 33.94μg/mL) against bacteria and fungi, with low toxicity (LC50: 5964.87μg/mL for PPFand 1094.20μg/mL for CPE) in the Brine shrimp lethality assay. The study presents the discovery of bioactive peptides from the leaves of Newbouldia laevis. Consequently, bioactive peptides extracted from this plant hold potential as foundational compounds for the development of novel broad-spectrum antibiotics aimed at combating microbial infections.

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“…The procedure previously reported [16] was followed for the experiment. Nauplii were harvested after hatching by pipette dropping.…”

Section: Lethality Test For Brine Shrimp (Artemia Salina (Bsla)mentioning

confidence: 99%