Evaluation of Mycobacterium tuberculosis derived cell-free DNA using pleural fluid and paired plasma samples for the diagnosis of pleural tuberculosis

Sharma, Prafull; Gupta, Rakesh Kumar; Anthwal, Divya; Dass, Manisha; Yadav, Rakesh; Behera, Ashish; Sethi, Sunil; Singhal, Ritu; Dhooria, Sahajal; Aggarwal, Ashutosh N.; Haldar, Sagarika

doi:10.1016/j.tube.2023.102369

Cited by 4 publications

(1 citation statement)

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“…These contemporary sequencing techniques can directly confirm the presence of TB bacilli in the pleural space by identifying their specific gene fragments. Again, the lack of a gold standard means that the performances of these technologies are lower if a composite endpoint, instead of positive culture results, was taken as the final verdict [ 56 , 57 , 58 ▪▪ , 59 , 60 ]. There is thus no consensus on how these techniques are best applied clinically.…”

Section: Diagnostic Investigation To Confirm Tuberculous Pleuritismentioning

confidence: 99%

Tuberculous pleuritis: clinical presentations and diagnostic challenges

Chan,

Lee

2024

Current Opinion in Pulmonary Medicine

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Purpose of review Tuberculous pleuritis (TBP) is one of the most common types of extrapulmonary tuberculosis. We highlight the latest epidemiology of TBP, the heterogeneity of its presentation and the performance of different diagnostic strategies. Recent findings There are differential trends in the incidences of TBP worldwide. Its incidence increased in China but decreased in the United States in the past decade. The presentation of TBP is heterogeneous regarding clinical symptoms, radiological findings and pleural fluid analysis results. Conventional microbiological tests have low sensitivities to diagnose TBP. Recent research focused on various diagnostic tools with better yield. The sensitivity of nucleic acid amplification tests (NAAT) in pleural fluid, including the latest generation of PCR and sequencing-based techniques for detecting tuberculosis, remains suboptimal. Various pleural fluid biomarkers have been explored, but there is a lack of consensus on their clinical utility and cutoff levels. Summary The heterogeneity of clinical presentation poses obstacles to diagnosing TBP. Further development of diagnostic tools, including more robust NAAT and biomarkers with additional validation, is needed before incorporation into routine clinical practice.

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