Evaluation Of Myelotoxicity In Cotton Rats (<i>Sigmodon hispidus</i>) Exposed To Environmental Contaminants. I. In Vitro Bone-Marrow Progenitor Culture

Kim, Soochong; Stair, E L; Lochmiller, Robert L.; Lish, James W.; Qualls, Charles W.

doi:10.1080/009841001455508

Cited by 19 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, some short-term culture assays have been described using rat bone marrow to identify stem cells and committed progenitor cells (Khaldoyanidi et al 1997; Lucas et al 1999). However, these assays have tended to be performed using feeder cells under agar as a source of hemopoietic cytokines and growth factors (Khaldoyanidi et al 1997); alternatively, spleen-or lung-conditioned media in semisolid methylcellulose have been used (Kim et al 2001; Kimura et al 1986).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Near-optimal Conditions for the In Vitro Culture of Hemopoietic Progenitor Cells in Bone Marrow from the Rat

et al. 2009

View full text Add to dashboard Cite

In vitro techniques for the culture of hemopoietic stem cells and committed hemopoietic progenitor cells in rat bone marrow have not been adequately described in the literature. In the present investigations, and using commercially available hemopoietic cytokines and growth factors, the conditions required to perform long-term bone marrow culture (LTBMC) using rat femoral bone marrow were studied, in conjunction with the colony-forming unit cell assay (CFU-C), to quantify the number of progenitor cells. CFU-C production by LTBMCs, set up using Iscove's modified Dulbecco's medium supplemented with fetal calf serum and horse serum, ceased after week 2 of culture. However, the duration of CFU-C production was significantly increased by supplementing LTBMCs with the cytokine recombinant mouse stem cell factor or recombinant rat stem cell factor.

show abstract

Section: Introductionmentioning

confidence: 99%

“…However, these assays have tended to be performed using feeder cells under agar as a source of hemopoietic cytokines and growth factors (Khaldoyanidi et al 1997); alternatively, spleenor lung-conditioned media in semisolid methylcellulose have been used (Kim et al 2001;Kimura et al 1986). …”

Section: Introductionmentioning

confidence: 99%

Near-optimal Conditions for the In Vitro Culture of Hemopoietic Progenitor Cells in Bone Marrow from the Rat

et al. 2009

View full text Add to dashboard Cite

show abstract

Effects of tert–butyl acetate on maternal toxicity and embryo‐fetal development in Sprague‐Dawley rats

Yang¹,

Ahn²,

Lee³

et al. 2007

Birth Defects Research Pt B

View full text Add to dashboard Cite

This study investigated the potential adverse effects of tert-butyl acetate (TBAc) on maternal toxicity and embryo-fetal development after maternal exposure of pregnant rats from gestational days 6 through 19. TBAc was administered to pregnant rats by gavage at 0, 400, 800, and 1,600 mg/kg/day. All dams were subjected to a Caesarean section on day 20 of gestation, and their fetuses were examined for any morphological abnormalities. At 1,600 mg/kg, maternal toxicity manifested as increases in the incidence of clinical signs and death, lower body weight gain and food intake, increases in the weights of adrenal glands and liver, and a decrease in thymus weight. Developmental toxicity included a decrease in fetal weight, an increase in the incidence of skeletal variation, and a delay in fetal ossification. At 800 mg/kg, only a minimal developmental toxicity, including an increase in the incidence of skeletal variation and a delay in fetal ossification, were observed. In contrast, no adverse maternal or developmental effects were observed at 400 mg/kg. These results show that a 14-day repeated oral dose of TBAc is embryotoxic at a maternally toxic dose (i.e., 1,600 mg/kg/day) and is minimally embryotoxic at a nonmaternally toxic dose (i.e., 800 mg/kg/day) in rats. However, no evidence for the teratogenicity of TBAc was noted in rats. It is concluded that the developmental findings observed in the present study are secondary effects to maternal toxicity. Under these experimental conditions, the no-observed-adverse-effect level of TBAc is considered to be 800 mg/kg/day for dams and 400 mg/kg/day for embryo-fetal development.

show abstract

Evaluation of embryo‐fetal development in rats housed in concrete or hwangto cages during pregnancy

Lee¹,

Ahn²,

Yang³

et al. 2008

Birth Defects Research Pt B

View full text Add to dashboard Cite

BACKGROUND:Recently, it was reported that the 4-week exposure of rats to a concrete building environment under cool temperatures (11.7-12.11C) had adverse effects on the general health parameters. This study examined the potential effects of concrete and hwangto building environments on pregnant dams and embryo-fetal development in SpragueDawley rats. METHODS: Groups of 10 mated females were exposed to polycarbonate (control), concrete, or hwangto cages from gestational days (GD) 0 to 20 under cool temperatures (11.9-12.51C). All the females underwent a caesarean section on GD 20, and their fetuses were examined for any morphological abnormalities. RESULTS: The temperatures in the cages were similar in all groups but the relative humidity in the concrete and hwangto groups were higher than in the control group. The concentration of volatile organic compounds in the hwangto group during the study period was lower than in the control group. In the concrete group, maternal effects manifested as an increase in the incidence of clinical signs, a lower body weight, and a decrease in the thymus and ovary weights. Developmental effects included increased post-implantation loss and decreased litter size. In contrast, in the hwangto group, there were no exposurerelated adverse effects on the maternal and developmental parameters. CONCLUSIONS: Overall, the exposure of pregnant rats to a concrete building environment under cool temperatures has adverse effects on the clinical signs, body weight, organ weight, and embryo-fetal development. On the other hand, exposure to a hwangto building environment does not have any adverse effects on pregnant dams or on embryo-fetal development in rats. Birth Defects Res (Part B) 83: 32-39, 2008.

show abstract

Evaluation Of Myelotoxicity In Cotton Rats (Sigmodon hispidus) Exposed To Environmental Contaminants. I. In Vitro Bone-Marrow Progenitor Culture

Cited by 19 publications

References 26 publications

Near-optimal Conditions for the In Vitro Culture of Hemopoietic Progenitor Cells in Bone Marrow from the Rat

Near-optimal Conditions for the In Vitro Culture of Hemopoietic Progenitor Cells in Bone Marrow from the Rat

Effects of tert–butyl acetate on maternal toxicity and embryo‐fetal development in Sprague‐Dawley rats

Evaluation of embryo‐fetal development in rats housed in concrete or hwangto cages during pregnancy

Contact Info

Product

Resources

About