Evaluation of N2‐fixation measured by the15N‐dilution and N‐difference methods in Nicaraguan and EcuadorianPhaseolus vulgarisL. plants inoculated withRhizobium leguminosarumbiovar

Valverde, Gustavo Ramírez; Otabbong, Erasmus

doi:10.1080/09064719709362443

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Crop varieties and rhizobial strain specificities are both significant factors that affect BNF. Strain×cultivar interaction effects on BNF have been reported in lentil (Abi-Ghanem et al 2011), soybean (Israel 1981), common bean (Valverde and Otabbong 1997) and peanut (Wynne et al 1980), but little research has been conducted on pea-related rhizobia-plant interactions (Abi-Ghanem et al 2011). Evaluation of strain×cultivar interaction on current pea varieties would be beneficial for identifying both superior strains and pea breeding lines with genetic superiority in BNF.…”

Section: Introductionmentioning

confidence: 99%

Symbiosis of selected Rhizobium leguminosarum bv. viciae strains with diverse pea genotypes: effects on biological nitrogen fixation

et al. 2017

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Biological nitrogen fixation (BNF) can be improved by optimizing the interaction between the rhizobial inoculant and pea (Pisum sativum L.), leading to increased productivity and reduced nitrogen (N) fertilizer use. Eight Rhizobium leguminosarum bv. viciae strains were used to inoculate the super-nodulating pea mutant Rondo-nod3 (fix+), the hyper-nodulating pea mutant Frisson P88 Sym29, CDC Meadow commercial control, and the non-nodulating mutant Frisson P56 (nod-) to evaluate BNF in a greenhouse assay. Significant differences in strain × cultivar interactions were detected for shoot and root dry masses, which ranged from 1.8 to 4.7 g and from 0.27 to 0.73 g per plant, respectively; for nodule number on lateral roots, which ranged from 25 to 430 per plant; for amount of fixed N, which ranged from 15 to 67 mg and from 4 to 15 mg per plant for shoot and root tissues, respectively; and for percentage of N derived from atmosphere (%Ndfa), which ranged from 37% to 61% and from 35% to 65% for shoot and root tissue, respectively. Strain × cultivar interactions in this study could contribute to identification of superior strains and pea breeding lines with genetic superiority in BNF. Nodule production in pea plants was not necessarily correlated with the amount of fixed N, suggesting nodule activity is more important to BNF than is nodule number.

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Section: Introductionmentioning

confidence: 99%

Symbiosis of selected Rhizobium leguminosarum bv. viciae strains with diverse pea genotypes: effects on biological nitrogen fixation

et al. 2017

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“…Strain x variety interaction is as important as that of strain or crop variety alone (Abi-Ghanem et al, 2011 ). Selection of best microbial strains and plant variety/cultivar is important because a strong effect of cultivar x microbe has been reported on BNF in soybean (Israel, 1981 ; Israel et al, 1986 ), beans (Valverde and Otabbong, 1997 ), peanuts (Wynne et al, 1980 ), lentils and peas (Hafeez et al, 2000 ; Abi-Ghanem et al, 2011 ). Comparison of N 2 fixation among various rhizobial strains demonstrated 42% variability among strains while 81% variability among different lentil cultivars (Hafeez et al, 2000 ).…”

Section: Introductionmentioning

confidence: 99%

Differential response of kabuli and desi chickpea genotypes toward inoculation with PGPR in different soils

et al. 2015

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Pakistan is among top three chickpea producing countries but the crop is usually grown on marginal lands without irrigation and fertilizer application which significantly hampers its yield. Soil fertility and inoculation with beneficial rhizobacteria play a key role in nodulation and yield of legumes. Four kabuli and six desi chickpea genotypes were, therefore, evaluated for inoculation response with IAA-producing Ochrobactrum ciceri Ca-34T and nitrogen fixing Mesorhizobium ciceri TAL-1148 in single and co-inoculation in two soils. The soil type 1 was previously unplanted marginal soil having low organic matter, P and N contents compared to soil type 2 which was a fertile routinely legume-cultivated soil. The effect of soil fertility status was pronounced and fertile soil on average, produced 31% more nodules, 62% more biomass and 111% grain yield than marginal soil. Inoculation either with O. ciceri alone or its co-inoculation with M. ciceri produced on average higher nodules (42%), biomass (31%), grains yield (64%) and harvest index (72%) in both chickpea genotypes over non-inoculated controls in both soils. Soil 1 showed maximum relative effectiveness of Ca-34T inoculation for kabuli genotypes while soil 2 showed for desi genotypes except B8/02. Desi genotype B8/02 in soil type 1 and Pb-2008 in soil type 2 showed significant yield increase as compared to respective un-inoculated controls. Across bacterial inoculation treatments, grain yield was positively correlated to growth and yield contributing parameters (r = 0.294* to 0.838*** for desi and r = 0.388* to 0.857** for kabuli). PCA and CAT-PCA analyses clearly showed a site-specific response of genotype x bacterial inoculation. Furthermore, the inoculated bacterial strains were able to persist in the rhizosphere showing colonization on root and within nodules. Present study shows that plant growth promoting rhizobacteria (PGPR) inoculation should be integrated with national chickpea breading program in Pakistan especially for marginal soils. Furthermore, the study shows the potential of phytohormone producing strain Ca-34T as promising candidate for development of biofertilizer alongwith nodulating strains to get sustainable yield of kabuli and desi chickpea with minimum inputs at marginal land.

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“…Studies of common bean rhizobial diversity in other Mesoamerican countries are scarce. Nicaraguan common bean cultivars were reported to improve their productivity when inoculated with a commercial strain, highlighting the potential of these cultivars for N fixation efficiency [77], and the need for screening native rhizobial strains. In Guatemala, the R. leguminosarum strain CIAT632 was isolated from active nodules of wild and cultivated Mesoamerican common bean cultivars [78], although its predominance in common bean nodules in native Guatemalan soils has not yet been tested.…”

Section: Exploring the Natural Diversity Of Rhizobia Nodulating Commomentioning

confidence: 99%

Natural rhizobial diversity helps to reveal genes and QTLs associated with biological nitrogen fixation in common bean

2017

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Common bean is one of the most important crops for human feed, and the most important legume for direct consumption by millions of people, especially in developing countries. It is a promiscuous host legume in terms of nodulation, able to associate with a broad and diverse range of rhizobia, although the competitiveness for nodulation and the nitrogen fixation capacity of most of these strains is generally low. As a result, common bean is very inefficient for symbiotic nitrogen fixation, and nitrogen has to be supplied with chemical fertilizers. In the last years, symbiotic nitrogen fixation has received increasing attention as a sustainable alternative to nitrogen fertilizers, and also as a more economic and available one in poor countries. Therefore, optimization of nitrogen fixation of bean-rhizobia symbioses and selection of efficient rhizobial strains should be a priority, which begins with the study of the natural diversity of the symbioses and the rhizobial populations associated. Natural rhizobia biodiversity that nodulates common bean may be a source of adaptive alleles acting through phenotypic plasticity. Crosses between accessions differing for nitrogen fixation may combine alleles that never meet in nature. Another way to discover adaptive genes is to use association genetics to identify loci that common bean plants use for enhanced biological nitrogen fixation and, in consequence, for marker assisted selection for genetic improvement of symbiotic nitrogen fixation. In this review, rhizobial biodiversity resources will be discussed, together with what is known about the loci that underlie such genetic variation, and the potential candidate genes that may influence the symbiosis' fitness benefits, thus achieving an optimal nitrogen fixation capacity in order to help reduce reliance on nitrogen fertilizers in common bean.

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Evaluation of N₂‐fixation measured by the¹⁵N‐dilution and N‐difference methods in Nicaraguan and EcuadorianPhaseolus vulgarisL. plants inoculated withRhizobium leguminosarumbiovar

Cited by 4 publications

References 15 publications

Symbiosis of selected Rhizobium leguminosarum bv. viciae strains with diverse pea genotypes: effects on biological nitrogen fixation

Symbiosis of selected Rhizobium leguminosarum bv. viciae strains with diverse pea genotypes: effects on biological nitrogen fixation

Differential response of kabuli and desi chickpea genotypes toward inoculation with PGPR in different soils

Natural rhizobial diversity helps to reveal genes and QTLs associated with biological nitrogen fixation in common bean

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