Evaluation of native and exotic Brazilian plants for anticancer activity

Júnior, Helvécio M. dos Santos; Oliveira, Denilson F.; Carvalho, Daniel Diego Costa; Pinto, Joyce Mendes Andrade; Campos, Viviane Aparecida Costa; Mourão, Ana Raquel Braga; Pessoa, Cláudia; Moraes, Manoel Odoríco de; Costa-Lotufo, Letı́cia V.

doi:10.1007/s11418-010-0390-0

Cited by 44 publications

(25 citation statements)

References 42 publications

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“…According to dos Santos et al [35], natural extracts are considered promising sources of antitumoral compounds when they exhibit IC 50 values lower than 30 µg/mL. That was the case of the J. acutus extract, with an IC 50 value of 6.2 µg/mL in HepG2 cells, significantly lower than that obtained in the S17 cell line (IC 50 , 34.4 µg/mL, p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

Maritime Halophyte Species from Southern Portugal as Sources of Bioactive Molecules

et al. 2014

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Extracts of five halophytes from southern Portugal (Arthrocnemum macrostachyum, Mesembryanthemum edule, Juncus acutus, Plantago coronopus and Halimione portulacoides), were studied for antioxidant, anti-inflammatory and in vitro antitumor properties. The most active extracts towards the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were the methanol extracts of M. edule (IC50 = 0.1 mg/mL) and J. acutus (IC50 = 0.4 mg/mL), and the ether extracts of J. acutus (IC50 = 0.2 mg/mL) and A. macrostachyum (IC50 = 0.3 mg/mL). The highest radical scavenging activity (RSA) against the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical was obtained in the ether extract of J. acutus (IC50 = 0.4 mg/mL) and H. portulacoides (IC50 = 0.9 mg/mL). The maximum total phenolic content (TPC) was found in the methanol extract of M. edule (147 mg gallic acid equivalents (GAE)/g) and in the ether extract of J. acutus (94 mg GAE/g). Significant decreases in nitric oxide (NO) production were observed after incubation of macrophages with lipopolysaccharide (LPS) and the chloroform extract of H. portulacoides (IC50 = 109 µg/mL) and the hexane extract of P. coronopus (IC50 = 98.0 µg/mL). High in vitro cytotoxic activity and selectivity was obtained with the ether extract of J. acutus. Juncunol was identified as the active compound and for the first time was shown to display selective in vitro cytotoxicity towards various human cancer cells.

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Section: Resultsmentioning

confidence: 99%

Maritime Halophyte Species from Southern Portugal as Sources of Bioactive Molecules

et al. 2014

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“…Studies on the medicinal plants in northern Brazil have been well documented in the medical literature, especially as regards Copaiba oil-resin (Tappin et al , 2004; Comelli Júnior et al , 2010), and its manifold therapeutic properties – antiinflammatory, antitumoral, antimelanoma, antiulcerogenic, antilipoperoxidation and antioxidant (Ohsaki et al , 1994; Paiva et al , 2002; Gomes et al , 2007; Silva et al , 2009; dos Santos et al , 2010). Furthermore, new lines of research have been developed, with the aim of analyzing the chemical components involved.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity assessment of Copaiba oil and its fractions in Swiss mice

Almeida¹,

Darin²,

Hernandes³

et al. 2012

Genet. Mol. Biol.

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Copaiba oil-resin, extracted from the trunk of Copaifera, and traditionally used in folk medicine in the treatment of various disorders, has been shown to be an effective antiinflamatory, antitumor, antitetanus, antiseptic and anti-blenorrhagea agent. As, there are few studies evaluating its genotoxicity, this aspect of the commercial oil-resin, and its volatile and resinous fractions, were evaluated in mice by comet assay and micronucleus (MN) test. A single dose of oil resin, volatile or resin fractions (500; 1,000 or 2,000 mg/kg b.w.) was administered by gavage. The chemical compositions of Copaiba oil resin and its fractions was analyzed by gas chromatography. According to comet assaying, treatment with either one did not increase DNA damage, and as to MN testing, there was no alteration in the incidence of micronucleated polychromatic erythrocytes. Chromatographic analysis of the oil-resin itself revealed sesquiterpenes, diterpenic carboxylic acid methyl esters and high levels of β-caryophyllene. Thus, it can be assumed that the oil resin and volatile and resinous fractions from the commercial product are not genotoxic or mutagenic.

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“…MTT assay is a well-characterized colorimetric assay based on the enzymatic reduction of the tetrazolium salt MTT in living, metabolically active cells, but not in dead cells. It has been largely used to determine the cytostatic/cytotoxic potential of medicinal agents in screening programs (Santos-Júnior et al, 2010). The cytotoxicity of the extracts was initially tested against tumour cell lines using the thiazolyl-blue test (MTT) assay at a concentration of 50 μg/mL ( Table 2).…”

Section: Resultsmentioning

confidence: 99%

Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)

et al. 2017

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This study was carried out to assess the antibacterial, cytotoxic and antioxidant activities of extracts of Morus nigra L. HPLC was used to determine the fingerprint chromatogram of the crude ethanolic extract (Mn-EtOH). The antibacterial effect was assessed through the method of microdilution. The cytotoxicity was tested against human tumour cell lines using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The total phenolic and flavonoid contents were also assessed through the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated by using 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging and β-carotene-linoleic acid bleaching methods. The presence of phenolic compounds in Mn-EtOH was confirmed using HPLC. The extracts showed activity against most microorganisms tested. The extracts did not show any expressive antiproliferative effect in the assessment of cytotoxicity. The most significant total phenolic content was 153.00 ± 11.34 mg of gallic acid equivalent/g to the ethyl acetate extract (AcOEt). The total flavonoid content was 292.50 ± 70.34 mg of catechin equivalent/g to the AcOEt extract, which presented the best antioxidant activity (IC 50 50.40 ± 1.16 μg/mL) for DPPH scavenging. We can conclude that this species shows strong antibacterial and antioxidant activities, as well as weak cytotoxic effects.Keywords: antibacterial, antioxidant, cytotoxic, Moraceae, Morus nigra. Avaliação das atividades antibacteriana, citotóxica e antioxidante de Morus nigra L. (Moraceae) ResumoEste estudo foi realizado para avaliar as atividades antibacteriana, citotóxica e antioxidante de extratos de Morus nigra L. HPLC foi utilizado para determinar o perfil de compostos fenólicos do extrato etanólico bruto (Mn-EtOH). O efeito antibacteriano foi avaliado através do método de microdiluição. A citotoxicidade foi testada contra linhagens celulares de tumores humanos utilizando o ensaio do brometo de 3-(4,5-dimetil-2-tiazolil)-2,5-difenil-2H-tetrazólio (MTT). O conteúdo total de compostos fenólicos e flavonoides também foi avaliado por meio dos métodos de Folin-Ciocalteu e cloreto de alumínio, respectivamente. A atividade antioxidante dos extratos foi avaliada por meio do sequestro do radical livre 2,2-difenil-1-picrilhidrazil (DPPH) e co-oxidação do sistema β-caroteno-ácido linoleico. A presença de compostos fenólicos em Mn-EtOH foi confirmada utilizando HPLC. Os extratos mostraram atividade contra a maioria dos microrganismos testados. Os extratos não mostraram qualquer efeito antiproliferativo expressivo na avaliação da citotoxicidade. O conteúdo fenólico total mais significativo foi de 153,00 ± 11,34 mg de equivalente de ácido gálico/g para o extrato acetato de etila (AcOEt). O conteúdo de flavonoides totais foi de 292,50 ± 70,34 mg de equivalente de catequina/g para o extrato AcOEt, que apresentou a melhor atividade antioxidante (IC 50 50,40 ± 1,16 mg/mL) para o sequestro do DPPH. Podemos concluir que esta espécie apres...

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Evaluation of native and exotic Brazilian plants for anticancer activity

Cited by 44 publications

References 42 publications

Maritime Halophyte Species from Southern Portugal as Sources of Bioactive Molecules

Maritime Halophyte Species from Southern Portugal as Sources of Bioactive Molecules

Genotoxicity assessment of Copaiba oil and its fractions in Swiss mice

Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae)

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