Evaluation of nested PCR method for diagnosis of meningitis due to Neisseria meningitidis and Haemophilus influenzae

Bonab, Zahra Hojjati; Farajnia, Safar; Ghotaslou, Reza; Nikkhah, Elhameh

doi:10.3906/biy-1111-27

Cited by 2 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, these samples were also found positive by Real-Time PCR. A similar finding was reported by Bonab ZH et al [17], who had shown culture positivity of 2.5%. The culture positivity rate in CSF decreases by 60-70% in cases of CABM when collected from the patients who have already been taken antibiotics.…”

Section: Discussionsupporting

confidence: 90%

Efficiency of Real-Time PCR in the Diagnosis of Community-Acquired Bacterial Meningitis in Children

Raza

Das

Chaudhry

et al. 2022

J Microbil Infect Dis

View full text Add to dashboard Cite

Objectives: Community-acquired bacterial meningitis (CABM) is a life-threatening condition and remains a public health concern despite various efforts to prevent it. This study aimed to detect the bacteria causing CABM in children by Real-Time PCR. Methods: In total, 178 Cerebrospinal fluid (CSF) samples from suspected meningitis cases were collected and subjected to cell count, biochemical, microbiological, and molecular analysis. Bacteria grown on blood and chocolate agar were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). DNA from CSF was extracted and used to detect bacteria by Real-Time PCR using TaqMan Probe. Results: Fifty (28.09%) patients were diagnosed with confirmed meningitis. Of them, 46 (25.84%) were Real-Time PCR, and four (2.25%) were culture and Real-Time PCR positive. Out of 50 bacteria detected, S. pneumoniae (n=35, 19.7%) was the leading causative bacteria and was followed by H. influenzae (seven, 3.93%), E. coli (five, 2.80%), S. agalactiae (two, 1.12%), and N. meningitidis (one, 0.56%). Most of the S. pneumoniae (18 isolates, 51.4%) were isolated from 3-24 months of children, and in neonates, E. coli was the predominant bacteria. When CSF culture was the gold standard for diagnosis, the sensitivity and specificity of Real-Time PCR for S. pneumoniae were 100% (95%CI: 15.81-100%) and 81.25% (95%CI: 74.69-86.73%), respectively. Conclusion: Streptococcus pneumoniae remains the leading organism of CABM in children despite vaccination and advancement in diagnosis. Real-time PCR has emerged as a vibrant diagnostic molecular appliance. Hence, Regular surveillance is crucial to curb the burdens and trends of CABM in children.

show abstract

Section: Discussionsupporting

confidence: 90%

Efficiency of Real-Time PCR in the Diagnosis of Community-Acquired Bacterial Meningitis in Children

Raza

Das

Chaudhry

et al. 2022

J Microbil Infect Dis

View full text Add to dashboard Cite

show abstract

Impact of Molecular Method for the Diagnosis of Acute Bacterial Meningitis in a Tertiary Health Care Centre in North India

Raza

Das

Goyal

et al. 2020

IJMMR

View full text Add to dashboard Cite

Background. Acute bacterial meningitis is one of the significant infectious diseases that add an immense burden to the health system. Proper management of meningitis is an invincible need to overcome the severe consequences. Objectives. The aim of the study is detection of the etiological agents of acute bacterial meningitis by PCR. Methods. Total 267 CSF samples collected from suspected bacterial meningitis cases were processed for the detection of S. pneumoniae, H. influenzae, N. meningitidis, E. coli and Group B streptococci by conventional and molecular diagnosis method. CSF was inoculated on Blood, chocolate and MacConkey agar plates and incubated at 37 °C for 24-48 hrs. Bacteria grown were identified by Matrix assisted laser desorption/ionization time of flight (MALDI-TOF). Multiplex PCR of the enlisted bacteria was performed using DNA extracted from CSF by DNA extraction kit (Qiagen, USA). Results. 5 (1.87%) out of the total 267 CSF samples were culture positive (3 S. pneumoniae and 2 E. coli) and 28 (10.49%) had detectable DNA by conventional PCR. Out of these 28 samples, 20 (71.43%) were S. pneumoniae and 8 (28.57%) were E. coli. 15 (53.57%) out of total S. pneumoniae were present in children below 5 years of age. Similarly, E. coli was predominant in neonates. Sensitivity and specificity of the PCR was 100% and 95-98% respectively. Conclusions. Streptococcus pneumoniae is the commonest cause of community acquired bacterial meningitis in children below five years of age. Hence, for the promising outcome, PCR should be implemented for the diagnosis of acute bacterial meningitis.

show abstract

Evaluation of nested PCR method for diagnosis of meningitis due to Neisseria meningitidis and Haemophilus influenzae

Cited by 2 publications

References 12 publications

Efficiency of Real-Time PCR in the Diagnosis of Community-Acquired Bacterial Meningitis in Children

Efficiency of Real-Time PCR in the Diagnosis of Community-Acquired Bacterial Meningitis in Children

Impact of Molecular Method for the Diagnosis of Acute Bacterial Meningitis in a Tertiary Health Care Centre in North India

Contact Info

Product

Resources

About