Evaluation of Neuroprotective Effects of Quercetin against Aflatoxin B1-Intoxicated Mice

Gugliandolo, Enrico; Peritore, Alessio Filippo; D’Amico, Ramona; Licata, Patrizia; Crupi, Rosalia

doi:10.3390/ani10050898

Cited by 52 publications

(46 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As previously reported, MDA levels were determined to detect lipid peroxidation four days after administration of DNBS in colon tissue [ 22 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

PEA/Polydatin: Anti-Inflammatory and Antioxidant Approach to Counteract DNBS-Induced Colitis

et al. 2021

Self Cite

View full text Add to dashboard Cite

Palmitoylethanolamide (PEA) has well-known anti-inflammatory effects. However, PEA does not possess an antioxidant ability. A comicronized formulation of ultramicronized PEA (um-PEA) and polydatin (Pol) PEA/Pol, a biological precursor of resveratrol with antioxidant activity, could have protective effects on oxidative stress produced by inflammatory processes. We evaluated the effects of a comicronized PEA/Pol 10 mg/kg (9 mg of um-PEA+1 mg of polydatin) in a model of Dinitrobenzene sulfonic acid (DNBS)-induced colitis. Ulcerative colitis was induced in mice by intrarectally injection of DNBS (4 mg in 100 µL of 50% ethanol per mouse). Macroscopic and histologic colon alterations and marked clinical signs were observed four days after DNBS and elevated cytokine production. The myeloperoxidase (MPO) activity assessed for neutrophil infiltration was associated with ICAM-1 and P-selectin adhesion controls in colons. Oxidative stress was detected with increased poly ADP-ribose polymerase (PARP) and nitrotyrosine positive staining and malondialdehyde (MDA) levels in inflamed colons. Macroscopic and histologic alterations minimized by oral PEA/Pol, as well as neutrophil infiltration, inflammatory cytokine release, MDA, nitrotyrosine, PARP and ICAM-1, and P-selectin expressions. The mechanism of action of PEA/Pol could be related to the sirtuin 1/nuclear factor erythroid 2-related factor 2 (SIRT-1/Nrf2) pathway and nuclear factor (NF)-κB. PEA/Pol administration inhibited NF-κB and increased SIRT-1/Nrf2 expressions. Our results show that PEA/Pol is capable of decreasing inflammatory bowel disease (IBD) DNBS-induced in mice.

show abstract

“…As previously reported, MDA levels were determined to detect lipid peroxidation four days after administration of DNBS in colon tissue [ 22 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

PEA/Polydatin: Anti-Inflammatory and Antioxidant Approach to Counteract DNBS-Induced Colitis

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, being nonspecific, they also bind vitamins and trace elements, generating deficiencies [45][46][47]. Adding some plant-derived antioxidants in feed could be a better solution [48] to diminish the deleterious effects of mycotoxins on animal health. P450 cytochromes enzymes, mainly present in liver, intestinal tract, and kidney, play an important role in phase I biotransformation of xenobiotics, especially those belonging to the families 1 and 3 [49].…”

Section: Introductionmentioning

confidence: 99%

“…However, being nonspecific, they also bind vitamins and trace elements, generating deficiencies [ 45 , 46 , 47 ]. Adding some plant-derived antioxidants in feed could be a better solution [ 48 ] to diminish the deleterious effects of mycotoxins on animal health.…”

Section: Introductionmentioning

confidence: 99%

The Effectiveness of Dietary Byproduct Antioxidants on Induced CYP Genes Expression and Histological Alteration in Piglets Liver and Kidney Fed with Aflatoxin B1 and Ochratoxin A

Popescu

Bulgaru

Untea

et al. 2021

Toxins

View full text Add to dashboard Cite

The purpose of this study was to investigate the potential of a byproduct mixture derived from grapeseed and sea buckthorn oil industry to mitigate the harmful damage produced by ochratoxin A and aflatoxin B1 at hepatic and renal level in piglets after weaning. Forty cross-bred TOPIGS-40 hybrid piglets after weaning were assigned to three experimental groups (E1, E2, E3) and one control group (C), and fed with experimental diets for 30 days. The basal diet was served as a control and contained normal compound feed for starter piglets without mycotoxins. The experimental groups were fed as follows: E1—basal diet plus a mixture (1:1) of two byproducts (grapeseed and sea buckthorn meal); E2—the basal diet experimentally contaminated with mycotoxins (479 ppb OTA and 62ppb AFB1); and E3—basal diet containing 5% of the mixture (1:1) of grapeseed and sea buckthorn meal and contaminated with the mix of OTA and AFB1. After 4 weeks, the animals were slaughtered, and tissue samples were taken from liver and kidney in order to perform gene expression and histological analysis. The gene expression analysis showed that when weaned piglets were fed with contaminated diet, the expression of most analyzed genes was downregulated. Among the CYP450 family, CYP1A2 was the gene with the highest downregulation. According to these results, in liver, we found that mycotoxins induced histomorphological alterations in liver and kidney and had an effect on the expression level of CYP1A2, CYP2A19, CYP2E1, and CYP3A29, but we did not detect important changes in the expression level of CY4A24, MRP2 and GSTA1 genes.

show abstract

“…Quercetin has many biological functions including anti-oxidant, antiinflammatory, and immune-regulatory activities (17)(18)(19). Quercetin supplementation had been shown to attenuate D-Galactose-induced renal damage in rats and aflatoxin B1-induced neurotoxicity in mice through the inhibition of oxidative stress (20,21). A recent study also showed that quercetin supplementation could inhibit lipopolysaccharide (LPS)-induced inflammation in a proximal tubular cell line human kidney 2 (HK-2) cells which derived from normal human kidney (22).…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms Underlying Protective Role of Quercetin on Copper Sulfate-Induced Nephrotoxicity in Mice

et al. 2021

View full text Add to dashboard Cite

Copper overload is an established cause of nephrotoxicity, but the precise molecular mechanism remains unknown. Our study aimed to investigate the molecular mechanism of copper sulfate (CuSO4)-induced nephrotoxicity and the protective effect of the natural compound quercetin using a mouse model. Mice were orally administered CuSO4 only (200 mg/kg per day), or co-administered CuSO4 (200 mg/kg per day) plus quercetin (25, 50, or 100 mg/kg per day), or quercetin only (100 mg/kg per day), or vehicle for 28 days. The blood and kidneys were collected for the examination of serum biomarkers, oxidative stress biomarkers, changes in histopathology and gene and protein expression. Our results show that quercetin supplementation attenuates CuSO4-induced renal dysfunction and tubular necrosis in a dose-dependent manner. Quercetin supplementation at 50 and 100 mg/kg significantly attenuated CuSO4-induced oxidative damage. Quercetin supplementation also inhibited the activities of caspases-9 and−3, and the expression of p53 and Bax mRNAs. Furthermore, quercetin supplementation markedly activated the expression of Nrf2 and HO-1 mRNAs, but inhibited the expression of NF-κB, IL-1β, IL-6, and TNF-α mRNAs. In conclusion, our results revealed that quercetin supplementation could inhibit CuSO4-induced nephrotoxicity in mice via the inhibition of mitochondrial apoptotic and NF-κB pathways and the activation of Nrf2/HO-1 pathway. Our study highlights quercetin as a potential candidate in treating copper overload-induced nephrotoxicity.

show abstract

Evaluation of Neuroprotective Effects of Quercetin against Aflatoxin B1-Intoxicated Mice

Cited by 52 publications

References 64 publications

PEA/Polydatin: Anti-Inflammatory and Antioxidant Approach to Counteract DNBS-Induced Colitis

PEA/Polydatin: Anti-Inflammatory and Antioxidant Approach to Counteract DNBS-Induced Colitis

The Effectiveness of Dietary Byproduct Antioxidants on Induced CYP Genes Expression and Histological Alteration in Piglets Liver and Kidney Fed with Aflatoxin B1 and Ochratoxin A

Molecular Mechanisms Underlying Protective Role of Quercetin on Copper Sulfate-Induced Nephrotoxicity in Mice

Contact Info

Product

Resources

About