Evaluation of Neurotoxicity Potential in Rats: The Functional Observational Battery

Boucard, Aurélie; Bétat, Anne-Marie; Forster, Roy; Simonnard, A.; Froget, Guillaume

doi:10.1002/0471141755.ph1012s51

Cited by 7 publications

(6 citation statements)

References 8 publications

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“…Morris water maze test was performed to evaluate the changes in learning and memory function. FOB is a behavioral screening procedure commonly used in safety pharmacology and toxicology studies to assess the potentially adverse effects of test agents on the central nervous system ( Boucard et al ., 2010 ). In this study, histopathological evaluation was performed to confirm the possibility of neurotoxicity of the tested substances by hematoxylin and eosin staining method from collected brain samples.…”

Section: Discussionmentioning

confidence: 99%

Neurotoxicity of Synthetic Cannabinoids JWH-081 and JWH-210

Jin

Seong

Song

et al. 2015

Biomolecules & Therapeutics

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Synthetic cannabinoids JWH-018 and JWH-250 in ‘herbal incense’ also called ‘spice’ were first introduced in many countries. Numerous synthetic cannabinoids with similar chemical structures emerged simultaneously and suddenly. Currently there are not sufficient data on their adverse effects including neurotoxicity. There are only anecdotal reports that suggest their toxicity. In the present study, we evaluated the neurotoxicity of two synthetic cannabinoids (JWH-081 and JWH-210) through observation of various behavioral changes and analysis of histopathological changes using experimental mice with various doses (0.1, 1, 5 mg/kg). In functional observation battery (FOB) test, animals treated with 5 mg/kg of JWH-081 or JWH-210 showed traction and tremor. Their locomotor activities and rotarod retention time were significantly (p<0.05) decreased. However, no significant change was observed in learning or memory function. In histopathological analysis, neural cells of the animals treated with the high dose (5 mg/kg) of JWH-081 or JWH-210 showed distorted nuclei and nucleus membranes in the core shell of nucleus accumbens, suggesting neurotoxicity. Our results suggest that JWH-081 and JWH-210 may be neurotoxic substances through changing neuronal cell damages, especially in the core shell part of nucleus accumbens. To confirm our findings, further studies are needed in the future.

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Section: Discussionmentioning

confidence: 99%

Neurotoxicity of Synthetic Cannabinoids JWH-081 and JWH-210

Jin

Seong

Song

et al. 2015

Biomolecules & Therapeutics

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“…It includes more than 30 independent observation parameters, which are grouped into domains. The domains are neurological, autonomic, and behavioral; each one with its measurement and evaluation parameters ( Boucard et al, 2010 ). This test is commonly used in studies of neuropathic disorders, and it is easily replicable.…”

Section: Methodsmentioning

confidence: 99%

“…The last evaluations were to determine the righting reflex, after holding the animal by the dorsal region and raising it 30 cm above the test platform (0: fall with all four paws, 1: all paws do not touch the ground, 2: fall laterally, 3: fall). Finally, we evaluated forelimb grip strength positioning the anterior limbs in the cage and performing a slight pull of the body (severe loss of strength: 0; moderate loss: 1; normal: 2; excessive resistance: 3) ( Boucard et al, 2010 ).…”

Section: Methodsmentioning

confidence: 99%

Alcoholic neuropathy associated with chronic alcohol intake

Tessitore

Pereira-Rufino

Panfilio

et al. 2022

IBRO Neuroscience Reports

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“…Visual forelimb-placing response and landing foot splay were tested at three time points: 4 weeks of the age (before surgery), 9 weeks of the age (2 weeks after film implantation), and 11 weeks of the age (4 weeks after film implantation) [22, 23, 24, 25, 26, 27, 28]. As visual placing response, with the tail of a rat was held up, the rat was neared to the flat surface of a table from about 15 cm (Fig.…”

Section: Methodsmentioning

confidence: 99%

Vision evaluation by functional observational battery, operant behavior test, and light/dark box test in retinal dystrophic RCS rats versus normal rats

Matsuo

Uchida

Yamashita

et al. 2019

Heliyon

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Background Vision plays a key role in some behavior tests for rats. Okayama University-type retinal prosthesis (OUReP) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to assess vision in retinal dystrophic (RCS) rats, in comparison with normal rats, by selected behavior tests. We also examined whether the tests could detect vision changes in RCS rats with dye-coupled film implantation. Methods Data sets were 5 normal rats, 4 untreated RCS rats, 7 RCS rats with dye-coupled films implanted at the age of 7 weeks after excluding unsuccessful implantation at autopsy. Behavior tests chosen were landing foot splay and visual forelimb-placing response in the menu of functional observational battery, operant-conditioning lever-press response and light/dark box test. Results Normal visual placing response was significantly less frequent in untreated RCS rats at the age of 9 and 11 weeks, compared with normal rats ( P = 0.0027, chi-square test) while normal response was significantly more frequent at the age of 9 weeks in RCS rats with dye-coupled film implantation, compared with untreated RCS rats ( P = 0.0221). In operant-conditioning lever-press test, the correct response rate was significantly lower in untreated RCS rats than in normal rats at the age of 9 weeks ( P < 0.05, Tukey-Kramer test) while the rate was not significantly different between normal rats and RCS rats with dye-coupled film implantation. In light/dark box test, the time to enter dark box was significantly shorter in normal rats, compared with untreated RCS rats or RCS rats with dye-coupled film implantation ( P < 0.05, Tukey-Kramer test). Conclusions Behavior tests of functional observational battery, operant-conditioning lever-press response and light/dark box test discriminated vision between normal rats and RCS rats. The visual placing response and operant-conditioning lever-press test might have sensitivity to detect vision recovery in RCS rats with OUReP implantation.

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Evaluation of Neurotoxicity Potential in Rats: The Functional Observational Battery

Cited by 7 publications

References 8 publications

Neurotoxicity of Synthetic Cannabinoids JWH-081 and JWH-210

Neurotoxicity of Synthetic Cannabinoids JWH-081 and JWH-210

Alcoholic neuropathy associated with chronic alcohol intake

Vision evaluation by functional observational battery, operant behavior test, and light/dark box test in retinal dystrophic RCS rats versus normal rats

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