Evaluation of New Octacalcium Phosphate-Coated Xenograft in Rats Calvarial Defect Model on Bone Regeneration

Abstract

Bone graft material is essential for satisfactory and sufficient bone growth which leads to a successful implant procedure. It is classified into autogenous bone, allobone, xenobone and alloplastic materials. Among them, it has been reported that heterogeneous bone graft material has a porous microstructure that increases blood vessels and bone formation, and shows faster bone formation than other types of bone graft materials. We observed new bone tissue formation and bone remodeling using Ti-oss® (Chiyewon Co., Ltd., Guri, Korea), a heterologous bone graft material. Using a Sprague–Dawley rat calvarial defect model to evaluate the bone healing effect of biomaterials, the efficacy of the newly developed xenograft Ti-oss® and Bio-Oss® (Geistilch Pharma AG, Wolhusen, Switzerland). The experimental animals were sacrificed at 8 and 12 weeks after surgery for each group and the experimental site was extracted. The average new bone area for the Ti-oss® experimental group at 8 weeks was 17.6%. The remaining graft material was 22.7% for the experimental group. The average new bone area for the Ti-oss® group was 24.3% at 12 weeks. The remaining graft material was 22.8% for the experimental group. It can be evaluated that the new bone-forming ability of Ti-oss® with octacalcium phosphate (OCP) has the bone-forming ability corresponding to the conventional products.

Acceleration of bone formation by octacalcium phosphate composite in a rat tibia critical-sized defect

“…In addition, OCP is a precursor of HA [ 16 ]. Previous studies have investigated OCP use in the calvaria, mandible, and frontal bones, which has led to the approval of commercially available products for use in dentistry [ [17] , [18] , [19] , [20] ]. OCP studies in the lower extremities have previously examined tibial bone hole defects and critical-sized defects in a rat model; however, none have investigated alginate or collagen composites [ 10 , [21] , [22] , [23] ].…”

The potential bone regeneration effects of leptin- and osteolectin-coated 3D-printed PCL scaffolds: an in vivo study

“…Short-term studies, typically lasting 4-8 weeks, are often performed to assess the early stages of bone healing and regeneration. Long-term studies, sometimes lasting several weeks or months, are also performed to assess the early and intermediate stages of bone regeneration and complete regeneration of the calvarial defect [54,55,73,74]. Therefore, an 8-week period was chosen to evaluate the initial bone formation and healing stages.…”

“…The skin and periosteum were then lifted to expose them. Next, a drill equipped with a trephine bur (diameter 5.5 mm: non-critical size defect) was used to create two circular calvarial defects, resulting in 30 calvarial defects [54,55]. The PCL, PCL/Leptin and PCL/Osteo scaffolds (n = 10 per group) were then inserted into the calvarial defects (figure 2).…”

Simultaneous Implant and Guided Bone Regeneration Using Bovine-Derived Xenograft and Acellular Dermal Matrix in Aesthetic Zone

“…This method results in a porous structure akin to cancellous bone in humans, offering high osteoconduction due to its gradual and beneficial absorption that defends the space [ 46 ]. The absorption of xenografts, particularly deproteinized bovine bone mineral (DBBM), plays a crucial role in maintaining the volume of space, as excessive absorption may delay the formation of new bone [ 30 ]. To address this concern, researchers have focused on developing materials with high biocompatibility that mirror the remodeling characteristics of human bones.…”

“…Following the attainment of primary stability, decortication was performed around the bone to stimulate additional intraosseous vascularization, thereby promoting the angiogenesis process ( Figure 2 C) [ 29 ]. Afterwards, the bone defect area received a bovine-derived xenograft (Ti-oss, Chiyewon, Republic of Korea) combined with autografts taken during implant oseteotomy and extracted from the buccal bone area using a scraper ( Figure 2 D) [ 20 ] ( Figure 2 D) [ 22 , 30 ]. Subsequently, the ADM was meticulously positioned to secure the bone graft in place, and the matrix was fixated using resorbable sutures (Luxcryl PDO, Lux Sutures, Luxembourg) ( Figure 2 E).…”