Evaluation of Nickel–Zinc Interactions by Means of Bioassays with Amphibian Embryos

Herkovits, Jorge; Pérez-Coll, Cristina S.; Herkovits, F.D.

doi:10.1006/eesa.1999.1857

Cited by 32 publications

(17 citation statements)

References 60 publications

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“…1), which implies that, for certain developmental stages, the exposure to Cu concentrations of about LC10 or LC50 may represent a risk for 50% or 90% of the population, respectively. Similar results were obtained for other chemicals in certain exposure conditions [24,28]. In the case of Cu, these facts are of special concern because of the very small difference from the LC10 to the LC50 or from the LC50 to the LC90 in certain developmental stages.…”

Section: Discussionsupporting

confidence: 83%

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

Aronzon

Sandoval

Herkovits

et al. 2011

Enviro Toxic and Chemistry

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Copper toxicity in different embryonic and larval stages of the common South American toad Rhinella arenarum was evaluated by means of continuous and 24-h pulse treatments in 12 different developmental stages. Lethal concentrations (LC) of 10, 50, and 90% of continuous treatment with Cu from early blastula (S.4), complete operculum (S.25), and hind limb bud (S.28) stages were plotted from 24 to 168 h, resulting from S.4 in a 24-h LC50 of 137 µg Cu(2+) /L and a 168-h LC50 of 19.5 µg Cu(2+) /L. This result was in agreement with pulse treatments that showed a high resistance to Cu at blastula and gastrula stages, whereas the organogenic period, between muscular response (S.18) and open mouth (S.21), was very susceptible to this metal. Continuous treatments from S.25 showed no significant differences along exposure time (168-h LC50 = 51 µg Cu(2+) /L), but in the case of S.28 toxicity increased slightly from a 24-h LC50 of 138.6 µg Cu(2+) /L to a 168-h LC50 of 104 µg Cu(2+) /L, pointing out that, although the larval period was significantly more resistant to Cu, there was also a remarkable stage-dependent susceptibility to this metal. Copper teratogenic potential was approximately two, and main adverse effects were reduced body size, axial flexure, microcephaly, acephaly, mouth malformations, agenesis of or underdeveloped gills, agenesis of or underdeveloped tail, and hydropsy. The results are discussed considering Cu toxicity mechanisms, an evolutionary perspective, and environmental protection.

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Section: Discussionsupporting

confidence: 83%

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

Aronzon

Sandoval

Herkovits

et al. 2011

Enviro Toxic and Chemistry

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“…However, conversely to the rapid effects recorded in the case of other heavy metals, such as Cu and Cd, the lethality for Ni increased sharply between 72 and 96 h [8]. The TOP curves also reveal an asymmetry in the LC10 and LC50 versus LC50 and LC90 that seems to point out that a limited number of individuals have a remarkable resistance to Ni toxicity, a feature reported also for other species [29] Although most toxicological studies evaluating adverse effects on early life stages are conducted by means of continuous treatments [7], evaluation of the stage-dependent susceptibility to noxious agents could provide meaningful information on the remarkable changes during the ontogenic process [17,18] related to the succession of very diverse and complex features of cell differentiation and morphogenetic processes.…”

Section: Discussionmentioning

confidence: 60%

“…Freshwater organisms have a wide range of sensitivity to Ni; for example, the 50% lethal concentrations (LC50s) for acute exposure are 0.05 for some algae and 263 mg Ni 2þ /L for rainbow trout [2]. In the case of amphibians, a study with Xenopus laevis embryos employing FETAX (frog embryo teratogenesis assay-Xenopus), reported a LC50 96 h of 8.9 mgNi 2þ /L [7]; in the South American toad (Rhinella arenarum) tadpole, the acute and short-term chronic toxicity at complete operculum stage resulted in LC50s of 3.62 and 1.79 mg Ni 2þ /L, respectively [8]. Amphibians play a key role in the ecosystem because of the trophic implications of their complex life cycle, turning from aquatic herbivores to terrestrial predators.…”

Section: Introductionmentioning

confidence: 99%

Nickel toxicity in embryos and larvae of the South American toad: Effects on cell differentiation, morphogenesis, and oxygen consumption

Sztrum

D'Eramo

Herkovits

2011

Enviro Toxic and Chemistry

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Nickel, a widely distributed heavy metal in the biosphere, produces systemic, carcinogenic, and teratogenic effects. The objectives of the present study are to report the acute, short-term chronic, and chronic toxicity of Ni in Rhinella arenarum embryos as well as the stage-dependent susceptibility to this heavy metal, including oxygen consumption, teratogenesis, and adverse effects on cell differentiation processes. The stages evaluated were blastula (S.7), gastrula (S.11), tail bud (S.17), fin circulation (S.22), and complete operculum (S.25), in this last case by means of toxicity profile curves. Nickel increases its adverse effects gradually, with a maximum value after 96 h. The 50% lethal concentrations (LC50s) for 96, 168, and 240 h at S.25 were 1.14, 0.60, and 0.48 mg Ni²(+) /L, respectively; S.11 and S.22 were the least and most susceptible to Ni with, LC50s 96 h of 6.12 and 0.19 mg Ni²(+) /L, respectively. A reduction of approximately 25% in oxygen consumption anticipates lethal effects from S.17 onward. The main teratogenic effects were retarded growth and development, extremely severe axis incurvations, persistent yolk plug, asymmetry, microcephaly and mouth and gill agenesia, and limited neuromuscular activity. Ciliated cells were not functional. The possibility of associating the remarkable stage-dependent susceptibility to Ni with environmental changes during the evolutionary process is also considered.

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“…On the other hand, Zn 2þ present in tilapias may act as a protective agent against the toxic effects of heavy metals (Vargas, Paulino, and Nozaki 2009). This protective effect of Zn 2þ against heavy metal toxicity was previously reported (Herkovits, Pe´rez-Coll, and Herkovits 2000;Tapiero and Tew 2003). The high amount of Zn 2þ released from the paint of tank resulted in a high concentration of Zn 2þ in both water and feces.…”

Section: Determination Of Al 3y By Dpasvmentioning

confidence: 91%

Toxic effects of dietary of Al³⁺ ions in tilapias (Oreochromis niloticus) and protective effect of Zn²⁺ ion

Santos

Paulino

Tambourgi

et al. 2010

Toxicological & Environmental Chemistry

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Evaluation of Nickel–Zinc Interactions by Means of Bioassays with Amphibian Embryos

Cited by 32 publications

References 60 publications

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

Nickel toxicity in embryos and larvae of the South American toad: Effects on cell differentiation, morphogenesis, and oxygen consumption

Toxic effects of dietary of Al³⁺ ions in tilapias (Oreochromis niloticus) and protective effect of Zn²⁺ ion

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Evaluation of Nickel–Zinc Interactions by Means of Bioassays with Amphibian Embryos

Cited by 32 publications

References 60 publications

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

Nickel toxicity in embryos and larvae of the South American toad: Effects on cell differentiation, morphogenesis, and oxygen consumption

Toxic effects of dietary of Al3+ ions in tilapias (Oreochromis niloticus) and protective effect of Zn2+ ion

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Toxic effects of dietary of Al³⁺ ions in tilapias (Oreochromis niloticus) and protective effect of Zn²⁺ ion