Evaluation of Nigella Sativa Seeds on Broiler Chicks Hematological, Blood Biochemical Parameters and Antioxidant Enzymes

Abstract

The present study targets to look deep at the influence of Nigella sativa seeds (NSS) supplementation on hematological, blood biochemical measurements, liver and kidney functions and antioxidant enzymes of Arbor Acres broiler chicks. A total of 140 unsexed one day old Arbor Acres broiler chicks were randomly divided into four treatment groups during the period from 1-35 days of age. The chicks were randomly assigned in a straight run experimental design among four treatments, each replicated 7 times with 5 unsexed chicks per replicate. The 1 st group was fed a commercial basal diet without any supplementation (control), the 2 nd , 3 rd and 4 th groups were fed control diet supplemented with NSS at 0.5, 1.0 and 1.5% levels. Chicks fed basal diet and basal diet without supplementation or with 1.5% NSS had significantly higher RBCs than the 0.5 and 1% NSS supplemented groups. Chicks fed the NSS at 0.5% showed a higher plasma albumin level than the control group, 1 and 1.5 NSS groups. Feeding diet supplemented with 1% NSS had significantly (P≤0.05) achieved the lowest triglyceride and cholesterol compared with chicks fed diet that contained 0.5 and 1.5% NS seeds groups. Significantly (P≤0.05) higher high-density lipoprotein (HDL) was recorded in chick fed diet that contained 1.5% NSS compared with the control and the other treatments. Chicks fed diet that contained 1% NSS had significantly (P≤0.05) achieved a lower plasma aspartate amino transferase (AST). All of supplementation with different NSS levels had a significantly (P≤0.05) higher plasma creatinine and lower plasma urea to creatinine ratio than the control group. Chicks fed diet contained 0.5% NSS had significantly (P≤0.05) achieved a higher plasma superoxide dismutase and glutathione peroxidase activity compared with the control, 1 and 1.5% NSS supplemented groups. Chicks fed the diet containing 1% NSS showed higher plasma glutathione level than the control group, 0.5 and 1.5% NSS groups. Feeding diet with supplemented 0.5 and 1.5% NSS were significantly (P≤0.05) increased plasma glutathione reductase and catalase compared with the control and 1% NSS groups. Conclusion: These findings indicated that NSS had improved lipid profile, liver function and antioxidant profile of broiler chicks. Thus, 1% NSS is considered safe due to having no acute toxic side effects as reported through the experimental period.

Interactive effects of dietary protein and nano-chitosan on growth performance, immune response, and histological aspects of lymphoid organs in broiler chickens

“…Our results agree with those of Abou El-Maaty et al ( 2021 ), who reported significant increases in serum concentrations of the antioxidant enzymes (CAT and SOD) and FSH and T3 hormones in broiler chickens fed diets containing 7.5% RSM compared with their control birds. Consistent with the present results, Hassan ( 2021 ) found that feeding diets containing Nigella sativa seeds to broiler chicks caused a significant increase in blood plasma activity of catalase and glutathione reductase compared with the control group. It is well-known that thyroid hormones are vital regulators of metabolic processes and that T3 is the active form of these hormones.…”

The effects of black cumin (Nigella sativa l..) seed on carcass characteristics, kidney oxidant antioxidant levels and ileum histomorphology in Japanese quails

“…These results are consistent with the result of the present study that supplementation of 0.5%, 1%, and 2% NS to the diet did not affect slaughter, hot carcass, wing, liver and heart weights, compared to the control group.In some studieswith broilers; Amadand Mohammed(2013) found that the addition of 1.5%, 2.5%, and 3.5% NS did not affect slaughter weight, carcass yield, and edible visceral rates; Kumar et al (2018) stated that slaughter weight, carcass yield, breast, thigh, wing, neck, liver, and abdominal fat weight, and total edible visceral weight increased or tended to increase with the addition of 0.5%, 1% and 2% black cumin Although kidney MDA values showed a tendency to decrease in the NS groups, this decrease was not significant compared to the control group. Similar to this study, Hassan (2021) reported that 0.5%, 1%, and 1.5% black cumin supplementation did not affect the plasmaMDA level in broiler chickens. Likewise, Aydoganet al (2020) reported that 0.5% NS supplementation did not affect serum total antioxidant and oxidant levels.…”

“…In studies similar to this study, it has been reported that thymoquinone, the main active ingredient of black cumin, 6000 F. KAZAK, T. CIMRIN, S. ALASAHAN, M.A. KISACAM, T. KUTLU increases CAT and GPx activities (El-Hack et al, 2018;Harzallah et al, 2012;Hassan, 2021;Sogut et al, 2008). It has also been reported to have lipid peroxidation inhibitory effects and antioxidant activity (Harzallah et al, 2012).…”

“…This study shows that black cumin can be effective for detoxification of hydrogen peroxide, which occurs with the increase in renal CAT activities, and the contribution of black seed to nutritional performance. Hassan (2021) stated that there is an improvement in the health status of broiler chickens due to the fact that black cumin increases antioxidant enzymes. The present study suggests that renal GPx activity increases with black cumin supplementation and that GPx activity is used to maintain the oxidant-antioxidant balance.…”

“…In the literature, there are mostly studies examining the effects of using NS in quail feeds on the performance, food digestibility and economic efficiency of quails (Karadagoglu et al, 2019;Tufan et al, 2015). In addition, in previous studies, serum oxidant and antioxidant levels of NS in broiler feeds were examined (Aydogan et al, 2020;Hassan, 2021). However, in the literature reviews, the number of studies related to the effect of the usage of NS in quail rations on carcass properties, kidney oxidant-antioxidant parameters and ileum histomorphology in quails was extremely insufficient.…”