Evaluation of Nitric Oxide Scavenging and Nitric Oxide Synthases Expression of Resveratrol and Polydatin

Abstract

Cellular damage induced by free-radicals like reactive oxygen species(ROS) has been implicated in several diseases. The ROS radicals like alkoxy radical(RO-) and peroxy radical (ROO-) are similar to those that are physiologically active and thus might initiate a cascade of intracellular toxic events leading to DNA damage and cell death. Hence naturally anti-oxidant play a vital role in combating these conditions. Resveratrol (RES) and polydatin (PD) are two kinds of natural phytoalexin with excellent antimicrobial and anti-inflammatory activity. In this study aim to evaluation the antiinflammatory ability of RES and PD on LPS-stimulate inflammatory cell model by Nitric oxide(NO) colorimetric method and NO synthases expression with qRT-PCR method, to provide a basis for the potential application and development in the future. The results showed that RES is significantly stronger than PD in NO synthases expression (P < 0.05). At the same time, the comparison of NO production inhibition rate between RES and PD are not significant (P > 0.05). RES and PD show their respective anti-inflammatory activity advantages under different indicators, which means we should choose RES or PD according to the different uses.