Evaluation of Non-polar Composition in Plumbago Zeylanica Leaves by Gas Chromatography and Mass Spectrometry

Abstract

Background:Plumbago zeylanica plant belongs to Plumbaginaceae. The plant is reported for many pharmacological activities. Aim: The objective of the study was to identify fatty acids and non-polar chemical compounds in Plumbago zeylanica leaves. Materials and methods: Petroleum ether extract was prepared using soxhlet apparatus. Saponifiable and unsaponifiable matter was separated with saponification process. To identify fatty acids in saponifiable matter further esterification was performed. Gas chromatography and mass spectrometry analysis was performed of both saponifiable and unsaponifiable fractions. All the fatty acid methyl esters and non-polar chemical compounds were identified using NIST library data. Results: A total of 14 compounds were identified with comparison of NIST data. From that, 8 fatty acid methyl esters and 6 non-polar chemical compounds were identified. Here we have analyzed fatty acids and non-polar chemical compounds by the same GC-MS method. Conclusions: The present analysis showed that Plumbago Zeylanica leaves contain 8 fatty acids and 6 non-polar chemical compounds. Principal determination of the research was development of efficient method to identify non-polar compound from plant by single injection using chromatographic technique.

Identification of Chemical Compounds in Plumbago zeylanica Linn Leaves from Indonesia

“…[13][14][15] A molecular docking study reported that -sitosterol has activity against Protein Kinase C. 16 This conclusion is not coincidental with the results of Dhalani et al (2020), who identified -sitosterol, Taraxasterol, and Lanosterol as nonpolar molecules in P. zeylanica L. leaves, but not -sitosterol. 17 This study also found Lupeol in the chloroform extract of PZCJ as shown in Table 2. It is known as a multi-target agent, similar to Plumbagin, and has biological activities as anticancer and anti-inflammatory.…”

Establishment of quality parameters of Quisqualis indica leaves through sophisticated analytical techniques

“…Agilent 5977B MS occupied to 7820A GC was used to analyze. HP-5 capillary column (30 m × 0.32 mm; 0.25 µm film thickness) was used for GC-MS studies [12][13][14][15][16]. NIST library of the GC-MS was used to identify the detected compounds.…”