Evaluation of normal ocular bacterial flora with two different culture media

Moeller, Cecília Tobias de Aguiar; Branco, Bruno Castelo; Yu, Maria Cecília Zorat; Farah, Michel Eid; Santos, Manoel Armando Azevedo dos; Höfling-Lima, Ana Luísa

doi:10.1016/s0008-4182(05)80004-3

Cited by 37 publications

(9 citation statements)

References 11 publications

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“…35 Topical or systemic antibiotics can also decrease the percentage of positive cultures. 36 Judging from above, we found it difficult to make good prediction on MK based on periodic conjunctival swab cultures. In addition, conjunctival swab culture may not be as sensitive as corneal scraping cultures.…”

Section: Discussionmentioning

confidence: 86%

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Microbial Keratitis in Patients With Stevens–Johnson Syndrome and Toxic Epidermal Necrolysis: Experience From a Tertiary Centre in Taiwan

Tsai

Adiyabazar

Hsiao

et al. 2022

Cornea

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Section: Discussionmentioning

confidence: 86%

“…In addition, conjunctival swab culture may not be as sensitive as corneal scraping cultures. [36][37][38] Therefore, culture reports from corneal scrapings remain the irreplaceable guidance for treatment until other new diagnostic methods are introduced in the future.…”

Section: Discussionmentioning

confidence: 99%

Microbial Keratitis in Patients With Stevens–Johnson Syndrome and Toxic Epidermal Necrolysis: Experience From a Tertiary Centre in Taiwan

Tsai

Adiyabazar

Hsiao

et al. 2022

Cornea

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“…Several studies have evaluated the ocular surface microbiome and/or microbiota by using traditional microbiology methods [ 33 , 34 ], but the current knowledge is still growing thanks to the complex analyses performed by genomic technology [ 19 , 35 ]. The metagenomic analysis permits the characterization of the entire microbial community present in a sample as well as the definition of the richness and evenness of this community, although the method does not discriminate between viable and non-viable microbes.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Healthy Eye Microbiota Niche in a Multicenter Study

Borroni

Paytuví-Gallart

Sanseverino

et al. 2022

IJMS

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Purpose: This study aims to explore and characterize healthy eye microbiota. Methods: Healthy subjects older than 18 years were selected for this descriptive cross-sectional study. Samples were collected with an eSwab with 1 mL of Liquid Amies Medium (Copan Brescia, Italy). Following DNA extraction, libraries preparation, and amplification, PCR products were purified and end-repaired for barcode ligation. Libraries were pooled to a final concentration of 26 pM. Template preparation was performed with Ion Chef according to Ion 510, Ion 520, and Ion 530 Kit-Chef protocol. Sequencing of the amplicon libraries was carried out on a 520 or 530 chip using the Ion Torrent S5 system (Thermo Fisher; Waltham, MA, USA). Raw reads were analyzed with GAIA (v 2.02). Results: Healthy eye microbiota is a low-diversity microbiome. The vast majority of the 137 analyzed samples were highly enriched with Staphylococcus, whereas only in a few of them, other genera such as Bacillus, Pseudomonas, and Corynebacterium predominate. We found an average of 88 genera with an average Shannon index of 0.65. Conclusion: We identified nine different ECSTs. A better understanding of healthy eye microbiota has the potential to improve disease diagnosis and personalized regimens to promote health.

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“…Conjunctival culture positivity ranges between 30.0-93.0% (4) , (10). Staphylococcus epidermidis is known to be the most densely colonized bacterium in the conjunctival ora (11).…”

Section: Discussionmentioning

confidence: 99%

An Investigation Into the Conjunctival and Nasal Flora of Patients Receiving Cyclosporine Eye Drop Treatment for Dry Eye

Elibol

habip

Elbay

et al. 2022

Preprint

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OBJECTIVEThe aim of this study is to analyze the long-term use of cyclosporine drops of anti-inflammatory effect on conjunctival and nasal flora, which are commonly used in dry eye treatment, , to examine antibiotic resistance and to suggest some precautions.METHODSThe 38 people using cyclosporine drops were classified as Group 1, and the 34 people using preservative-free artificial tear preparations were classified as Group 2. Swabs were taken from the conjunctiva and nasal cavities of the volunteers participating in the study and cultured. Groups 1 and 2 were compared in terms of growing microorganisms and antibiotic susceptibility.RESULTSThere was no statistically significant difference between Groups 1 and 2 in terms of bacteria growing in the eye and nasal cavities. When it comes to the antibiotic susceptibility statistics for S. epidermidis, low sensitivity to penicillin was observed in particular in both groups, but there was no significant difference between the two groups (p=0.036)CONCLUSIONThe use of cyclosporine or preservative-free artificial tears did not make any difference to the flora or to the antibiotic susceptibility of the flora. It is thought that the use of cyclosporine drops does not predispose to ocular infection.

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Evaluation of normal ocular bacterial flora with two different culture media

Cited by 37 publications

References 11 publications

Microbial Keratitis in Patients With Stevens–Johnson Syndrome and Toxic Epidermal Necrolysis: Experience From a Tertiary Centre in Taiwan

Microbial Keratitis in Patients With Stevens–Johnson Syndrome and Toxic Epidermal Necrolysis: Experience From a Tertiary Centre in Taiwan

Exploring the Healthy Eye Microbiota Niche in a Multicenter Study

An Investigation Into the Conjunctival and Nasal Flora of Patients Receiving Cyclosporine Eye Drop Treatment for Dry Eye

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