Evaluation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb.

Mendes, Amanda Ferreira da Silva; Cidade, Luciana C.; Oliveira, Maria Luiza Peixoto de; Otoni, Wagner Campos; Soares-Filho, Walter dos Santos; Costa, Márcio Gilberto Cardoso

doi:10.1007/s11240-009-9518-x

Cited by 18 publications

(12 citation statements)

References 14 publications

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“…1). Da Silva Mendes et al [13] observed that meropenem at 6.25 mg L -1 was insufficient to completely inhibit the growth of strains EHA 101 and EHA 105 16 h after bacterial inoculation, and suggested the use of a higher concentration (12.5 mg L -1 ) for the control of β-lactamase-producing strains. Considering the results from the presented study, it is important to be aware of the observation that the low concentrations of meropenem could inhibit bacterial growth for a limited period of time only, as can be observed in the case of cefotaxime or timentin, at a concentration of 100 mg L -1 .…”

Section: Resultsmentioning

confidence: 99%

“…This group of antibiotics act by interfering with penicillin-binding proteins and by inhibiting the biosynthesis of the peptidoglycan network of the cell wall that provokes cell wall lysis and consequently bacterial death [12]. However, it has been shown that the application of β-lactam antibiotics frequently affects plant growth and development, leading to either the inhibition [13,14] or the promotion [15][16][17][18] of regeneration ability. They may differently affect callus induction [14], shoot formation [19], somatic embryogenesis [20] or rooting [13].…”

Section: Introductionmentioning

confidence: 99%

“…However, it has been shown that the application of β-lactam antibiotics frequently affects plant growth and development, leading to either the inhibition [13,14] or the promotion [15][16][17][18] of regeneration ability. They may differently affect callus induction [14], shoot formation [19], somatic embryogenesis [20] or rooting [13]. It is known that plant sensitivity to antibiotics is species-, genotype-or even tissue-specific [21].…”

Section: Introductionmentioning

confidence: 99%

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The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious

Stanišić

Ninković

Savić

et al. 2018

ARCH BIOL SCI BELGRA

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Since the genetic transformation of the apple is strongly genotype-dependent and generally inefficient, the evaluation of factors affecting shoot regeneration are crucial for the establishment of a successful transformation process. In this report, we evaluated the effects of the β-lactam antibiotics meropenem and timentin on in vitro regeneration via de novo shoot organogenesis from leaf explants of apple cv. Golden Delicious, as well as on the growth of the Agrobacterium tumefaciens strain EHA 105, and compared them with the commonly used β-lactam cefotaxime. Also, we report for the first time the effect of hygromycin B as a selective agent in the domesticated apple, as regards shoot regeneration and shoot multiplication efficiency. We observed that cefotaxime and timentin at concentrations higher than 100 mg L -1 were sufficient to prevent Agrobacterium growth during a two-week period, while meropenem exhibited an inhibitory effect on bacterial growth at all tested concentrations (25-150 mg L -1 ). Cefotaxime at a concentration of 300 mg L -1 increased the number of regenerated shoots per explant (9.39) in comparison with the control (7.67). In contrast to cefotaxime, meropenem and timentin caused a decrease in shoot regeneration efficiency, but larger and more developed shoots were obtained on meropenem (25-125 mg L -1 ) after the same period of cultivation. Hygromycin B at a concentration of 5 mg L -1 or higher completely inhibited shoot regeneration and induced explant tissue necrosis. Therefore, the selection procedure with a final concentration of 4 mg L -1 throughout organogenesis and 10 mg L -1 for further shoot growth and multiplication is recommended for an efficient transformation process in apple cv. Golden Delicious. Abbreviations: BAP -N 6 -benzilaminopurin; BM − basal medium; IBA -indole-3-butyric acid; MIC -minimal inhibitory concentration; MS -Murashige and Skoog mineral solution; NAA -α-naphthaleneacetic acid; HPThygromycin B phosphotransferase; SFC -the index of shoot-forming capacity; SMM -shoot multiplication medium; SRM -shoot regeneration medium; TDZ -thidiazuron How to cite this article: Stanišić M, Ninković S, Savić J, Ćosić T, Mitić N. The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious. Arch Biol Sci. 2018;70(1):179-90.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious

Stanišić

Ninković

Savić

et al. 2018

ARCH BIOL SCI BELGRA

View full text Add to dashboard Cite

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“…Beta-lactam antibiotics, such as cefotaxime, are the most commonly used antibiotics in plant transformation protocols, since they have a broad spectrum of activity against bacteria and a low toxicity to eukaryotes (Yu et al, 2001). Although inhibitory effect on shoot regeneration have already been previously reported Mendes et al, 2009), stimulatory effects have also been reported for some species such as Zea mays (Danilova and Dolgikh, 2004) and Citrus (Oliveira et al, 2010). Stimulatory effect of antibiotics on plant morphogenesis may be explained by their chemical structure, which may mimic plant growth regulators (Nakano and Mii, 1993), or their degradation by-products, which may generate metabolites with plant growth regulator activity (Mathias and Mukasa, 1987).…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium-mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene

Franco¹,

Gomes²,

Filho³

et al. 2016

Afr. J. Biotechnol.

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Jatropha curcas L. oil has been shown to be suitable for the production of biodiesel. However, this species has not been domesticated yet. Genetic breeding through conventional methods is time consuming and costly, hence, genetic transformation could contribute positively to the improvement of interesting traits. Although in vitro regeneration and stable genetic transformation has been pursued for several years, variation in transformation efficiency remains strongly genotype-dependent and indicates that protocols optimization is still needed. Thus, this study was carried out to introduce a chitinase gene from the Trichoderma viride fungus into the genome of a J. curcas superior genotype by inoculating leaf explants with Agrobacterium tumefaciens EHA 105 strain in axenic conditions. Some key parameters such as pre-culture period and antibiotic doses were optimized with 500 mg.L -1 cefotaxime and 100 mg.L -1 kanamycin concentrations being suitable for A. tumefaciens inhibition and explant selection, respectively. The best transformation efficiency (50%) was obtained when leaf explants were incubated on a culture medium promoting shoot regeneration at 15 days before the induction of the transformation process. Plants where chitinase gene amplicons could be detected were assessed for transgene copy number and expression levels by quantitative real-time polymerase chain reaction (PCR). One, two and three copies of the introduced gene were confirmed in nine transgenic lines with two of them that were assessed for gene expression and showed quantitative variation for this variable. These results bring valuable information for further gene insertions in breeding programs of J. curcas for fungal disease resistance.

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“…Unfortunately, noticeable inhibitory effects of these antibiotics, mainly carbenicillin and cefotaxime, on plant cell growth, organogenesis and embryogenesis have been well documented (Nauerby et al 1997;Ogawa and Mii 2005;Wiebke et al 2006;Mendes et al 2009). Recently, many blactams have been developed to enhance and expand antibacterial activity (Demain and Elander 1999).…”

Section: Effects Of B-lactam Antibiotics On Secondary Sementioning

confidence: 99%

Studies on genetic transformation of Theobroma cacao L.: evaluation of different polyamines and antibiotics on somatic embryogenesis and the efficiency of uidA gene transfer by Agrobacterium tumefaciens

Silva

Cidade

Alvim

et al. 2009

Plant Cell Tiss Organ Cult

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In order to develop a more efficient genetic transformation system for cacao somatic embryos, the effects of polyamines and b-lactam antibiotics on somatic embryogenesis, hygromycin as selective agent, and different factors affecting uidA gene transfer have been evaluated. The polyamines putrescine, spermidine, and spermine significantly improved secondary somatic embryogenesis in cacao. Spermine at 1,000 lM provided the best responses, increasing 6.79 the percentage of embryogenic callus and 2.59 the average number of embryos per embryogenic callus. The b-lactam antibiotics timentin and meropenem, used for Agrobacterium tumefaciens counter-selection, had a nondetrimental effect on secondary somatic embryogenesis, depending on their concentration, whereas the commonly used b-lactam cefotaxime inhibited it, irrespective of the tested concentration. Hygromycin showed a strong inhibitory effect on secondary somatic embryogenesis of cacao, impairing completely the embryo production at 20 mg l -1 . Following the criterion of GUS activity, the best conditions for T-DNA transfer into cotyledon explants from primary somatic embryos of cacao were a sonication of the explants for 100 s, a 20-min incubation period in Agrobacterium solution, an Agrobacterium concentration of 1.0 (OD 600 ), and cocultivation of the explants on tobacco feeder layers. These findings will have important implications for studies on functional genomics of cacao.

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Evaluation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb.

Cited by 18 publications

References 14 publications

The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious

The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious

Agrobacterium-mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene

Studies on genetic transformation of Theobroma cacao L.: evaluation of different polyamines and antibiotics on somatic embryogenesis and the efficiency of uidA gene transfer by Agrobacterium tumefaciens

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