Limited feed for pigs encourages using alternative feed such as tamarind seeds using hydroponic fodder technology. The research aimed to examine the nutrient content of tamarind seed hydroponic fodder due to different watering frequencies. The research was conducted in Kolhua Village, Kupang City from April-September 2023. The research used tamarind seeds originating from the Lembata city. The research used a Completely Randomized Design, with 3 watering frequency treatments, namely R0 = 1 watering (07.00 WIT), R1 = 2 times (07.00 and 19.00), R2 = 3 times (07.00, 15.00 and 23.00 Wita) and 8 repetitions. There were 100 seeds per container. Variables include tamarind seed fodder's nutrient content (organic matter, crude protein, crude fiber, crude fat, and ash). Analysis used Analysis of Variance and Duncan's advanced test. The research results showed that watering frequency had a very significant effect (P<0.01) on the nutrient levels of tamarind seed hydroponic fodder except for ash content and the watering frequency that produced the best nutrient levels was twice/day. In conclusion, the germination percentage and nutrient content of tamarind seed hydroponic fodder are influenced by watering frequency twice/day with 30 ml being the best frequency.