Evaluation of Ogataea (Hansenula) polymorpha for Hyaluronic Acid Production

Manfrão-Netto, João Heitor Colombelli; Queiroz, Enzo Bento; Rodrigues, Kelly Assis; Coelho, Cíntia Marques; Paes, Hugo Costa; Rech, E. L.; Parachin, Nádia Skorupa

doi:10.3390/microorganisms9020312

Cited by 6 publications

(4 citation statements)

References 57 publications

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“…Distinctive cells with moderate aggregation were also observed. A similar cellular structure and aggregation were earlier reported for H. polymorpha by Manfrão-Netto et al ( 2021) [42].…”

Section: Sem Imagingsupporting

confidence: 88%

“…The Saccharomyces cerevisiae yeast is conventionally used in the fermentation process due to its high ethanol production, tolerance for a broad pH range, and high ethanol concentration [24,25]. The major challenges in the conventional fermentation process are the rise in temperature (35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45) • C) and ethanol content (above 20%), which inhibit the growth and productivity of yeasts. The metabolic activities of microbes generate heat stress during the fermentation process, which causes defects in and the reduced growth of microbes.…”

Section: Introductionmentioning

confidence: 99%

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Fermentation of Sugar by Thermotolerant Hansenula polymorpha Yeast for Ethanol Production

Karim,

Martínez-Cartas,

Cuevas-Aranda

2024

Fermentation

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Hansenula polymorpha is a non-conventional and thermo-tolerant yeast that is well-known for its use in the industrial production of recombinant proteins. However, research to evaluate this yeast’s potential for the high-temperature fermentation of sugar to produce alcohols for biofuel applications is limited. The present work investigated a wild-type H. polymorpha strain (DSM 70277) for the production of ethanol at a temperature of 40 °C under limited oxygen presence by using a batch fermentation reactor. Fermentation experiments were performed using three types of sugar (glucose, fructose, xylose) as substrates with two initial inoculum concentrations (1.1 g·L−1 and 5.0 g·L−1). The maximum specific growth rates of H. polymorpha yeast were 0.121–0.159 h−1 for fructose, 0.140–0.175 h−1 for glucose, and 0.003–0.009 h−1 for xylose. The biomass volumetric productivity was 0.270–0.473 g·L−1h−1 (fructose), 0.185–0.483 g·L−1h−1 (glucose), and 0.001–0.069 g·L−1h−1 (xylose). The overall yield of ethanol from glucose (0.470 g·g−1) was higher than that from fructose (0.434 g·g−1) and xylose (0.071 g·g−1). The H. polymorpha yeast exhibited different behavior and efficacy regarding the use of glucose, fructose, and xylose as substrates for producing ethanol. The present knowledge could be applied to improve the fermentation process for valorization of waste biomass to produce bioethanol.

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“…Distinctive cells with moderate aggregation were also observed. A similar cellular structure and aggregation were earlier reported for H. polymorpha by Manfrão-Netto et al ( 2021) [42].…”

Section: Sem Imagingsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Fermentation of Sugar by Thermotolerant Hansenula polymorpha Yeast for Ethanol Production

Karim,

Martínez-Cartas,

Cuevas-Aranda

2024

Fermentation

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“…Among HA heterologous producing micro‐organisms, the only eukaryotes that have been exploited so far are yeasts, specifically Komagataella phaffii (1.7 g L −1 and 1.2 MDa), Kluyveromyces lactis (1.89 g L −1 and 2.09 MDa) and Ogataea polymorpha (0.198 g L −1 ) (Gomes et al, 2019; Jeong et al, 2014; Manfrão‐Netto et al, 2021). The absence of other studies aimed at improving HA production in yeasts is probably due to the complexity of HA synthesis in these organisms.…”

Section: Improving Ha Titres In Heterologous Producersmentioning

confidence: 99%

Genetic strategies for improving hyaluronic acid production in recombinant bacterial culture

Manfrão-Netto

Queiroz

Junqueira

et al. 2021

J of Applied Microbiology

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Hyaluronic acid (HA) is a biopolymer of repeating units of glucuronic acid and Nacetylglucosamine. Its market was valued at USD 8.9 billion in 2019. Traditionally, HA has been obtained from rooster comb-like animal tissues and fermentative cultures of attenuated pathogenic streptococci. Various attempts have been made to engineer a safe micro-organism for HA synthesis; however, the HA titres obtained from these attempts are in general still lower than those achieved by natural, pathogenic producers. In this scenario, ways to increase HA molecule length and titres in already constructed strains are gaining attention in the last years, but no recent publication has reviewed the main genetic strategies applied to improve HA production on heterologous hosts. In light of that, we hereby compile the advances made in the engineering of micro-organisms to improve HA synthesis.

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“…Apart from that, its thermotolerance and strong methanol-inducible promoters also make it a promising host organism for producing platform chemicals derived from C1 molecules [ 14 , 15 ]. There are several recent studies, in which industrially relevant chemicals, such as free fatty acids [ 16 ], hyaluronic acid [ 17 ], and the terpenoid β-elemene [ 18 ] were produced in O. polymorpha .…”

Section: Introductionmentioning

confidence: 99%

Methanol bioconversion into C3, C4, and C5 platform chemicals by the yeast Ogataea polymorpha

Wefelmeier,

Schmitz,

Kösters

et al. 2024

Microb Cell Fact

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Background One carbon (C1) molecules such as methanol have the potential to become sustainable feedstocks for biotechnological processes, as they can be derived from CO2 and green hydrogen, without the need for arable land. Therefore, we investigated the suitability of the methylotrophic yeast Ogataea polymorpha as a potential production organism for platform chemicals derived from methanol. We selected acetone, malate, and isoprene as industrially relevant products to demonstrate the production of compounds with 3, 4, or 5 carbon atoms, respectively. Results We successfully engineered O. polymorpha for the production of all three molecules and demonstrated their production using methanol as carbon source. We showed that the metabolism of O. polymorpha is well suited to produce malate as a product and demonstrated that the introduction of an efficient malate transporter is essential for malate production from methanol. Through optimization of the cultivation conditions in shake flasks, which included pH regulation and constant substrate feeding, we were able to achieve a maximum titer of 13 g/L malate with a production rate of 3.3 g/L/d using methanol as carbon source. We further demonstrated the production of acetone and isoprene as additional heterologous products in O. polymorpha, with maximum titers of 13.6 mg/L and 4.4 mg/L, respectively. Conclusion These findings highlight how O. polymorpha has the potential to be applied as a versatile cell factory and contribute to the limited knowledge on how methylotrophic yeasts can be used for the production of low molecular weight biochemicals from methanol. Thus, this study can serve as a point of reference for future metabolic engineering in O. polymorpha and process optimization efforts to boost the production of platform chemicals from renewable C1 carbon sources.

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Evaluation of Ogataea (Hansenula) polymorpha for Hyaluronic Acid Production

Cited by 6 publications

References 57 publications

Fermentation of Sugar by Thermotolerant Hansenula polymorpha Yeast for Ethanol Production

Fermentation of Sugar by Thermotolerant Hansenula polymorpha Yeast for Ethanol Production

Genetic strategies for improving hyaluronic acid production in recombinant bacterial culture

Methanol bioconversion into C3, C4, and C5 platform chemicals by the yeast Ogataea polymorpha

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