Evaluation of optimal survival of primitive progenitor cells (LTC-IC) from PBPC apheresis products after overnight storage

Abstract

Summary:Optimal overnight (ON) storage of PBPC aphereses is becoming an increasingly important issue and different options for storing PBPC products exist. The survival of primitive progenitor cells is of major interest, as recent data suggest that these progenitors are not only important for long-term engraftment but also contribute significantly to the early phase of hematopoietic engraftment after myeloablative therapy. We therefore investigated the survival of primitive progenitor cells (ie long-term culture initiating cells, LTC-IC) before (ie within 2 h after finishing the apheresis procedure) and after ON storage lasting 16 to 20 h. In addition, we compared the % of recovery of LTC-IC with that of mature progenitors (ie colony-forming cells, CFC) and with the % viability of the mononuclear cells in the apheresis product. Aliquots of PBPC aphereses products were tested in collection bags at room temperature (RT), in EDTA tubes both at RT or 4؇C ؎ the addition of autologous plasma (AP; 2.6-fold the apheresis volume) and ؎ the possibility of gas exchange. Mean viable cell counts did not show strong differences between the different storage conditions and were poor predictors for the survival of CFC and LTC-IC. At RT (collection bags, EDTA tubes ؎ gas exchange) recoveries (% of input) of both, CFC (18%, 18% and 31%) and LTC-IC (10%, 4%, 17%) were low. The addition of AP at RT improved the survival of CFC and LTC-IC to 66% and 38%, respectively. Optimal recoveries for both types of progenitors (CFC: 99%, LTC-IC: 109%) were obtained at 4؇C in the presence of AP. In addition, a good correlation between the survival of CFC and LTC-IC was obtained (r = 0.76) suggesting that the analysis of CFC may also allow some conclusions to be drawn on the survival of LTC-IC. Bone Marrow Transplantation (2000) 25, 197-200.

Validation of short‐term handling and storage conditions for marrow and peripheral blood stem cell products

“…A number of previous studies have indicated that short‐term storage of PBSCs at a refrigerated temperature does not affect TNC and CFU recovery nor trypan blue viability 16‐20 . Many also found refrigerated temperatures to be optimal for recovery of colony‐forming and long‐term culture‐initiating cells during short‐term storage 15,21,22 . Two other studies also suggested maintenance of cell viability after overnight storage of PBSC products at refrigerated temperatures 23,24 .…”

Low concentrations of STI571 in the cerebrospinal fluid: a case report

“…Numbers and genotypes of mature and primitive progenitor cells (i.e. colony forming cells, CFC and long‐term culture‐initiating cells, LTC‐IC) in the BM were analysed as described (Gunsilius et al , 2000; Petzer et al , 2000).…”

Low‐oxygen and high‐carbon‐dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia

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