Evaluation of Oregano (Lippia berlandieri) Essential Oil and Entomopathogenic Fungi forVarroa destructorControl in Colonies of Honey Bee,Apis mellifera

Romo-Chacón, Alejandro; Martínez-Contreras, Liz J.; Molina-Corral, Francisco Javier; Acosta-Muñíz, Carlos H.; Rios‐Velasco, Claudio; León-Door, Adrián Ponce de; Rivera, Raúl

doi:10.3958/059.041.0427

Cited by 18 publications

(11 citation statements)

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“…Essential oils are environmentally friendly and, as their mechanisms of action are involved in different molecular pathways, it is rare for treated parasitic populations to develop resistance mechanisms. In particular, the essential oil tested has provided data in line with those reported in other works previously published for other species of oregano [ 45 , 61 , 62 , 69 ].…”

Section: Discussionsupporting

confidence: 89%

“…The authors applied oregano oil (Sigma ® , Missasauga, ON, Canada) to hives using electric vaporizers (in vivo study), obtaining a reduction in the degree of infestation close to 97.4% [19]. These results are markedly better than those obtained by Romo-Chacón et al (2016) with an average mortality between 57-74% by soaking a cotton towel with a solution containing oregano (Lippia berlandieri) essential oil [61]. A high mortality rate was obtained in tests performed by Gal et al (1992) [62] which, in any case, resulted lower than that obtained by Sabahi's group [35].…”

Section: Discussionmentioning

confidence: 94%

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Green Veterinary Pharmacology for Honey Bee Welfare and Health: Origanum heracleoticum L. (Lamiaceae) Essential Oil for the Control of the Apis mellifera Varroatosis

Castagna

Bava

Piras

et al. 2022

Veterinary Sciences

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Varroatosis, caused by the Varroa destructor mite, is currently the most dangerous parasitic disease threatening the survival of honey bees worldwide. Its adverse effect on the welfare and health of honey bees requires the regular use of specific acaricides. This condition has led to a growing development of resistance phenomena towards the most frequently used drugs. In addition, another important aspect that should not be understated, is the toxicity and persistence of chemicals in the environment. Therefore, the identification of viable and environmentally friendly alternatives is urgently needed. In this scenario, essential oils are promising candidates. The aim of this study was to assess the contact toxicity, the fumigation efficacy and the repellent effect of Origanum heracleoticum L. essential oil (EO) against V. destructor mite. In the contact tests, each experimental replicate consisted of 15 viable adult female mites divided as follows: 5 treated with EO diluted in HPLC grade acetone, 5 treated with acetone alone (as negative control) and 5 treated with Amitraz diluted in acetone (as positive control). The EO was tested at concentrations of 0.125, 0.25, 0.5, 1 and 2 mg/mL. For each experimental replicate, mortality was manually assessed after one hour. The efficacy of EO fumigation was evaluated through prolonged exposure at different time intervals. After each exposure, the 5 mites constituting an experimental replicate were transferred to a Petri dish containing a honey bee larva and mortality was assessed after 48 h. The repellent action was investigated by implementing a directional choice test in a mandatory route. During the repellency tests the behavior of the mite (90 min after its introduction in the mandatory route) was not influenced by the EO. In contact tests, EO showed the best efficacy at 2 and 1 mg/mL concentrations, neutralizing (dead + inactivated) 90.9% and 80% of the mites, respectively. In fumigation tests, the mean mortality rate of V. destructor at maximum exposure time (90 min) was 60% and 84% at 24 and 48 h, respectively. Overall, these results demonstrate a significant efficacy of O. heracleoticum EO against V. destructor, suggesting a possible alternative use in the control of varroatosis in honey bee farms in order to improve Apis mellifera welfare and health and, consequently, the hive productions.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 94%

Green Veterinary Pharmacology for Honey Bee Welfare and Health: Origanum heracleoticum L. (Lamiaceae) Essential Oil for the Control of the Apis mellifera Varroatosis

Castagna

Bava

Piras

et al. 2022

Veterinary Sciences

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“…They also found residues of carvacrol in honey samples from their treated colonies that were below the 1.1 mg/kg taste threshold (Imdorf et al 1999 ). We did not analyze samples of honey and wax for residues of oregano and clove oils from this study, but our group collected samples of honey and wax from a preliminary study of colonies treated with oregano oil and the levels of this compound in the samples were <0.16 mg/kg (Gashout et al unpublished data), which agrees with the study of Romo-Chacón et al ( 2016 ).…”

Section: Discussionsupporting

confidence: 87%

“…In other studies, it was found that the application of a mixture of several oils, including oregano oil impregnated in cardboard strips, provided low mite control in temperate climates (Sammataro et al 1998 ; Skinner et al 2001 ). Finally, Romo-Chacón et al ( 2016 ) applied oregano oil impregnated in cotton towels, finding 57–74% efficacy for varroa mite control. They also found residues of carvacrol in honey samples from their treated colonies that were below the 1.1 mg/kg taste threshold (Imdorf et al 1999 ).…”

Section: Discussionmentioning

confidence: 99%

Continuous release of oregano oil effectively and safely controls Varroa destructor infestations in honey bee colonies in a northern climate

et al. 2017

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The ectoparasitic mite Varroa destructor is responsible for the death of millions of honey bee (Apis mellifera) colonies worldwide. Testing potential miticide compounds with different delivery methods that effectively control V. destructor and have low toxicity for honey bees is crucial to manage this parasite in hives. We determined the varroacide efficacy of three natural compounds delivered to hives with three application methods over a 4-week period. Oxalic acid in a sucrose solution was applied impregnated in cardboard (T1). A mixture of oregano and clove oils in an ethanol-gelatin solution was applied impregnated in absorbent pads (T2). Oregano oil alone was delivered using electric vaporizers (T3) to test the hypothesis that continuous release of miticides increases the varroacidal efficacy of essential oils. The varroa mite control rates for treatments T1–T3 were 76.5 ± 7.11, 57.8 ± 12.79 and 97.4 ± 0.68%, respectively, and there were no differences for bee mortality between control and treatments 1 and 3. Additionally, most mites were killed in the first 2 weeks in T3 colonies compared to the last 2 weeks in colonies of the other treatments. These results demonstrate the importance of continuously releasing natural miticides to achieve safe and high rates of mite control in hives. They also show that oregano oil may be an effective miticide against V. destructor infestations in colonies.

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“…Recientemente, se menciona a compuestos orgánicos del fruto de Azadirachta indica, con efectos de replencia y varroacidas (González- Gómez et al, 2006) y Guazuma ulmifolia, con reportes de efectividad entre 41-69 % (May-Itzá et al, 2019). A la vez, el aceite de Lippia berlandieri (orégano) (Romo-Chacón et al,2016), extractos de Laurus nobilis (laurel) y Alium sativum (ajo) (Reyes-Fuentes et al, 2022), presentan efectividad acaricida entre el 57, 42.46 y 25.67 %, respectivamente, sobre V. destructor.…”

Section: Porcentaje De Efectividadunclassified

EFECTO ACARICIDA DE Pachyrhizus erosus SOBRE Varroa destructor EN COLONIAS DE Apis mellifera

Trujillo¹,

Romero

Rodríguez

2023

Trop Subtrop Agroecosyst

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Background: The main problem of beekeeping activity in Mexico is parasitosis by Varroa destructor, due to its wide distribution and the damage it produces in apiaries, causing mortality in bees and reduction in honey production levels. Therefore, it is essential to develop sanitary control strategies for varroasis, avoiding causing the presence of resistant vectors, harmful damage to bees, beekeepers, consumers or any type of surrounding contamination or in the main products of the hive. Objective: To evaluate the acaricidal activity of Pachyrhizus erosus (jicama) on the mite V. destructor in colonies of Apis mellifera. Methodology: For the obtain of the extract from mature jicama seed, the hot maceration/extraction method (bain-marie) was used, using ethanol 95° G.L (95%) as solvent. A completely random design was developed. randomized with two dilutions of P. erosus (5 and 10%), a negative control (distilled water) and a positive control (Cumavar®), with three repetitions, respectively. The treatments were applied by means of two plastic strips (between frames 6 and 10) impregnated with the corresponding concentrations. Results: The highest count of accumulated mites was presented in the positive control with 386 (128.67±22.74). During the sampling, an initial infestation rate of 4.54±1.64 was determined. On the other hand, the final infestation rate was 2.66±2.69. The effectiveness of the treatments was statistically significant among themselves. The 5 and 10% dilutions of the P. erosus seed extract presented an acaricidal effect with percentages greater than 45% (48.99±15.1 and 59.44±13.57% of effectiveness, respectively). Likewise, the positive control presented the highest percentage, 84.41±14.47. Implications: Trials should be developed with a greater number of experimental units and records with a longer post-application time, with the aim of evaluating their residual and harmful effect that these may have on non-target individuals such as bees. Conclusion: Exploratory data on the acaricidal effect of P. erosus seed on V. destructor and its application as a means of controlling varroa infestation were presented, as a feasible alternative for the development of strategies for the sanitary control of varroa.

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Evaluation of Oregano (Lippia berlandieri) Essential Oil and Entomopathogenic Fungi forVarroa destructorControl in Colonies of Honey Bee,Apis mellifera

Cited by 18 publications

References 24 publications

Green Veterinary Pharmacology for Honey Bee Welfare and Health: Origanum heracleoticum L. (Lamiaceae) Essential Oil for the Control of the Apis mellifera Varroatosis

Green Veterinary Pharmacology for Honey Bee Welfare and Health: Origanum heracleoticum L. (Lamiaceae) Essential Oil for the Control of the Apis mellifera Varroatosis

Continuous release of oregano oil effectively and safely controls Varroa destructor infestations in honey bee colonies in a northern climate

EFECTO ACARICIDA DE Pachyrhizus erosus SOBRE Varroa destructor EN COLONIAS DE Apis mellifera

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