Evaluation of organic-inorganic hybrid nanoflower's enzymatic activity in the presence of different metal ions and organic solvents

Altınkaynak, Cevahir; Gülmez, Canan; Atakisi, Onur; Özdemir, Nalan

doi:10.1016/j.ijbiomac.2020.07.118

Cited by 33 publications

(13 citation statements)

References 40 publications

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“…The relative activity of β‐GNF was lower than the free β‐glucosidase at 30 and 50 °C, but β‐GNF at high temperature (70 °C) maintained its activity more than 63 %, while free enzyme retained ∼47 % of their activities. Similar findings were reported in several nanoflower studies [24–26] . The fact that the β‐GNF exhibits better activity than free enzyme in low acidic and alkaline environment and at high temperature can be considered as an important advantage in industrial processes that require harsh reaction environments.…”

Section: Resultssupporting

confidence: 87%

Glucose Tolerance, Antiprotease Activity and Total Oxidant/Antioxidant Capacity Studies of β‐Glucosidase Hybrid Nanoflower for Industrial Applications

Gülmez¹

2022

Chemistry & Biodiversity

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β‐Glycosidases, which catalyse the hydrolysis of glycoside bonds, have a wide spectrum of industrial applications. However, the reaction product glucose inhibits the activities of many β‐glucosidases. Consequently, the reduced catalytic activities of the enzyme limit the industrial applications of the enzymes. For that reason, the studies dealing with maintaining the activities of the relevant enzymes at high glucose concentrations are a great interest among the researchers. In this context, herein, protein‐inorganic hybrid nanoflowers were synthesized using β‐glucosidase and copper ion by fast sonication method for 10 min. After characterization of synthesized nanoflowers, pH/temperature studies, glucose tolerance, anti‐protease activity, recyclability and total antioxidant and total oxidative capacity levels were estimated. Accordingly, the optimum pHs of free β‐glucosidase and hybrid nanoflower (β‐GNF) were found to be 6 and 5, respectively, and the optimum temperature values for both hybrid nanoflowers and free enzyme were 40 °C. β‐GNF exhibited better activity than free enzyme in low acidic and alkaline environment and at high temperature. The nanoflower retained nearly all (99 %) of its initial activity at all glucose concentrations (0.01, 0.05 and 0.1 mg/mL), especially at pH 5 and 6. Also, β‐GNF maintained more than 90 % of initial activity at 0.01 and 0.05 mg/mL glucose at pH 4 and 7. It also displayed about 96 % high residual activity after proteinase K treatment for 3 h at 37 °C, while that of the free β‐glucosidase was about 87 %. The reusability studies showed that β‐GNF only lost ∼28 % of its initial activities at the end of five cycles. The hybrid nanoflowers at 5 mg/mL concentration exhibited the high total antioxidant capacity. In addition, low total oxidant capacity and oxidative stress index levels were recorded at the same concentration of the hybrid nanoflower. The findings of the present study revealed that β‐GNFs may be evaluated as a candidate for various industrial applications due to its high glucose tolerance, anti‐protease activity, reusability and resistance to low acidic/alkaline environment and high temperature.

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Section: Resultssupporting

confidence: 87%

Glucose Tolerance, Antiprotease Activity and Total Oxidant/Antioxidant Capacity Studies of β‐Glucosidase Hybrid Nanoflower for Industrial Applications

Gülmez¹

2022

Chemistry & Biodiversity

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“…In the pH range of 5.0–8.0 there is a significant difference in the activity of the derivatives, demonstrating that incorporation of metallic particles influences enzyme immobilization. The main advantage of metal ions is to favor the immobilization of the enzyme in a more favorable conformation, regulating the orientation of the active site; and they also play a role in its stability, enabling the increase in enzymatic activity 65,66 . These results showed that functionalization of activated carbon via incorporation of metallic particles was effective.…”

Section: Resultsmentioning

confidence: 88%

“…The main advantage of metal ions is to favor the immobilization of the enzyme in a more favorable conformation, regulating the orientation of the active site; and they also play a role in its stability, enabling the increase in enzymatic activity. 65,66 These results showed that functionalization of activated carbon via incorporation of metallic particles was effective. A pH value of 7.0 for the reaction medium was selected as optimal since it provides maximum activity of the derivatives: 71.9 ± 1.1 U for CCAC-PPL and 79.8 ± 1.7 U for CCACM-PPL.…”

Section: Effect Of Ph On the Immobilization Processmentioning

confidence: 87%

Incorporation of metallic particles in activated carbon used in lipase immobilization for production of isoamyl acetate

Oliveira

Santos

Brito

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND: Biotechnology has revolutionized the use of enzymes for application in industrial processes. The present work evaluated the effect of functionalizing activated carbon with metallic nanoparticles obtained from corn cob residue on lipase immobilization. The aim was to improve the enzymatic activity of the immobilized enzyme and subsequent application of derivatives in the synthesis of isoamyl acetate ester (banana flavor). RESULTS:The activated carbon (CCAC) had a mesoporous structure and specific surface area of 1135 m 2 g −1 , characteristic of supports from precursor material with a high cellulose content, which was reduced in metalized carbon (CCACM) due to the insertion of metallic particles (760 m 2 g −1 ). CCACM showed a slightly higher lipase absorptive capacity than CCAC (280.72 ± 0.27 mg g −1 and 276.46 ± 0.03 mg g −1 , respectively). Furthermore, for derivatives the optimal reaction medium conditions were pH 7.0 and 30 °C, confirming the advantage associated with lipase immobilization, obtaining activity values of 84.0 ± 2.12 U, 72.3 ± 1.7 U and 31.4 ± 1.9 U, for lipase immobilized on CCACM, CCAC and native form, respectively. The derivatives showed potential application in the synthesis of isoamyl acetate ester, with conversions above 90% and stability during five cycles of use. CONCLUSION:The activated carbon metallization process showed an increase in lipase immobilization efficiency and proved to be a promising support, since there was an increase in its enzymatic activity and the derivatives obtained showed potential application in aroma synthesis, reaching conversion above 90%.

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“…[34,35] Evaluating Total Antioxidant and Total Oxidative Capacities of Nanoflowers Previous reports have clearly revealed the potential applications of nanoflowers in electrochemical biosensors, and catalyst. [2,3,23,24,36] Up to our best knowledge and survey, there is very little information about AOC and toxicity of nanoflowers. Herein, total antioxidant and antioxidant capacity levels and oxidative stress indexes of different concentrations of nanoflowers were assayed.…”

Section: Hemolytic Assay Of Nanoflowersmentioning

confidence: 99%

Hemoglobin‐Inorganic Hybrid Nanoflowers with Different Metal Ions as Potential Oxygen Carrying Systems

Gülmez

Altınkaynak

Turk

et al. 2021

Chemistry & Biodiversity

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Protein-inorganic hybrid nanoflowers have tremendous potential in bionanotechnology due to their simple method of preparation, high stability and superior properties. Considering these features, the present study was designed to investigate the artificial blood substitution potentials of hemoglobin-inorganic hybrid nanoflowers. In this context, hemoglobin-inorganic hybrid nanoflowers (Cu-NF, Co-NF and Zn-NF) were synthesized using with different metal ions (copper, cobalt and zinc), then their oxygen carrying capacity, the hemolytic studies, in vitro oxidant/antioxidant capacity levels and oxidative stress index were reported for the first time. The present findings have revealed that Zn-NF had significant oxygen content and artificial oxygen carriers (AOC), as well as a significantly low percent hemolysis rate and a safe standard value. Also, hemolysis rate decreased along with the increases in hemoglobin content coupled with increments in nanoflower concentrations. The percentage hemolysis rate was lower than all nanoflowers at low free hemoglobin concentration, but hemolysis rates also increased with increments in concentration. The results showed that in general, Zn-NF stands out with its high total antioxidant capacity and low total oxidant capacity and oxidative stress index. The obtained results showed that Cu-NF and Co-NF, especially Zn-NF might be considered as a potential superior artificial oxygen carrier. Therefore, this nanoflower system might be act as an efficient material as a blood substitute in the near future.

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Evaluation of organic-inorganic hybrid nanoflower's enzymatic activity in the presence of different metal ions and organic solvents

Cited by 33 publications

References 40 publications

Glucose Tolerance, Antiprotease Activity and Total Oxidant/Antioxidant Capacity Studies of β‐Glucosidase Hybrid Nanoflower for Industrial Applications

Glucose Tolerance, Antiprotease Activity and Total Oxidant/Antioxidant Capacity Studies of β‐Glucosidase Hybrid Nanoflower for Industrial Applications

Incorporation of metallic particles in activated carbon used in lipase immobilization for production of isoamyl acetate

Hemoglobin‐Inorganic Hybrid Nanoflowers with Different Metal Ions as Potential Oxygen Carrying Systems

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