Evaluation of oxidative stress and antioxidant status: Correlation with the severity of sepsis

Abstract

Sepsis is a condition caused by infection followed by unregulated inflammatory response which may lead to the organ dysfunction. During such condition, over-production of oxidants is one of the factors which contribute cellular toxicity and ultimately organ failure and mortality. Antioxidants having free radicals scavenging activity exert protective role in various diseases. This study has been designed to evaluate the levels of oxidative and antioxidative activity in sepsis patients and their correlation with the severity of the sepsis. A total of 100 sepsis patients and 50 healthy controls subjects were enrolled in this study from the period October 2016 to June 2017. The investigation included measurements of oxidative enzyme, myeloperoxidase (MPO), antioxidant enzymes including superoxide dismutase activity (SOD) and catalase activity (CAT) and cytokines (TNF-α, IL-8 and IFN-γ). Furthermore, the level of these activities was correlated with severity of sepsis. Augmented levels of oxidants were found in sepsis as demonstrated by DMPO nitrone adduct formation and plasma MPO level activity (1.37 ± 0.51 in sepsis vs 0.405 ± 0.16 in control subjects). Cytokines were also found to be increased in sepsis patients. However, plasma SOD and CAT activities were significantly attenuated (P < .001) in the sepsis patients compared with controls subjects. Moreover, inverse relation between antioxidant enzymes (SOD and CAT) and organ failure assessment (SOFA), physiological score (APACHE II), organ toxicity specific markers have been observed as demonstrated by Pearson's correlation coefficient. This study suggests that imbalance between oxidant and antioxidant plays key role in the severity of sepsis.

Hydrocortisone, Ascorbic acid and Thiamine (HAT Therapy) for the Treatment of Sepsis. Focus on Ascorbic Acid

“…[20] Similarly, Kumar et al reported augmented levels of oxidants in patients with sepsis as demonstrated by DMPO nitrone adduct formation and plasma myeloperoxidase (MPO) level activity. [21] In this study the SOFA and APACHE II scores correlated linearly with the MPO levels and inversely with the levels of antioxidants. This study supports the concept that the imbalance between oxidant and antioxidants plays key role in the pathophysiology of organ failure in sepsis.…”

Lipidomic profile and candidate biomarkers in septic patients

“…There is an alteration to the pro-oxidant-antioxidant balance. Also, there is an increase in the concentration of inflammatory cytokines (TNF-α and IL-8) and a decrease in the plasma activity of superoxide dismutase (SOD) and catalase (CAT) [16]. TNF-α and IL-8 exert cardiac depression by reducing myocardial shortening [17], further jeopardizing the patient's hemodynamics.…”

Therapeutic effects of curcumin on sepsis and mechanisms of action: A systematic review of preclinical studies

“…Oxidative stress is the serious cause of organ disability in critically ill patients (Ghashut et al, 2017). Reducing the antioxidant capacity (Rogobete et al, 2017) in these patients due to inefficient oxidative phosphorylation (Lorente et al, 2018) leads to an increase in free radicals (Oliveira, Pontes-de-Carvalho, Couto, & Noronha-Dutra, 2017), which is actually a possible mechanism for damage caused by the sepsis (Kumar et al, 2018). Some natural compounds and herbal derivatives have anti-inflammatory and antioxidant effects that can improve sepsis symptoms, such as zinc (Ganatra et al, 2017), magnesium (Esen et al, 2004), Q10 (Abitagaoglu et al, 2015), and curcumin (Chen, Lu, et al, 2018).…”