Evaluation of oxidative stress based on lipid hydroperoxide, vitamin C and vitamin E during apoptosis and necrosis caused by thioacetamide in rat liver

Sun, Fang; Hayami, Shoko; Ogiri, Yukako; Haruna, Sakiko; Tanaka, Kyoko; Yamada, Yasuko; Tokumaru, Sadako; Kojo, Shosuke

doi:10.1016/s0925-4439(99)00100-3

Cited by 82 publications

(66 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present study, the SOD and CAT activities were significantly decreased after D-Gal administration, while the group of rats administered with ULP did not exhibit impairment of antioxidant enzymes (p50.001; Figure 3a and b). This result is in agreement with a general view that administration of an antioxidant agent can neutralize potentially harmful free radicals in the cells before they can cause lipid or protein oxidation (Sun et al, 2000). Few studies have shown that Phase II enzyme genes, which are also referred to as antioxidant response elements, are triggered by oxidant stress or by mixed reactions from other antioxidant products (Cahyana et al, 1992;Wolf, 2001).…”

supporting

confidence: 90%

Sulfated polysaccharide isolated fromUlva lactucaattenuatesd-galactosamine induced DNA fragmentation and necrosis during liver damage in rats

Sathivel

Balavinayagamani

Rao

et al. 2013

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Ulva lactuca Linnaeus (Chlorophyceae), a commonly distributed seaweed, is rich in polysaccharide but has not been studied extensively.Objective: The present study investigated the effects of crude fraction of Ulva lactuca polysaccharide (ULP) on D-galactosamine (D-Gal)-induced DNA damage, hepatic oxidative stress, and necrosis in rats. Materials and methods: The rats were treated with ULP (100 mg/kg, orally) for 4 weeks before a single intraperitoneal injection of D-Gal (500 mg/kg). In addition to liver cell necrosis and DNA damage, antioxidant parameters, such as lipid peroxide (LPO), superoxide dismutase, and catalase, and histopathology of liver tissue were evaluated. Results: ULP pre-treatment significantly attenuated a D-Gal-induced decrease in DNA and RNA levels (3.67 AE 0.38) and (5.42 AE 0.46), respectively. Comet tail length and acridine staining confirmed the number of cells undergoing necrosis were relatively lower in ULP treated rats (30 mm and 8-10% of counted cells) compared to rats treated with D-Gal (60 mm and 16% of counted cells). Biochemical (LPO, SOD and CAT) and histological evaluation (p50.01) confirmed the anti-hepatotoxic and antioxidant property of crude polysaccharide against D-Gal-induced elevation of LPO and infiltration of inflammatory cells into liver tissue. Discussion and conclusion: Although our previous studies have reported on the protective role of ULP against liver toxicity, our present findings show that ULP improved the hepatic antioxidant defense system against D-Gal-induced DNA damage and necrosis in rats.

show abstract

supporting

confidence: 90%

Sulfated polysaccharide isolated fromUlva lactucaattenuatesd-galactosamine induced DNA fragmentation and necrosis during liver damage in rats

Sathivel

Balavinayagamani

Rao

et al. 2013

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…Schizandrae chinensis, a potent anti-oxidant, lowers ALT levels in patients with chronic viral hepatitis [22] . Administration of glutathione to patients with chronic hepatitis significantly decreases GSH-Px activity of catalase (CAT), and increases superoxide dismutase (SOD) activity [23,24] . N-acetyl-cysteine, sodium selenite and vitamin E have also been studied as supplementation to interferon therapy for patients with chronic HCV infection [25] .…”

Section: Discussionmentioning

confidence: 99%

Antioxidant therapy for chronic hepatitis C after failure of interferon: Results of phase II randomized, double-blind placebo controlled clinical trial

Gabbay

Zigmond²,

Pappo³

et al. 2007

WJG

View full text Add to dashboard Cite

AIM:To assess the safety and efficacy of antioxidant therapy for patients with chronic hepatitis C virus (HCV) infection. METHODS:One hundred chronic HCV infection patients failed in interferon treatment were enrolled and randomly assigned to receive combined intravenous and oral antioxidants or placebo, or oral treatment alone. Primary end points were liver enzymes, HCV-RNA levels and histology. RESULTS:Combined oral and intravenous antioxidant therapy was associated with a significant decline in ALT levels in 52% of patients who received antioxidant therapy vs 20% of patients who received placebo (P = 0.05). Histology activity index (HAI) score at the end of treatment was reduced in 48% of patients who received antioxidant therapy vs 26% of patients who received placebo (P = 0.21). HCV-RNA levels decreased by 1-log or more in 28% of patients who received antioxidant therapy vs 12% who received placebo (P = NS). In part Ⅱ of the trial, oral administration of antioxidants was not associated with significant alterations in any of the end points. CONCLUSION:Antioxidant therapy has a mild beneficial effect on the inflammatory response of chronic HCV infection patients who are non-responders to interferon. Combined antiviral and antioxidant therapy may be beneficial for these patients.

show abstract

“…At the same time, celecoxib significantly reduced the oxidative stress in the liver during the second phase of CCl 4 intoxication based on hepatic vitamin C level, which was the most sensitive indicator of oxidative stress during hepatitis caused by chemicals such as CCl 4 , 15) thioacetamide, 19) or D-galatosamine. 20) Although an antioxidant such as a-tocopherol inhibited liver necrosis caused by CCl 4 via direct reduction of oxidative stress, 21) a different mechanism should operate in the inhibition of oxidative stress by celecoxib.…”

Section: Discussionmentioning

confidence: 91%

Effect of Celecoxib, a Selective Cyclooxygenase-2 Inhibitor on Carbon Tetrachloride Intoxication in Rats

Washino

Koga

Kitamura

et al. 2010

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

CCl 4 (0.5 ml/kg as CCl 4 ) was orally administered to rats. Twelve hours after administration of CCl 4 , plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, indicators of liver necrosis, were significantly higher than those in the control group showing that active liver necrosis took place. At the same time the level of liver vitamin C was decreased significantly compared to that in the control group. Oral administration of 100 mg/kg each of celecoxib 3 and 8 h after CCl 4 treatment did not change plasma ALT and AST and liver vitamin C levels 12 h after CCl 4 treatment, but 24 h after CCl 4 treatment, significantly decreased plasma ALT and AST levels and elevated liver vitamin C level. These finding suggested that celecoxib effectively ameliorated the necrotic action and the oxidative stress induced by CCl 4 in the second phase. Although the plasma levels of all ceramide species were significantly increased 24 h after CCl 4 intoxication, treatment with celecoxib significantly reduced the total ceramide concentration in plasma. These results indicated that celecoxib significantly ameliorated the toxicity of CCl 4 in the second phase.

show abstract

Evaluation of oxidative stress based on lipid hydroperoxide, vitamin C and vitamin E during apoptosis and necrosis caused by thioacetamide in rat liver

Cited by 82 publications

References 25 publications

Sulfated polysaccharide isolated fromUlva lactucaattenuatesd-galactosamine induced DNA fragmentation and necrosis during liver damage in rats

Sulfated polysaccharide isolated fromUlva lactucaattenuatesd-galactosamine induced DNA fragmentation and necrosis during liver damage in rats

Antioxidant therapy for chronic hepatitis C after failure of interferon: Results of phase II randomized, double-blind placebo controlled clinical trial

Effect of Celecoxib, a Selective Cyclooxygenase-2 Inhibitor on Carbon Tetrachloride Intoxication in Rats

Contact Info

Product

Resources

About