Evaluation of oxidative stress before and after control of glycemia and after vitamin E supplementation in diabetic patients

Sharma, Anjali; Kharb, Simmi; Chugh, SN; Kakkar, Rajesh; Singh, Gurdeep

doi:10.1016/s0026-0495(00)91117-x

Cited by 99 publications

(61 citation statements)

References 17 publications

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“…Sesame seeds contain abundant amounts of lignans and the most well known are sesamin, sesamol, and sesamolin which have antioxidant activity, through scavenging free radicals and reducing oxidative stress (Wu et al 2009). In another way, sesame may exert an antioxidant influence through its particular composition characterized by the presence of vitamin E that acts as a good antioxidant (Sharma et al 2000). The current findings of reduced oxidative stress of bone and enhanced antioxidant performance in OVX rats fed on SbO and SO, may be due to the osteoprotective effect of phytoestrogen rich oils.…”

Section: Discussionmentioning

confidence: 70%

Osteoprotective effect of soybean and sesame oils in ovariectomized rats via estrogen-like mechanism

2013

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The purpose of the present study was to investigate the osteoprotective effects of soybean oil (SbO) and sesame oil (SO) in ovarictomized (OVX) rats. The results indicated that the OVX rats exhibited a significant decrease in Ca and P level in both serum and bone, the activities of the antioxidant enzymes SOD and CAT and the antioxidant biomarker GSH accompanied with a marked increase in the oxidative stress markers MDA and PC, the inflammatory indices (TNF-a, CRP levels, WBCs counts and ACP activity) in, both, bone and serum. Supplementating the diet of the OVX rats with SbO (15 % w/w) or SO (10 % w/w) for 2 months to resulted in modulation of the alterations in all tested parameters and succeeded to restore minerals, antioxidant enzymes, antioxidant biomarkers, oxidative stress markers, inflammatory indices, and WBCs counts. It could be concluded that the consumption of diets supplemented with SbO or SO might be useful for preventing bone loss caused by estrogen deficiency in ovariectomy status.

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Section: Discussionmentioning

confidence: 70%

Osteoprotective effect of soybean and sesame oils in ovariectomized rats via estrogen-like mechanism

2013

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“…Vitamin E is major lipid soluble chain breaking antioxidant has been associated with reduction in lipid peroxidation, has been shown to block the formation of malondialdehyde, an end product of lipid peroxidation [22]. But serum MDA level is significantly increased after 3 months follow up in group those were taken only hypoglycemic drugs.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Oxidative Stress in Type 2 Diabetes Mellitus and Follow-up Along with Vitamin E Supplementation

2010

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Increased oxidative stress is a widely accepted participant in the development and progression of diabetes and its complications. The present study has been undertaken to evaluate oxidative stress in type 2 diabetes mellitus and effect of vitamin E supplementation on oxidative stress. In all 120 subjects were enrolled in the present study, 40 subjects are age and sex matched controls. Test group comprised of clinically diagnosed (n = 80) type 2 diabetic patients. Biochemical parameters like serum MDA, nitric oxide, superoxide dismutase, erythrocyte reduced glutathione and platelet aggregation were analyzed in control and diabetic group. Test group is further categorized as Group I (n = 40) diabetics were treated by only hypoglycemic drugs and Group II (n = 40) diabetics were treated by hypoglycemic drugs with vitamin E supplementation. All above biochemical parameters were again reassessed after 3 months follow-up in both group and its values were compared with its respective baseline levels. The study shows, reduction of oxidative stress, improvement in antioxidant enzymes and endothelial dysfunction in group II, those were on treatment of hypoglycemic drugs along with vitamin E supplementation. Hence the present study may conclude that vitamin E supplementation along with hypoglycemic drugs may be beneficial to type 2 DM patients to minimize vascular complications.

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“…We have also reported that decreasing intraislet GSH levels by the GCL inhibitor buthionine sulfoximine augments the adverse effects of ROS on beta cell function (6). These observations are clinically relevant because decreases in GSH levels or the ratio of reduced to oxidized glutathione (GSH/GSSG) measured in the red blood cells of type 2 diabetic patients correlate with worsening of the diabetic state (11)(12)(13).…”

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confidence: 89%

Adenoviral Overexpression of the Glutamylcysteine Ligase Catalytic Subunit Protects Pancreatic Islets against Oxidative Stress

Tran

Parker

Leroy

et al. 2004

Journal of Biological Chemistry

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The catalytic subunit of glutamylcysteine ligase (GCLC) primarily regulates de novo synthesis of glutathione (GSH) in mammalian cells and is central to the antioxidant capacity of the cell. However, GCLC expression in pancreatic islets has not been previously examined. We designed experiments to ascertain whether GCLC is normally expressed in islets and whether it is up-regulated by interleukin-1␤ (IL-1␤). GCLC expression levels were intermediate compared with other metabolic tissues (kidney, liver, muscle, fat, and lung). IL-1␤ up-regulated GCLC expression (10 ng/ml IL-1␤, 3.76 ؎ 0.86; 100 ng/ml IL-1␤, 4.22 ؎ 0.68-fold control) via the p38 form of mitogen-activated protein kinase and NF B and also increased reactive oxygen species levels (10 ng/ml IL-1␤, 5.41 ؎ 1.8-fold control). This was accompanied by an increase in intraislet GSH/GSSG ratio (control, 7.1 ؎ 0.1; 10 ng/ml IL-1␤, 8.0 ؎ 0.5; 100 ng/ml IL-1␤, 8.2 ؎ 0.5-fold control; p < 0.05). To determine whether overexpression of GCLC increases the antioxidant capacity of the islet and prevents the adverse effects of IL-1␤ on glucose-induced insulin secretion, islets were infected with an adenovirus encoding GCLC. IL-1␤ significantly decreased glucose-stimulated insulin secretion (control, 123.8 ؎ 17.7; IL-1␤, 40.2 ؎ 3.9 microunits/ml insulin/islet). GCLC overexpression increased intraislet GSH levels and partially prevented the decrease in glucose-stimulated insulin secretion caused by IL-1␤. These data provide the first report of GCLC expression in the islet and demonstrate that adenoviral overexpression of GCLC increases intracellular GSH levels and protects the beta cell from the adverse effects of IL-1␤.Glutamylcysteine ligase (GCL) 1 is the primary and ratelimiting enzyme responsible for de novo synthesis of intracellular glutathione (GSH). This enzyme catalyzes ATP-dependent ligation of L-glutamate and L-cysteine to form ␥-glutamyl-L-cysteine, which undergoes another ATP-dependent ligation with glycine catalyzed by glutathione synthetase to form the final GSH product (1, 2). GCL is a heterodimer, composed of a light regulatory subunit as well as a heavy catalytic subunit. Although both are required for optimal enzyme activity, overexpression of the catalytic subunit GCLC alone is sufficient to increase enzyme activity significantly over control levels (1). The regulation of GCL expression and activity has been studied in many other cell types, including mesangial and endothelial cells, but not the pancreatic islet. Long term exposure to high glucose levels decreases GCL expression in mesangial, retinal Muller, and endothelial cells, which results in a decrease in GSH levels (3-5). Long term exposure to high glucose conditions inhibits endothelial cells from responding to cytokine exposure with an increase in GCL expression and activity (3). That the pathogenesis of diabetes mellitus involves interactions with IL-1␤ and oxidative stress secondary to chronic hyperglycemia (6, 7) raises the questions of whether GCL is normally expressed in the islet and whe...

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Evaluation of oxidative stress before and after control of glycemia and after vitamin E supplementation in diabetic patients

Cited by 99 publications

References 17 publications

Osteoprotective effect of soybean and sesame oils in ovariectomized rats via estrogen-like mechanism

Osteoprotective effect of soybean and sesame oils in ovariectomized rats via estrogen-like mechanism

Evaluation of Oxidative Stress in Type 2 Diabetes Mellitus and Follow-up Along with Vitamin E Supplementation

Adenoviral Overexpression of the Glutamylcysteine Ligase Catalytic Subunit Protects Pancreatic Islets against Oxidative Stress

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