Evaluation of Oxidative Stress in Patients with Difficult-to-Heal Skin Wounds Treated with Hyperbaric Oxygen

Paprocki, J; Pawłowska, Marta; Sutkowy, Paweł; Piechocki, Jacek; Woźniak, Alina

doi:10.1155/2020/1835352

Cited by 11 publications

(4 citation statements)

References 38 publications

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“…The outcome showed a decrease which was significant in MDA between the last session of day 20 and day one. (27) Although using a lower HBO pressure and shorter time per session, as well as a longer duration of therapy, the results are the same as this study which showed that HBO therapy could reduce MDA levels.…”

Section: Discussionsupporting

confidence: 75%

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Hyperbaric Oxygen Ameliorates The Expression of Tumor Growth Factor-β and Malondialdehyde in Pristane-induced Lupus Nephritis Mice Model

Soetjipto

Murbani

Harnanik³

2023

Indones Biomed J

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BACKGROUND: Lupus nephritis (LN) is associated with chronic renal failure and a high mortality rate. Tumor growth factor-β (TGF-β) plays a basic part in keeping up immune homeostasis, meanwhile extensive oxidation of the lipid membrane in LN causes the arrangement of malondialdehyde (MDA). Up to date, the mechanism of hyperbaric oxygen (HBO) therapy in LN has not been elucidated well. Hence this study was conducted to assess the impact of HBO therapy on TGF-β and MDA expression in the pristane-induced LN mice model.METHODS: Thirty-two mice aged 8-12 old weeks were isolated into 4 groups: normal saline-injected mice (group 1); pristane-injected mice (group 2); as well as pristane-injected followed by HBO-exposured with 2.4 (group 3) and 1.5 (group 4) atmosphere absolute (ATA) pressure. Pristane were injected intraperitoneal to initiate LN. Two months after pristane injection, the mice were placed within 24 hours in the special cage for metabolic examination to determined that pristane-induced LN mice model was successfully formed and marked by the occurring proteinuria. Oxidative stress was assessed by examining MDA, while systemic inflammation was assessed by examining by TGF-β. MDA and TGF-β serum were measured by enzyme-linked immunosorbent assay (ELISA).RESULTS: A significantly lower (p<0.050) MDA expression was found in group 3 in comparison with group 2. The results also showed that TGF-β level was significantly increased (p<0.050) in group 3 compared to group 2.CONCLUSION: HBO therapy ameliorates inflammation in LN by diminishing MDA and expanding TGF-β levels through activating antioxidants.KEYWORDS: lupus nephritis, hyperbaric oxygen, malondialdehyde, tumor growth factor-β

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Section: Discussionsupporting

confidence: 75%

“…(26) This treatment increments the expression of diverse growth factors. (27) The use of HBO therapy shows a hormetic effect by inducing reactive oxygen species (ROS) at moderate levels. (28) Moderate concentrations of ROS are useful for cell signaling and the immune system.…”

Section: Introductionmentioning

confidence: 99%

Hyperbaric Oxygen Ameliorates The Expression of Tumor Growth Factor-β and Malondialdehyde in Pristane-induced Lupus Nephritis Mice Model

Soetjipto

Murbani

Harnanik³

2023

Indones Biomed J

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“…Diabetic ulceration is a common diabetic complication, and it causes chronic wound infection that is a serious medical problem that threatens the health and quality of life of diabetic patients [ 3 – 5 ]. ROS are generated during incomplete oxygen metabolism, and under diabetic conditions, immune cells increase ROS levels in the wound microenvironment, leading to stubborn scars and prolonged wounds [ 6 – 8 ]. The superfluous ROS within the impaired wound can promote intense inflammatory reactions to make the wounds fragile, but also restricts skin regeneration by stem cells and macrophages [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…To overcome these challenges, it is crucial to develop effective treatments that can simultaneously target bacterial infections and scavenge excessive ROS, promoting the recovery of diabetic wounds.In cellular enzyme-involved metabolism, oxygen undergoes a series of one-electron reactions, which alternately leads to the formation of several kinds of ROS, including superoxide anion (O 2 •− ), hydroxyl radical (OH·), and hydrogen peroxide (H 2 O 2 ). To defend against excessive ROS, the body has endogenous antioxidases, such as catalase (CAT), SOD, and GPx, are capable of catalyzing the disproportionation reactions of O 2 •− and H 2 O 2 into H 2 O [ 6 , 13 , 14 ]. However, these natural enzymes are usually unstable, with a short half-life in circulation, and are hard to be adequately produced in wound [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Multifunctional nanozyme-reinforced copper-coordination polymer nanoparticles for drug-resistance bacteria extinction and diabetic wound healing

Zhao,

Xu,

Sun

et al. 2023

Biomater Res

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Background Drug-resistant bacterial infections in chronic wounds are a persistent issue, as they are resistant to antibiotics and can cause excessive inflammation due to generation of reactive oxygen species (ROS). An effective solution would be to not only combat bacterial infections but also scavenge ROS to relieve inflammation at the wound site. Scaffolds with antioxidant properties are attractive for their ability to scavenge ROS, and there is medical demand in developing antioxidant enzyme-mimicking nanomaterials for wound healing. Methods In this study, we fabricated copper-coordination polymer nanoparticles (Cu-CPNs) through a self-assembly process. Furthermore, ε-polylysine (EPL), an antibacterial and cationic polymer, was integrated into the Cu-CPNs structure through a simple one-pot self-assembly process without sacrificing the glutathione peroxidase (GPx) and superoxide dismutase (SOD)-mimicking activity of Cu-CPNs. Results The resulting Cu-CPNs exhibit excellent antioxidant propertiesin mimicking the activity of glutathione peroxidase and superoxide dismutase and allowing them to effectively scavenge harmful ROS produced in wound sites. The in vitro experiments showed that the resulting Cu-CPNs@EPL complex have superior antioxidant properties and antibacterial effects. Bacterial metabolic analysis revealed that the complex mainly affects the cell membrane integrity and nucleic acid synthesis that leads to bacterial death. Conclusions The Cu-CPNs@EPL complex has impressive antioxidant properties and antibacterial effects, making it a promising solution for treating drug-resistant bacterial infections in chronic wounds. The complex’s ability to neutralize multiple ROS and reduce ROS-induced inflammation can help relieve inflammation at the wound site. Graphical Abstract Schematic illustration of the ROS scavenging and bacteriostatic function induced by Cu-CPNs@EPL nanozyme in the treatment of MRSA-infected wounds.

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Is there a rationale for hyperbaric oxygen therapy in the patients with Post COVID syndrome?

Pawlik,

Rinneberg,

Koch

et al. 2024

Eur Arch Psychiatry Clin Neurosci

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The SARS-CoV-2 pandemic has resulted in 762 million infections worldwide from 2020 to date, of which approximately ten percent are suffering from the effects after infection in 2019 (COVID-19) [1, 40]. In Germany, it is now assumed that at least one million people suffer from post-COVID condition with long-term consequences. These have been previously reported in diseases like Myalgic Encephalomyelitis (ME) and Chronic Fatigue Syndrome (CFS). Symptoms show a changing variability and recent surveys in the COVID context indicate that 10–30 % of outpatients, 50 to 70% of hospitalised patients suffer from sequelae. Recent data suggest that only 13% of all ill people were completely free of symptoms after recovery [3, 9]. Current hypotheses consider chronic inflammation, mitochondrial dysfunction, latent viral persistence, autoimmunity, changes of the human microbiome or multilocular sequelae in various organ system after infection. Hyperbaric oxygen therapy (HBOT) is applied since 1957 for heart surgery, scuba dive accidents, CO intoxication, air embolisms and infections with anaerobic pathogens. Under hyperbaric pressure, oxygen is physically dissolved in the blood in higher concentrations and reaches levels four times higher than under normobaric oxygen application. Moreover, the alternation of hyperoxia and normoxia induces a variety of processes at the cellular level, which improves oxygen supply in areas of locoregional hypoxia. Numerous target gene effects on new vessel formation, anti-inflammatory and anti-oedematous effects have been demonstrated [74]. The provision of intermittently high, local oxygen concentrations increases repair and regeneration processes and normalises the predominance of hyperinflammation. At present time only one prospective, randomized and placebo-controlled study exists with positive effects on global cognitive function, attention and executive function, psychiatric symptoms and pain interference. In conclusion, up to this date HBO is the only scientifically proven treatment in a prospective randomized controlled trial to be effective for cognitive improvement, regeneration of brain network and improvement of cardiac function. HBOT may have not only theoretical but also potential impact on targets of current pathophysiology of Post COVID condition, which warrants further scientific studies in patients.

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Evaluation of Oxidative Stress in Patients with Difficult-to-Heal Skin Wounds Treated with Hyperbaric Oxygen

Cited by 11 publications

References 38 publications

Hyperbaric Oxygen Ameliorates The Expression of Tumor Growth Factor-β and Malondialdehyde in Pristane-induced Lupus Nephritis Mice Model

Hyperbaric Oxygen Ameliorates The Expression of Tumor Growth Factor-β and Malondialdehyde in Pristane-induced Lupus Nephritis Mice Model

Multifunctional nanozyme-reinforced copper-coordination polymer nanoparticles for drug-resistance bacteria extinction and diabetic wound healing

Is there a rationale for hyperbaric oxygen therapy in the patients with Post COVID syndrome?

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