Evaluation of p-aminohippurate as a marker for blood-flow determinations in cattle

Abstract

Format of Dissertation 2 PART I. DETERMINATION OF PARA-AMINOHIPPURIC ACID AND N-ACETYL-PARA-AMINOHIPPURIC ACID BY HIGH PRESSURE LIQUID CHROMATOGRAPHY 4 Introduction 4 Literature Review 4 Materials and Methods 7 Materials 7 Methods 8 Detection wavelength 8 Chromatographic conditions Detector linearity Deprotelnlzatlon Barium hydroxide-zinc sulfate Perchloric acid-potassium hydroxide Peak-height evaluation Results and Discussion Detection wavelength Chromatographic condlt^on^ Detector linearity Deprotelnlzatlon Barium hydroxide-zinc sulfate Perchloric acid-potassium hydroxide Conclusions PART II. PARA-AMINOHIPPURIC ACID IN VIVO Literature Review Some techniques for the determination of flow Electromagnetic flowmeters Ultrasound flowmeters 30 Thermal methods 31 Other methods 32 General limitations Determination of blood flow by indicator-dilution 34 ill Page Renal transport of PAH PAH clearances and kinetics in vivo Metabolism of PAH Materials and Methods Experiments with non-radioactive PAH and aPAH Single injections Continuous infusions Experiments with radioactive PAH Results and Discussion Experiments with non-radioactive PAH and aPAH Single injection Continuous infusion General comments on the non-radioactive experiments.