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Introduction. Knee Osteoarthritis (OA) is a multifactorial disease resulting from the interaction of many environmental, epigenetic and genetic risk factors, and the latter account for 40% to 65%. Genetic bases of the knee OA based on genome-wide association search (GWAS) are being actively studied by many scientific teams around the world. At the same time, the results obtained are often contradictory and ambiguous, as for the conducted replicative studies of knee OA. This dictates the need for additional replicative studies in various populations, including populations of Russia, which are characterized by significant ethno-territorial variability, in order to identify specific GWAS-significant polymorphic markers of candidate genes associated with OA in these individual populations. The aim of the study was to analyze genome-wide studies of knee OA and to establish GWAS-significant polymorphic loci associated with OA. Materials and methods. The search for publications was carried out in the electronic databases PubMed, PubMedCentral, eLIBRARY, in the GWAS catalog for the period from 2008 to the present by the keywords: knee osteoarthritis, GWAS studies, candidate genes. Results. First, to date, 14 genome-wide studies of knee OA have been performed, as a result of which about 80 GWAS-significant polymorphic loci associated with the risk of knee OA have been identified. Secondly, all GWAS of the knee OA were carried out abroad on samples from various foreign populations, and the samples from the Russian Federation were not included in these studies. Third, only two GWAS-significant polymorphic loci for OA (rs143384 of the GDF5 gene and rs3771501 of the TGFA gene) were replicated at the genome-wide significance level (p5x10-08) in two different studies. Fourth, the data obtained indicate the presence of two regions of chromosomes (6p21.32 and 7q22.3), in which the largest number of GWAS-significant polymorphic loci for OA is located - 3SNPs in each (6p21.32 - rs10947262, rs7775228, rs9277552; 7q22.3 - rs4730250, rs10953541, rs3815148). Fifth, with an increase in the volume of the studied samples of patients and control in genome-wide studies of knee OA, the number of identified GWAS-significant polymorphisms also increases. Conclusion. The main genome-wide studies of knee OA were reviewed and GWAS-significant polymorphisms associated with OA were identified. The obtained materials on GWAS-significant loci can be used both in the selection of polymorphisms in replicative studies of OA in various populations of Russia, and for expanding the understanding of the molecular genetic mechanisms of the disease development.
Introduction. Knee Osteoarthritis (OA) is a multifactorial disease resulting from the interaction of many environmental, epigenetic and genetic risk factors, and the latter account for 40% to 65%. Genetic bases of the knee OA based on genome-wide association search (GWAS) are being actively studied by many scientific teams around the world. At the same time, the results obtained are often contradictory and ambiguous, as for the conducted replicative studies of knee OA. This dictates the need for additional replicative studies in various populations, including populations of Russia, which are characterized by significant ethno-territorial variability, in order to identify specific GWAS-significant polymorphic markers of candidate genes associated with OA in these individual populations. The aim of the study was to analyze genome-wide studies of knee OA and to establish GWAS-significant polymorphic loci associated with OA. Materials and methods. The search for publications was carried out in the electronic databases PubMed, PubMedCentral, eLIBRARY, in the GWAS catalog for the period from 2008 to the present by the keywords: knee osteoarthritis, GWAS studies, candidate genes. Results. First, to date, 14 genome-wide studies of knee OA have been performed, as a result of which about 80 GWAS-significant polymorphic loci associated with the risk of knee OA have been identified. Secondly, all GWAS of the knee OA were carried out abroad on samples from various foreign populations, and the samples from the Russian Federation were not included in these studies. Third, only two GWAS-significant polymorphic loci for OA (rs143384 of the GDF5 gene and rs3771501 of the TGFA gene) were replicated at the genome-wide significance level (p5x10-08) in two different studies. Fourth, the data obtained indicate the presence of two regions of chromosomes (6p21.32 and 7q22.3), in which the largest number of GWAS-significant polymorphic loci for OA is located - 3SNPs in each (6p21.32 - rs10947262, rs7775228, rs9277552; 7q22.3 - rs4730250, rs10953541, rs3815148). Fifth, with an increase in the volume of the studied samples of patients and control in genome-wide studies of knee OA, the number of identified GWAS-significant polymorphisms also increases. Conclusion. The main genome-wide studies of knee OA were reviewed and GWAS-significant polymorphisms associated with OA were identified. The obtained materials on GWAS-significant loci can be used both in the selection of polymorphisms in replicative studies of OA in various populations of Russia, and for expanding the understanding of the molecular genetic mechanisms of the disease development.
The aim. To compare the incidence of knee arthroplasty in patients receiving standard treatment with non-steroidal anti-inflammatory drugs (NSAIDs) in combination with symptomatic slow acting drugs for osteoarthritis (SYSADOA), or combination of NSAIDs and SYSADOA with low-dose radiation therapy (LDRT) in patients with stage 0–2 knee osteoarthritis (OA). Materials and methods. The article presents the results of randomized controlled study of 292 patients with confirmed knee OA according to Altman’s criteria (1991) and Kellgren – Lawrence radiographic stages 0–2 who were randomized into two groups. The control group (n = 146) received standard therapy of NSAIDs + SYSADOA. Patients of the study group (n = 146) received combination of standard therapy and LDRT up to a total dose of 4.5 Gy. The cumulative risk of knee arthroplasty was assessed using actuarial analysis and the Kaplan – Meier method. Attributable (AF) and population attributable (PAF) fractions were calculated to assess LDRT preventive potential. Results. The total observation period was 2131.2 person-years. Knee arthroplasty was performed in 4.1 % (n = 6) of patients in the study group against 7.5 % (n = 11) in the control group. The incidence density ratio was 0.60 (95% CI: 0.18–1.88), which corresponds to a risk reduction by 67 %, but the differences were not statistically significant due to the small number of cases (p = 0.340). The AF was 40 % while the PAF was 21 %. Conclusions. The use of LDRT reduces the risk of knee arthroplasty by two-thirds and has the potential to prevent 21 % cases of knee arthroplasty in patients with knee OA. A study on a larger sample is required.
Up to 25% of patients after knee arthroplasty are not satisfied with the results of the operation. Revision interventions are performed in 60–80% of cases in the first 2–5 years after the primary arthroplasty. Aim. To evaluate the effectiveness of the early postoperative rehabilitation comprehensive program from the standpoint of the International Classification of Functioning (ICF) to improve the results of rehabilitation after arthroplasty. Material and methods. The results of 180 patients rehabilitation after the total knee arthroplasty (TKA) with simultaneous reconstruction of the lower limb biological axis were evaluated in two groups: I – observations (n=120), II – comparisons (n=60). Postoperative rehabilitation in the group I was carried out according to of the early rehabilitation comprehensive program after TKA, developed in the clinic, in the group II – according to the standard scheme. State of the patient was assessed by the dynamics of the pain syndrome, lower limbs muscle strength, goniometric indicators, the severity of lameness, muscle hypotrophy, limb shortening value, functional activity and quality of life. For analysis of changes in the level of damage according to the ICF, clinical tests were used. Results. From the standpoint of the ICF, the results of patients using the early postoperative rehabilitation program were equal or superior to the results of the comparison group. Conclusion. A rehabilitation program for patients after TKA, formed on the basis of a system for assessing the structural, functional and social adaptation characteristics of the patient, is effective and allows to predict the effectiveness of the rehabilitation technologies.
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