Evaluation of Pediococcus pentosaceus strains isolated from Idly batter for probiotic properties in vitro

Vidhyasagar, Venkatasubramanian; Jeevaratnam, Kadirvelu

doi:10.1016/j.jff.2012.10.012

Cited by 130 publications

(92 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All the LAB isolates had shown high auto-aggregation and co-aggregation characteristics. The aggregation between the same LAB isolates aids in colonization thereby preventing pathogen adhesion in the intestinal mucosa [20] and the co-aggregation of LAB and pathogens enables the LAB to find the pathogen in a close proximity and eliminate pathogen by releasing antimicrobial substances [20]. The antimicrobial substances released by aLAB isolate should inhibit the co-aggregating pathogenic microbe partner otherwise both may co-exist and form mixed culture biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of gastric juice on the survival of the isolates was checked at 0, 2 and 4 h because the time from entrance to release from the stomach is 3 -4 h and similarly, for the intestinal juice also the survivability of isolates was checked at 0, 2 and 4 h [20]. The isolates were propagated overnight in MRS broth, cells were harvested by centrifugation and 10 9 -10 10 CFU mL −1 were suspended in artificial gastric fluid (NaCl, 0.72 g•L ; pepsin, 0.3 g•L −1 ) adjusted to pH 2.0, 2.5 and 3.0 and incubated for 0, 2 and 4 h. The intestinal juice resistance was studied by exposing the isolates (10 9 -10 10 CFU mL −1 ) to artificial intestinal fluid (0.1% w/v pancreatin and 0.3% w/v bile salts, pH 8.0) for 0, 2 and 4 h [20] [21].…”

Section: Resistance To Simulated Gastric Fluid and Intestinal Fluidmentioning

confidence: 99%

“…The isolates were propagated overnight in MRS broth, cells were harvested by centrifugation and 10 9 -10 10 CFU mL −1 were suspended in artificial gastric fluid (NaCl, 0.72 g•L ; pepsin, 0.3 g•L −1 ) adjusted to pH 2.0, 2.5 and 3.0 and incubated for 0, 2 and 4 h. The intestinal juice resistance was studied by exposing the isolates (10 9 -10 10 CFU mL −1 ) to artificial intestinal fluid (0.1% w/v pancreatin and 0.3% w/v bile salts, pH 8.0) for 0, 2 and 4 h [20] [21]. Samples from all trials plated in MRS agar to count colony forming units.…”

Section: Resistance To Simulated Gastric Fluid and Intestinal Fluidmentioning

confidence: 99%

“…) and kept for 90 min at 37˚C [20]. After incubation, wells were washed gently with PBS thrice and the non-adherent recovered LAB cells were plated over MRS agar.…”

Section: Lab Adhesion and Inhibition Of Pathogen Adhesion To Cell Linesmentioning

confidence: 99%

“…For the pathogen (Listeria monocytogenes MTCC 657) exclusion assay, the monolayers were first treated with the LAB isolates (5 × 10 10 -10 11 CFU mL −1 ) for 90 min, washed with PBS to remove non-adhered LAB followed by addition of Listeria cells (2 × 10 10 CFU mL −1 ) suspended in serum and antibiotic free medium and incubated for 60 min [20]. The excluded Listeria cells were recovered by washing with the PBS and were plated on Listeria selective agar [28].…”

Section: Lab Adhesion and Inhibition Of Pathogen Adhesion To Cell Linesmentioning

confidence: 99%

See 4 more Smart Citations

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

Dubey¹,

Jeevaratnam²

2015

AiM

Self Cite

View full text Add to dashboard Cite

The in vitro screening of lactobacilli isolates from uttapam batter, fermented supplementing with Piper betle L. leaves, was performed in order to select potent isolates for probiotic use. Their resistance to simulated gastric and intestinal juices as well as their adhesion to epithelial intestinal HCT-15 and vaginal HeLa cell lines were assessed and also evaluated for their immunomodulatory ability in ex vivo condition. Isolates were able to adhere as well as reduce pathogen adhesion to monolayer cell lines' surfaces and reduced production of pro-inflammatory cytokine TNF-α in LPS treated PBMCs, while enhanced that of anti-inflammatory cytokine IL-10. The isolates exhibited properties of auto-aggregation, co-aggregation, hydrophobicity, bile salt hydrolase activity and strong antimicrobial activity against pathogenic bacteria. Thus, the different lactobacilli isolates displayed potent probiotic and immunomodulatory properties among which AJ7 and AJ82 had a great potential and may have applications in fermented foods as immunomodulatory probiotic additives.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Resistance To Simulated Gastric Fluid and Intestinal Fluidmentioning

confidence: 99%

Section: Resistance To Simulated Gastric Fluid and Intestinal Fluidmentioning

confidence: 99%

“…) and kept for 90 min at 37˚C [20]. After incubation, wells were washed gently with PBS thrice and the non-adherent recovered LAB cells were plated over MRS agar.…”

Section: Lab Adhesion and Inhibition Of Pathogen Adhesion To Cell Linesmentioning

confidence: 99%

Section: Lab Adhesion and Inhibition Of Pathogen Adhesion To Cell Linesmentioning

confidence: 99%

See 3 more Smart Citations

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

Dubey¹,

Jeevaratnam²

2015

AiM

Self Cite

View full text Add to dashboard Cite

show abstract

Biosorption of cadmium by potential probiotic Pediococcus pentosaceus using in vitro digestion model

Yang

2019

Biotech and App Biochem

View full text Add to dashboard Cite

An exponential increase of heavy metal toxicant in the human body is a growing health-related problem. In the present study, Pediococcus pentosaceus FB145 and FB181 strains were isolated from fermented seafood and served as highly Cd-resistant strains. The unchanged structural cells of P. pentosaceus strains after treatment with Cd cations were investigated using a scanning electron microscope energy dispersive X-ray analysis. Furthermore, both P. pentosaceus strains showed strong human gastrointestinal tract resistance properties. The Cd biosorption results fit the pseudo-second-order kinetics with capacities for P. pentosaceus FB145 and FB181 for Cd were 52.8 and 50.3 mg/g, respectively, whereas the maximum biosorption capacities were 52.65 and 50.35 mg/g, respectively. The equilibrium data were well fitted to the Freundlich isotherm model. The binding of Cd to bacterial cells may be caused by the presence of different functional groups such as carboxyl, amide, and phosphate on cell surface, which was confirmed by the Fourier transform infrared-attenuated total reflectance spectra. Moreover, these strains decreased the Cd bioaccessibility by 44.7-46.8% in the in vitro digestion model. These findings indicate that P. pentosaceus FB145 and FB181 are novel potent biosorbent for preventing cadmium toxicity and reducing its absorption into the human body.

show abstract

Bacteriocin activity against various pathogens produced by Pediococcus pentosaceus VJ13 isolated from Idly batter

Vidhyasagar

Jeevaratnam

2013

Biomedical Chromatography

Self Cite

View full text Add to dashboard Cite

Bacteriocins, an antimicrobial peptide, is known to have wide spectrum antimicrobial activity against various pathogens. Because they are easily digested in the intestine, they are considered as safe and are widely used as food preservatives. Hence their purification and characterization have attracted considerable attraction, especially for those having activity against human pathogens. In this study, the bacteriocin produced by Pediococcus pentosaceus VJ13 was precipitated with cold acetone and purified by gel permeation chromatography and hydrophobic interaction chromatography. The bacteriocin exhibited antimicrobial activity against various pathogens, like Mycobacterium smegmatis, Klebsiella pneumonia, Clostridium perfringens and Staphylococcus epidermidis. The activity of bacteriocin was lost completely after treatment with protease, which revealed its proteinaceous nature. The bacteriocin was stable up to 100°C and exhibited antilisterial property which is a characteristic feature of class IIa bacteriocins. It was active within the pH range of 2-8 and stable against various chemicals and denaturants. Tricine SDS-PAGE revealed its molecular weight to be 4.0 kDa, where the corresponding activity against Listeria monocytogenes was also noted. Treatment of L. monocytogenes with bacteriocin decreased the viable cell count, and scanning electron microscope analysis revealed membrane pore formation that resulted in the release of intracellular content, suggesting its bactericidal effect.

show abstract

Evaluation of Pediococcus pentosaceus strains isolated from Idly batter for probiotic properties in vitro

Cited by 130 publications

References 45 publications

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

Biosorption of cadmium by potential probiotic Pediococcus pentosaceus using in vitro digestion model

Bacteriocin activity against various pathogens produced by Pediococcus pentosaceus VJ13 isolated from Idly batter

Contact Info

Product

Resources

About

Evaluation of Pediococcus pentosaceus strains isolated from Idly batter for probiotic properties in vitro

Cited by 130 publications

References 45 publications

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with &lt;i&gt;Piper betle&lt;/i&gt; L. Leaves

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with &lt;i&gt;Piper betle&lt;/i&gt; L. Leaves

Biosorption of cadmium by potential probiotic Pediococcus pentosaceus using in vitro digestion model

Bacteriocin activity against various pathogens produced by Pediococcus pentosaceus VJ13 isolated from Idly batter

Contact Info

Product

Resources

About

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves