Evaluation of peptone glucose fluconazole agar as a selective medium for rapid and enhanced isolation ofAspergillus fumigatusfrom the respiratory tract of bronchopulmonary aspergillosis patients colonized byCandida albicans

Abstract

We have reported earlier that Aspergillus fumigatus is inhibited in vitro by Candida albicans which also interferes in its isolation from sputum experimentally seeded with predetermined graded inocula of the two fungi. It was further shown that this interference was neutralized by employing peptone glucose agar with incorporation of fluconazole which is more inhibitory to C. albicans than to A. fumigatus. This communication embodies the results of evaluation of peptone glucose fluconazole agar (PGFA) as a selective culture medium for rapid and enhanced isolation of A. fumigatus from sputum of patients clinically suspected of aspergillosis with C. albicans colonization in the respiratory tract. Of the 23 sputum specimens and one broncho-alveolar lavage collected from 15 suspected aspergillosis patients, A. fumigatus was isolated from all (100%) on PGFA as against only 19 specimens (79%) that proved to be positive on the control PGA medium (P<0.05). The greater efficacy of PGFA than that of PGA was further evident from the 2-fold higher A. fumigatus mean colony count (8.2+/-1.87) on the former medium than on the latter (3.7+/-1.00), and this difference was found to be statistically significant (P<0.05). Besides, A. fumigatus colonies were macroscopically recognizable within 2-3 days on PGFA at 28 degrees C in strong contrast to 5-7 days required on PGA. Based upon these observations, PGFA is recommended for wider application as a selective medium for rapid and enhanced recovery of A. fumigatus from sputum of patients clinically suspected of aspergillosis with C. albicans colonization in their respiratory tract.

Routine processing procedures for isolating filamentous fungi from respiratory sputum samples may underestimate fungal prevalence

“…In all cases the fungi readily grew on both media, but a higher yield of conidia was obtained on PDA. We added antibiotics to the PDA at optimum concentrations for isolation of pathogenic fungi [14], and fluconazole to enhance recovery of A. fumigatus through suppression of Candida [15]. It is unclear as to whether A. fumigatus grows preferentially on PDA compared to SDA or the higher concentration of chloramphenicol in SC plates inhibits A. fumigatus development or the fluconazole enhances A. fumigatus recovery.…”

The Inhibitory Effect of Validamycin A onAspergillus flavus

“…A number of conidia were counted from the filtrate using a hemocytometer. Then, 10 3 conidia were inoculated into culture media [ 39 ], i.e., glucose peptone agar (peptone 10 g, glucose 20 g, agar 20 g, distilled water 1000 ml, and pH 6.8–7.0), trehalose peptone agar (peptone 10 g, trehalose 10 g, agar 20 g, distilled water 1000 ml, and pH 6.8–7.0), and peptone agar (peptone 10 g, agar 20 g, distilled water 1000 ml, and pH 6.8–7.0), incubated at 37°C for 2–5 days. The radial fungal growth was measured in three biological replicates.…”

Teknik Isolasi Senyawa Bioaktif Dari Kapang Yang Berasal Dari Lingkungan Laut

“…Isolasi kapang dilakukan dengan menempatkan sampel pada media selektif untuk kapang. Media yang dapat digunakan untuk isolasi kapang ada berbagai jenis yaitu potato dextrose agar (PDA) (Suryono et al, 2002;Nugroho et al, 2003), peptone glucose fluconazole agar (PGFA) (Randhawa et al, 2005), potato carot agar, pentachloronitrobenzene agar, rose bengal medium, yeast malt agar (Demain & Solomon, 2986), yeast peptone glucose (YPG) Li et al, 2006;Rahman et al, 2006dan Zhang & Son, 2006Ngunyen & Son, 2007;Zhang et al, 2008), dan media lainnya. Namun dari sekian banyak media yang dapat digunakan untuk isolasi kapang, yang akan dijelaskan di sini hanya media YPG.…”