Evaluation of perforated demineralized dentin scaffold on bone regeneration in critical‐size sheep iliac defects

Kabir, Md. Arafat; Murata, Masaru; Akazawa, Toshiyuki; Kusano, Kanichi; Yamada, Kei; Ito, Manabu

doi:10.1111/clr.13000

Cited by 29 publications

(43 citation statements)

References 46 publications

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“…Dentin matrix was demineralized by 0.34 N HNO 3 for 30 mins, and the HNO 3 -DDM was grafted into critical-sized defect in the iliac crest of sheep. New bone formation was observed around DDM, and more bone volume was detected after 4 months than that at 2 months [41]. In another study, dentin matrix was treated with 2% HNO 3 for 10 min, then transplanted into the tooth extraction pocket, and bone augmentation was observed [42].…”

Section: Ddm Preparationmentioning

confidence: 95%

“…The demineralizing time ranged from 30 min to 24 h [38][39][40]. HNO 3 was also used in demineralization [17,41,42]. Dentin matrix was demineralized by 0.34 N HNO 3 for 30 mins, and the HNO 3 -DDM was grafted into critical-sized defect in the iliac crest of sheep.…”

Section: Ddm Preparationmentioning

confidence: 99%

“…Besides DDM particles, DDM blocks were also investigated [14,18,23,41,46]. In one study, DDM blocks with macropores were implanted into critical-sized iliac defects and new bone formation around the DDM had good union with the native bone [41]. Another study evaluated the characteristics of dentin blocks with different demineralization time (demineralized for 10 min, DDB10; for 60 min, DDB60), and assessed new bone formation after grafting the dentin blocks in rat calvaria defects.…”

Section: Ddm Preparationmentioning

confidence: 99%

“…Extensive studies have been conducted on DDM with its osteoinductivity and osteoconductivity in vitro and in vivo [17,18,[48][49][50][51]. In animal studies, DDM enhanced the bone healing process of tooth sockets, and promoted new bone formation in rat calvaria defects, rabbit parietal defects, minipig crania defects, sheep iliac defects, and porcine sinus [17,18,41,[47][48][49]52]. Besides bone, DDM was employed in osteochondral regeneration [20].…”

Section: Application Of Ddm In Bone Regenerationmentioning

confidence: 99%

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Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

et al. 2019

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Demineralized dentin matrix (DDM), derived from human teeth, is an excellent scaffold material with exciting bioactive properties to enhance bone and dental tissue engineering efficacy. In this article, first the nano-structure and bioactive components of the dentin matrix were reviewed. Then the preparation methods of DDM and the resulting properties were discussed. Next, the efficacy of DDM as a bone substitute with in vitro and in vivo properties were analyzed. In addition, the applications of DDM in tooth regeneration with promising results were described, and the drawbacks and future research needs were also discussed. With the extraction of growth factors from DDM and the nano-structural properties of DDM, previous studies also broadened the use of DDM as a bioactive carrier for growth factor delivery. In addition, due to its excellent physical and biological properties, DDM was also investigated for incorporation into other biomaterials design and fabrication, yielding great enhancements in hard tissue regeneration efficacy.

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Section: Ddm Preparationmentioning

confidence: 95%

Section: Ddm Preparationmentioning

confidence: 99%

Section: Ddm Preparationmentioning

confidence: 99%

Section: Application Of Ddm In Bone Regenerationmentioning

confidence: 99%

See 2 more Smart Citations

Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

et al. 2019

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“…Recently, absorbable materials have been needed in the field of tissue engineering. We have been challenging to develop bioabsorbable dentin scaffolds [1][2][3][4][5][6][7][8][9][10][11][12][13], harmonized with bone remodeling, by using the ultrasonic acid-etching technology [14][15][16][17] (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Dentin Materials as Biological Scaffolds for Tissue Engineering

Murata¹,

Okubo²,

Shakya³

et al. 2019

Biomaterial-Supported Tissue Reconstruction or Regeneration

Self Cite

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Vital tooth-derived demineralized dentin matrix (DDM) has a bone-inductive ability, while non-vital tooth-derived DDM lost it. Acid treatment for dentin provides the increase of surface area, the release of matrix-binding growth factors such as BMPs, and the decrease of the infection risk. Human autograft of vital toothderived DDM was achieved first in Japan 2002, while first bone autograft was noted in Italy 1820. This paper introduced dentin/bone biology and a unique clinical case, combined with two types of non-vital tooth-derived DDM (roots, granules) for lateral bone augmentation. A 63-year-old woman revealed highly atrophic mandible in 2015. Three non-vital teeth were extracted, changed in shape, demineralized in 2% HNO 3 , were rinsed, and were grafted immediately. The CT images at 3 months after the graft showed remarkable lateral augmentation. DDM scaffolds were received to host, and two fixtures were placed into the DDM-augmented bone. The patient was successfully restored with their own DDM scaffolds and implant surgery.

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Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration

Peng

Liu

et al. 2023

Adv Healthcare Materials

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Dentin is a natural extracellular matrix, but its availability in bone grafting and tissue engineering applications is underestimated due to a lack of proper treatment. In this study, the concept of extrafibrillar demineralization is introduced into the construction of dentin-derived biomaterials for bone regeneration for the first time. Calcium chelating agents with large molecular weights are used to selectively remove the extrafibrillar apatite minerals without disturbing the intrafibrillar minerals within dentin collagen, resulting in the formation of an extrafibrillarly demineralized dentin matrix (EDM). EDM with distinctive nanotopography and bone-like mechanical properties is found to significantly promote cell adhesion, migration, and osteogenic differentiation in vitro while enhancing in vivo bone healing of rat calvarial defects. The outstanding osteogenic performance of EDM is further confirmed to be related to the activation of the focal adhesion-cytoskeleton-nucleus mechanotransduction axis. Overall, this study shows that extrafibrillar demineralization of dentin has great potential to produce hierarchical collagen-based scaffolds for bone regeneration, and this facile top-down fabrication method brings about new ideas for the biomedical application of naturally derived bioactive materials.

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Evaluation of perforated demineralized dentin scaffold on bone regeneration in critical‐size sheep iliac defects

Abstract: Altogether, these results demonstrated that the human tooth-derived graft material with a unique geometric structure, PR-DDM, contributed to active bone ingrowth in critical-size bone defects. This novel scaffold may have great utility in the near-future clinical application.

Cited by 29 publications

References 46 publications

Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

Dentin Materials as Biological Scaffolds for Tissue Engineering

Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration

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