Evaluation of periimplant bone neoformation using different scanning electron microscope methods for measuring BIC. A dog study.

Abstract

Objetives: The aim of this study was to determine which of three methods for measuring BIC (bone-to-implant contact), using vestibular and lingual scanning electron microscopy (SEM) for different implant systems at 15, 30 and 90 days post-surgery was the most precise. An elemental analysis with SEM was used to evaluate neoformed bone composition for three implant systems at the same study times. Material and Methods: 36 implants were placed in eighteen Beagle dogs mandible about one year old and weighing approximately 12-13 kg in order to evaluate bone apposition to three different implant surfaces. It was used the third and fourth premolar and first molar distal sockets in both quadrants of the mandible (3P3, 4P4 and 1M1). Teeth were hemi-sected and the distal roots were removed. The specimens were prepared for histological examination and each section surface was stained using Masson’s trichrome and hematoxylin and eosin stains. BIC evaluations were performed by the three methods, BIC I (the quantity of mineralized bone in direct contact with the implant’s titanium surface across the entire threaded area); BIC II (along a line that passes from apex to apex of the implant threads); BIC III (both in areas around and above the threads and in between threads). Results: Both BIC and bone content were analyzed for all implants placed in P3, P4 y M1 alveoli on both, the buccal and palatine sides (elemental analysis quantified Ca, P, O and C). It was seen it was only at the ninety-day mark that high percentages of calcium were present. Conclusions: This study suggest that BIC III evaluation is the most certain method for establishing the quantity of bone formed as the BIC area. Key words:Bone-to-impant contact, dogs, extraction socket, implants.

Bone remodeling at implants with different configurations and placed immediately at different depth into extraction sockets. Experimental study in dogs

“…Bone‐to‐implant contact in each histological section was calculated by measuring the length of the implant surface in contact with bone tissue, compared with the total length of the implant surface, and expressed as a percentage. BIC percentages were calculated around the entire implant body, from the first point of BIC, at the most coronal point, evaluating mineralized bone in contact with the implant surface linearly (Calvo‐Guirado et al ). Histomorphometric analysis was performed at ×10 magnification.…”

Histological and Histomorphometric Evaluation of Zirconia Dental Implants Modified by Femtosecond Laser versus Titanium Implants: An Experimental Study in Fox Hound Dogs

“…To do this, the percentage of mineralized bone in direct contact with the titanium/zirconia surface was determined by counting inside the threaded zone. BIC percentages were calculated around the entire implant perimeter from the first point of BIC at the most coronal point, evaluating mineralized bone in contact with the implant surface linearly . Histomorphometric analysis was performed using a video camera (Sony 3CCD, Sony, Berlin, Germany) with ×10 magnification.…”

“…BIC percentages were calculated around the entire implant perimeter from the first point of BIC at the most coronal point, evaluating mineralized bone in contact with the implant surface linearly. 27 Histomorphometric analysis was performed using a video camera (Sony 3CCD, Sony, Berlin, Germany) with ×10 magnification. Images were digitalized (Axiophot-System, Zeiss, Oberkochen, Germany), stored, and reference points were plotted (crestal bone level [CBL]; implant length [IL]; soft tissue [ST]) ( Figure 3).…”

Histological, radiological and histomorphometric evaluation of immediate vs. non‐immediate loading of a zirconia implant with surface treatment in a dog model

“…There are three different ways to measure BIC: (1) BIC I is the amount of mineralized bone in direct contact with the surface of the implant through the entire area of the thread; (2) BIC II measures bone in contact with the implant along a line from vertex to vertex of the implant threads, this measure is therefore considered the measurement of contact between threads and tends to measure both the old bone and some new bone; (3) BIC III measures both in the areas around, above and between the threads (Calvo‐Guirado et al. ). In this study, we used the measurement of BIC III.…”