Evaluation of phenolic acids and phenylpropanoids in the crude drugs

Ivanauskas, Liudas; Jakštas, Valdas; Radušienė, Jolita; Lukošius, Audronis; Baranauskas, Algirdas

doi:10.3390/medicina44010008

Cited by 21 publications

(12 citation statements)

References 8 publications

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“…No external interference with the accuracy and separation was identified when analyzing skin specimens. The results of the analysis supported published data [15, 21], confirming that coumaric, ferulic acids, and vanillin are the dominating components in raw Lithuanian propolis (Table 2). Aqueous propolis extract was used as an active substance's form, for development of hydrophilic gel systems for further studies (Table 1).…”

Section: Resultssupporting

confidence: 86%

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Release of Propolis Phenolic Acids from Semisolid Formulations and Their Penetration into the Human SkinIn Vitro

Žilius

Ramanauskienė

Briedis

2013

Evidence-Based Complementary and Alternative Medicine

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Antioxidant and free radical scavenging effects are attributed to phenolic compounds present in propolis, and when delivered to the skin surface and following penetration into epidermis and dermis, they can contribute to skin protection from damaging action of free radicals that are formed under UV and premature skin aging. This study was designed to determine the penetration of phenolic acids and vanillin into the human skin in vitro from experimentally designed vehicles. Results of the study demonstrated the ability of propolis phenolic acids (vanillic, coumaric, caffeic, and ferulic acids) and vanillin to penetrate into skin epidermis and dermis. The rate of penetration and distribution is affected both by physicochemical characteristics of active substances and physical structure and chemical composition of semisolid vehicle. Vanillin and vanillic acid demonstrated relatively high penetration through epidermis into dermis where these compounds were concentrated, coumaric and ferulic acids were uniformly distributed between epidermis and dermis, and caffeic acid slowly penetrated into epidermis and was not determined in dermis. Further studies are deemed relevant for the development of semisolid topically applied systems designed for efficient delivery of propolis antioxidants into the skin.

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Section: Resultssupporting

confidence: 86%

“…The specificity and identification of phenolic acids were confirmed with a PDA detector and UV spectra of the appropriate standards in the wavelength range of 210–600 nm. The integration of phenolic acids peaks was performed at 290 nm [15]. …”

Section: Methodsmentioning

confidence: 99%

Release of Propolis Phenolic Acids from Semisolid Formulations and Their Penetration into the Human SkinIn Vitro

Žilius

Ramanauskienė

Briedis

2013

Evidence-Based Complementary and Alternative Medicine

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show abstract

“…The eluted components were identified based on the retention time by comparison with the retention time of the reference standard. The identity of constituents was also confirmed with a PDA detector by comparison with UV spectra of the reference standard in the wavelength range of 190-400 nm (15).…”

Section: Methodsmentioning

confidence: 96%

Analysis of content of phenolic acids in Lithuanian propolis using high-performance liquid chromatography technique

Ramanauskienė¹,

Savickas²,

Inkėnienė³

et al. 2009

Medicina

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The aim of the study was to analyze phenolic acids in Lithuanian propolis and to compare it with the composition of propolis in neighboring countries (Latvia and Poland) according to the predominant flora in the collecting places. The study was also aimed at the evaluation of the effect of the layer thickness (mm) of the harvested propolis on the quality of the raw material in determining the amount of phenolic acids.Materials and methods. The object of the study was propolis collected in Lithuania, Poland, and Latvia in late July of 2006 and 2007. The qualitative and quantitative analysis of phenolic acids was performed using the high-performance liquid chromatography technique (HPLC).Results. The results of the study showed that the quantitative and qualitative composition of phenolic acids in propolis depended on the plants from which the bees in the area collected substances for the raw material of propolis. The predominant phenolic acids were determined to be ferulic and coumaric acids, and they may be among the main indicators of quality in the standardization of the raw material and preparations of propolis.Conclusion. We created an HPLC-based analysis method for the identification and quantification of phenolic acids in propolis. The variety of phenolic acids in propolis depends on the vegetation predominating in the harvesting area. Studies have shown that the highest amount of phenolic acids is observed in propolis harvested in areas characterized by the predominance of deciduous trees and meadows. Results have also shown that ferulic and coumaric acids are the predominant phenolic acids in propolis. The thickness of the layer of the collected propolis in the hive also influences its chemical composition.

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“…The mobile phase consisted of solvent A, 0.5% acetic acid; solvent B methanol. The gradient program applied was: 0 min, 90% A; 26.15 min, 60% A; 30 min, 90% A [23]. Detection was carried out at 290 nm and identification and quantification were carried out by comparing with standards (Sigma Aldrich, Bangalore).…”

Section: Hplc Analysismentioning

confidence: 99%

Vir gene inducers in Dunaliella salina ; an insight in to the Agrobacterium -mediated genetic transformation of microalgae

et al. 2015

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Evaluation of phenolic acids and phenylpropanoids in the crude drugs

Abstract: Summary. Phenolic acids and phenylpropanoids have an important biological activity and

Cited by 21 publications

References 8 publications

Release of Propolis Phenolic Acids from Semisolid Formulations and Their Penetration into the Human SkinIn Vitro

Release of Propolis Phenolic Acids from Semisolid Formulations and Their Penetration into the Human SkinIn Vitro

Analysis of content of phenolic acids in Lithuanian propolis using high-performance liquid chromatography technique

Vir gene inducers in Dunaliella salina ; an insight in to the Agrobacterium -mediated genetic transformation of microalgae

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