Evaluation of Phenolic Content, Antioxidant Activity and Cytotoxic Effects of Ulva lactuca and Hypnea Musiformis Marine Algae on MDA-MB-468 Cell Line

Moulazadeh, Alireza; Ranjbar, Razieh; Ardestani, Amin; Keshavarzi, Aref; Karimzadeh, F.; Rahnavard, Maryam; Jeliani, Zahra Zarei; Najafipour, Sohrab

doi:10.18502/jabs.v11i3.8785

Cited by 2 publications

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“…The absorbance of the samples was measured at 765 nm using a Synergy HTX multi-mode reader. Gallic acid served as the standard, and the phenolic content of the extracts was expressed in micrograms of Gallic Acid Equivalent (GAE) per milligram (µgGAE/mg) (10,11). The results were presented as mean ± SD, and all measurements were conducted three times in duplicate.…”

Section: The Phenolic Content Assaysmentioning

confidence: 99%

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Phytochemical and Cytotoxic Properties of Gum and Leaf Extracts of Ferula assafoetida in Breast Cancer

Alireza,

Razieh,

Amin

et al. 2023

JABS

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Background & Objectives: Breast cancer, a significant threat to women's health, is associated with a high annual mortality rate. Oxidative stress, characterized by the accumulation of reactive oxygen species, plays a pivotal role in cancer development. Ferula assafoetida, a traditional medicinal plant, has been widely used in cancer treatment. This study aims to compare the cytotoxic effects, phytochemical profiles, and antioxidant activities of Ferula assafoetida gum (FAGE) and leaf (FALE) extracts. Materials & Methods: The herbal extracts from FAGE and FALE were prepared using the maceration method in 70% v/v ethanol. The phenolic content and radical scavenging activity of the extracts were assessed using the Folin-Ciocalteu and diphenylpicrylhydrazyl (DPPH) assays, respectively. The cytotoxic effects of the extracts were examined on the MDA-MB-231 and MCF-7 cell lines through the MTT assay. Additionally, the morphological changes of the cell lines were observed using an inverted microscope. Results: The phenolic content in FAGE (142.25± 19.35 µgGAE/mg) was significantly higher (P=0.01) than that in FALE (48.15± 3.01 µgGAE/mg). Additionally, the radical scavenging activity and cytotoxic effects of FAGE were also significantly stronger than those of FALE (P< 0.05). Regarding the cytotoxic effects of the extracts, FAGE exhibited IC50 values of 80.59± 10.98 and 59.39± 15.89 µg/mL for MCF-7 and MDA-MB-231 cells, respectively, after 72 hours of incubation. Conclusions: FAGE exhibited a greater cytotoxic effect on the MDA-MB-231 cell line than on the MCF-7, possibly following an estrogen-independent pattern. It appears that FAGE holds significant potential in refining oxidative stress, thereby inhibiting cell proliferation.

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Section: The Phenolic Content Assaysmentioning

confidence: 99%

“…The MTT assay was employed to evaluate the cell viability of MDA-MB-231 and MCF-7 breast cancer cell lines following exposure to FAGE and FALE. Based on a previous study, 10,000 cells were seeded per well in 150 µL of medium and incubated to form a confluent cell population (14,15).…”

Section: Cell Cytotoxicity Of Fage and Fale And Morphological Changesmentioning

confidence: 99%

Phytochemical and Cytotoxic Properties of Gum and Leaf Extracts of Ferula assafoetida in Breast Cancer

Alireza,

Razieh,

Amin

et al. 2023

JABS

View full text Add to dashboard Cite

show abstract

“…The ferric reducing antioxidant power (FRAP) assay was used for the evaluation of monovalent reducing power of TAEO and FAEO according to the previous study [8]. FeSO 4 serial dilutions were also used as the standard, and the antioxidant activity of the essential oils was reported in μmolFe 2+ /g.…”

Section: Monovalent Reducing Powermentioning

confidence: 99%

Cytotoxic effects of Trachyspermum ammi and Ferula assafoetida on MCF-7 and MDA-MB-468 breast cancer cell lines

Moulazadeh

Ranjbar

Ardestani

et al. 2022

Beni-Suef Univ J Basic Appl Sci

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Background The induction of oxidative stress is one of the most important cancer etiologies. Plant essential oils contain many effective antioxidant compounds in improving oxidative stress. In the present study, the pharmacological potential of Trachyspermum ammi essential oil (TAEO) and Ferula assafoetida essential oil (FAEO) was compared in oxidative stress improvement and cytotoxic effect. TAEO and FAEO were prepared by Clevenger apparatus, and the medicinal compounds in the essential oils were evaluated by GC–MS assay. The TAEO and FAEO were also evaluated as to their phenolic and flavonoid content, monovalent reducing power, and total radical scavenging activity, respectively, by Folin–Ciocalteu, aluminum chloride, FRAP, and DPPH methods. The cytotoxic effect of the TAEO and FAEO was evaluated by MTT assay on MCF-7 (ER+) and MDA-MB-468 (ER−) breast cancer cell lines. Results The GC–MS analysis indicated that thymol and (E)-Sec-Butyl propenyl disulfide, respectively, were the highest components of TAEO and FAEO. The phenolic content (P < 0.0001), flavonoid content (P < 0.0001), reducing power (P < 0.0001), radical scavenging activity (P < 0.0001), and cytotoxic effect (P < 0.05) of TAEO were significantly higher than FAEO. The IC50 value of the cytotoxic effect of TAEO on MCF-7 and MDA-MB-468 after 72 h of incubation was, respectively, 192.5 ± 42.57 and 331.4 ± 7.24 ppm. Conclusion The cytotoxic effect of TAEO was more potent on the MCF-7 cell line, probably in an estrogen-dependent manner of cellular growth inhibition. It appears that TAEO has a high capacity for improving oxidative stress and inhibiting cell proliferation in breast cancer.

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Evaluation of Phenolic Content, Antioxidant Activity and Cytotoxic Effects of Ulva lactuca and Hypnea Musiformis Marine Algae on MDA-MB-468 Cell Line

Cited by 2 publications

References 0 publications

Phytochemical and Cytotoxic Properties of Gum and Leaf Extracts of Ferula assafoetida in Breast Cancer

Phytochemical and Cytotoxic Properties of Gum and Leaf Extracts of Ferula assafoetida in Breast Cancer

Cytotoxic effects of Trachyspermum ammi and Ferula assafoetida on MCF-7 and MDA-MB-468 breast cancer cell lines

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