Evaluation of Phosphate Removal from Water by Immobilized Phosphate-Binding Protein PstS.

Abstract

The phosphate (P(i))-binding protein PstS is a member of a family of periplasmic proteins that act as high-affinity receptors for active transport systems in bacteria. PstS protein purified from Pseudomonas aeruginosa was immobilized to N-hydroxysuccinimide-activated Sepharose, packed into a plastic column (5 x 70 mm), and examined for its potential ability to remove P(i) from water. The PstS-Sepharose column completely removed P(i) from 32P-labeled pond water containing about 0.5 microM P(i) (0.015 mg P per liter). More than 90% of 32P-P(i) that was retained in the column could be eluted by washing with low-pH water.

Biological Phosphorus Recovery: Review of Current Progress and Future Needs

“…Li et al (2009) immobilized PBP proteins on bacterial surfaces and observed absolute biosorption of 60 to 80 mg/L total phosphate over 5 hours. Extracellular protein studies were also performed by Kuroda et al (2000), who purified PBP proteins and immobilized them on inert beads to test phosphorus removal in small-scale columns treating ultralow phosphorus pond water. They found that phosphorus could be completely removed, and also eluted back off the column.…”