Borehole water is commonly believed to be pure and safe to drink. The present study assessed the quality of ten selected borehole locations within Ado -Ekiti. This was carried out by assessing the physicochemical parameters, microbial and heavy metal contamination using standard procedures. The physicochemical parameter results showed that Temperature range between 25.99 oC - 26.60 oC, Electrical conductivity range between 41.62 µs/cm - 202.54 µs/cm, pH range between 6.81-7.57, Total dissolved solids range between 28.15 mg/L - 116.22 mg/L, Total suspended solids range between 0.09 mgl -1.27 mgl, Turbidity range between 0.12 NTU-0.71 NTU, Total Hardness range between 29.64 mg/l -80.61 mg/l, Total Alkalinity range between 20.97 mg/l - 65.42 mg/l and all values were within World Health Organisation (WHO) permissible limits. The bacteria count of borehole water sample range from 10.66 ± 0.03 Cfu/ml to 12.56 ± 0.03 Cfu/ml. Highest fungi count of the borehole water sample was in the value range from 8.27 ± 0.00 Sfu/ml to 9.63 ± 0.02 Sfu/ml. The highest coliform detected in the borehole sample range from 5.33 ± 0.58 Cfu/ml to 8.67 ± 8.56 Cfu/ml. The bacteria, fungi and coliform count result observed in this study was higher than the WHO recommended limits. The metals occurred in the samples in this order Cr >Fe > Pb > As > Cd. The heavy metals were present in quantities higher than WHO recommended limit in some of the samples which is an indication of potential health hazards through the water consumption.