Kenya experiences high postharvest losses in the fruit and vegetable subsector, which have been estimated at 50 percent. These losses are mainly due to poor postharvest handling practices, a lack of storage facilities, and/or poor storage conditions. Papaya (Carica papaya L.) is a highly perishable tropical fruit with a short shelf life and requires proper storage and handling practices due to its delicate skin texture, high moisture content, and high respiratory rates. In this study, F hybrid and Mountain variety papaya fruits were collected at Meru County in May 2022 and stored at three different temperatures (6, 16 and 24 °C) for a duration of 0, 4, 8 and 12 days to evaluate their effects on fruit quality. The data collected was separately subjected to a two-way ANOVA in a completely randomised design using the GenStat statistical software package (14th edition). Storage temperatures and durations significantly (p < 0.05) decreased papaya quality. Mountain fruits were firmer while F fruits had more appealing peel and flesh colors. F fruits retained more potassium (1.20 g/100g) and vitamin C (0.29 g/100g) when stored at 16 °C on day 12. The optimal storage temperature and duration for successful storage and extension of shelf life of papaya fruits was found to be 16 °C for 8 days. The F hybrid fruits were more comparable to the local papaya variety, Mountain, on the various tested parameters, which could make it suitable for consumer utilization and market exploitation in Kenya.