Evaluation of Phytochemical, Antioxidant and Hepatoprotective Activity of Tuber of Geodorum laxiflorum Griff

Besra, Ram Chandra; Majhee, Lakhan; Sharma, J.

doi:10.3923/jpt.2011.610.623

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…Commercial importance of orchids has led to their tremendous production in recent years. Apart from their ornamental value orchids are also known for their medicinal usage especially in the traditional system of medicine (Besra et al 2011). It is believed that the Chinese were the first to cultivate and describe orchids for medicinal purpose.…”

Section: Introductionmentioning

confidence: 99%

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

Pyati¹

2022

Plant Tissue Cult. & Biotech.

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An efficient in vitro regeneration protocol through somatic embryogenesis was established from longitudinally bisected protocorm (lTCL) of an endangered orchid Dendrobium ovatum. The efficiency of EC and SEs from the protocorms significantly relied on the concentration of PGRs. MS medium supplemented with TDZ (1.0 mg/l) induced optimum of EC (31.8%) and SEs (28.1/explant). Similarly, ZEA (0.5 mg/l) induced EC (27.6%) and SEs (17.1/explant). The combined treatment of TDZ (1.0 mg/l) and NAA (0.5 mg/l) resulted in the maximum induction of EC (58.6%) and SEs (39.8/explant) in an upright incubated explant. In another combined effect of ZEA (1.0 mg/l) and NAA (0.5 mg/l) induced EC (43.8%) and SEs (24.3/explant), whereas the combination of BAP (0.5 mg/l) and NAA (0.5 mg/l) produced EC (34.4%) and SEs (16.8/explants). The induced EC and SEs where healthier on medium containing TDZ + NAA than on the medium containing ZEA+NAA and BAP+NAA. The orientation of lTCL explants seemed to interact with position to affect the morphogenesis. The lTCL explants incubated in the upright orientation induced higher percentage of EC and a greater number of SEs/explants (EC-58.6% and SEs-39.8/explants) than that in the inverted orientation (EC- 16.4% and SEs-9.1/explants) irrespective of the PGRs. The SEs developed from the EC and the intermediate stage of PLBs were finally differentiated into plantlets. About 89% of plantlets were successfully acclimatized in the green house conditions. Plant Tissue Cult. & Biotech. 32(1): 53-66, 2022 (June)

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Section: Introductionmentioning

confidence: 99%

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

Pyati¹

2022

Plant Tissue Cult. & Biotech.

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“…They are present through-out the world but there is an exception of Antarctic region and some desert area [2]. The application of various known varieties of orchids in conventional folklore system is customary and they are widely accepted and appreciated as they have high content of alkaloids, glycosides, phenols, and phyto-chemicals [3,4].…”

Section: Introductionmentioning

confidence: 99%

Orchids as Valuable Folklore Medicine: A Review

Sushma

Diksha

2021

JPRI

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The impetus of writing this paper is to review various medicinal orchid species growing in different parts of India. The family orchidaceae is one of the diverse groups among angiosperms which includes different types of orchid species. This paper is written to review the medicinal and therapeutic importance and use of different orchid species for treatment of various types of diseases. Orchids are herbaceous plants which are mentioned in ancient ayurvedic system of medicine because of their therapeutic properties. Most of the medicinal orchid species are epiphytic and terrestrial in habitat.

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“…Paul et al (2013) worked on three species of orchids namely Areides odorata, Acampe papilosa and Acampe ochracea against ampicillin resistant srain of E. coli. Besra et al (2011) was evaluated of antioxidant of tuber Geodorum laxiflorum. More detail Dendrobium, the third largest genus of the Orchidaceae family, has been reported containing compounds that have the potential to be developed as antimicrobials namely D. nutantiflorum (Rashmi et al, 2015), D. crumenatum (Sandrasagaran et al, 2014), D. amoenum, D. crepidatum, D. moniliforme, D. longicomu (Paudel et al, 2018, D. nobile as an antitumor (Devi et al, 2009), antioxidant (Prasad, et al, 2016), antitoxin (Shrestha et al, 2015.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Screening, Antibacterial and Cytotoxic Activity of Dendobium lasianthera J.J.Sm. from Papua

et al. 2020

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The aims of the study are to evaluate bioactive compounds, antibacterial and cytotoxic potential of D. lasianthera. This orchid grows well all over New Guinea Island as an ornamental plant because of their beautiful flowers. Orchids also known rich of its phytochemical compounds which already used as a traditional medicine in many countries around the world. However, research in pharmacological fields is still limited. In this study, leaves and stem of D. lasianthera were powdered and extracted with ethanol followed by fractionated using n-hexane, ethyl acetate, and ethanol solvent. Extract as well as fraction were tested for phytochemical screening and determined antibacterial activity against Escherichia coli and Staphylococcus aureus using Disc Diffusion Method. Brine Shrimp Lethality Test (BSLT) was used to observe cytotoxic potential of leaves and stem extract and fraction at 10, 50, 100, 250, 500, 750, and 1000 ppm. The results showed that the leaves and stem extract contained tannin and alkaloids, separately. The ethanol extract of D. lasianthera showed 7.35 mm (leaves) and 7.43 mm (stem) inhibition zone against S. aureus Furthermore, the maximum inhibition zone of ethanol fraction of leaves and n-hexane fraction of stem were 8.42 mm and 8.10 mm separately. The LC50 of stem extract and fractions in these study were 699.3 ppm (ethanol extract), 602.1 ppm (ethyl acetate), 329.6 ppm (n-hexane fraction) and 676 ppm (ethanol fraction), whereas for leaves, only ethyl acetate fraction has toxict activity with an average LC50 833.2 ppm. Key words: D. lasianthera; phytochemical screening; antibacterial; cytotoxicity; Papua.

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Evaluation of Phytochemical, Antioxidant and Hepatoprotective Activity of Tuber of Geodorum laxiflorum Griff

Cited by 4 publications

References 30 publications

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

Orchids as Valuable Folklore Medicine: A Review

Phytochemical Screening, Antibacterial and Cytotoxic Activity of Dendobium lasianthera J.J.Sm. from Papua

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