Evaluation of plant cell wall degrading enzyme production by Clostridium thermocellum B8 in the presence of raw agricultural wastes

Hamann, Pedro Ricardo Vieira; Serpa, Dayane L.; Cunha, Amanda Souza Barreto da; Camargo, Brenda Rabello de; Osiro, Karen Ofuji; Sousa, Marcelo Valle de; Félix, Carlos Roberto; Miller, Robert N.; Noronha, Eliane Ferreira

doi:10.1016/j.ibiod.2015.08.013

Cited by 17 publications

(5 citation statements)

References 39 publications

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“…The utilization of plant biomass for feed productions is limited by their structure and composition, and the structural modification and removal of lignin are required to enhance the enzymatic accessibility of polysaccharides in the biomass before enzymatic saccharification (Hamann et al 2015…”

Section: Discussionmentioning

confidence: 99%

Improvement of fermentation quality and cellulose convertibility of Napier grass silage by inoculation of cellulolytic bacteria from Tibetan yak ( Bos grunniens )

Tang

Zhao

et al. 2020

J Appl Microbiol

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Aims To isolate and identify cellulolytic bacteria from yak rumen and further evaluate the effects of the isolates on the silage quality, structural carbohydrates degradation and cellulose convertibility of Napier grass silage. Methods and Results Two out of 218 strains were selected based on their most extensive transparent zone and the highest filter paper disintegration rate. The two isolates (JFL12 and JF85) could grow normally at 15–55°C, pH 3·0–7·0 and NaCl (3·0, 6·5%), and were identified as Enterococcus casseliflavus and Enterococcus faecalis by 16S rDNA sequence analysis, respectively. Napier grass was ensiled with no additive control (C), Lactobacillus plantarum (Lp), JFL12, JF85, JFL12 + Lp and JF85 + Lp for 3, 5, 7, 14, 30 and 60 days. All inoculated silages had higher lactic acid content, lower pH, ammonia nitrogen (NH3‐N) and lignocellulose contents than the control silage. Silages treated with JFL12 + Lp and JF85 + Lp had the lowest pH and NH3‐N contents, the highest lactic acid content and lignocellulose degradation among all treatments. The isolates with or without Lp significantly (P < 0·01) increased water soluble carbohydrates (WSC), glucose, fructose and sucrose contents as compared with the control silage. Silages treated with JFL12 + Lp and JF85 + Lp had higher glucose yield and cellulose convertibility than the other silages. Conclusions Therefore, the application of isolates (JFL12 and JF85) with Lp had synergistic effects on accelerating the degradation of structural carbohydrates and improving the silage quality. Significance and Impact of the Study Napier grass presents difficulty to ensiling due to its low WSC and high structural carbohydrates contents. The screened cellulolytic bacteria could be a candidate strain in improving fermentation quality and structural carbohydrates degradability of ensiled forages.

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Section: Discussionmentioning

confidence: 99%

Improvement of fermentation quality and cellulose convertibility of Napier grass silage by inoculation of cellulolytic bacteria from Tibetan yak ( Bos grunniens )

Tang

Zhao

et al. 2020

J Appl Microbiol

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“…It was first isolated from manure in 1926 [163] and further strains are frequently isolated from decaying biomass and soil [17,164], some mesophilic environments [165], as well as thermophilic anaerobic digesters [166,167]. Similarly to C. stercorarium, C. thermocellum has been reclassified numerous times in recent years, first to the Ruminiclostridium genus [77], then the Hungateiclostridium genus [94], and very recently to the genus Acetivibrio, as A.…”

Section: Clostridium Thermocellumbest Candidate For Cbpmentioning

confidence: 99%

Digestibility of Wheat and Cattail Biomass Using a Co-culture of Thermophilic Anaerobes for Consolidated Bioprocessing

et al. 2020

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Alternative low-carbon transportation fuels, such as biofuels, are needed to replace or supplement fossil fuels in order to lower global greenhouse gas emissions and combat climate change. Lignocellulosic biofuels have relatively low carbon emissions and are created using the non-food parts of crops and other plants, such as the leaves and stems, which are comprised mostly of a tough material called lignocellulose, composed of cellulose, hemicellulose, and lignin. One of the best lignocellulose degraders found in nature that is also capable of fermenting the released sugars to ethanol is Clostridium thermocellum, although improvements in both lignocellulose hydrolysis extent and ethanol yields are needed for commercial viability.C. thermocellum, considered a cellulose-degrading specialist, was co-cultured with two different hemicellulose-specialists, C. stercorarium and Thermoanaerobacter thermohydrosulfuricus. The hypothesis was that the co-cultures might degrade more lignocellulose owing to the additional hydrolytic enzymes supplied by the partners and their ability to uptake the inhibitory hemicellulose sugars. All co-culture combinations were found to solubilize more wheat straw, among other lignocellulose materials, and produce more endproducts, including ethanol, than C. thermocellum alone. These co-cultures were stable over multiple serial passages, on either wheat straw or pure cellulose, although some evidence of carbon competition was observed. The tri-culture was successfully used to screen the digestibility of various lignocellulose materials, revealing substantial difference between cattail harvested in different seasons. Cross-feeding of vital growth factors was observed between the various co-culture members in a defined medium.The metabolism of C. thermocellum is atypical compared to many organisms, including the absence of a pyruvate kinase, and its substitution with both a malate shunt and a putative Thank you to my friends and family for always supporting me along this journey. I would like to thank my supervisor, Dr. Richard Sparling, for giving me this opportunity and for his incredibly valuable guidance and mentorship, the strict scientific rigour helped me develop my critical thinking skills.

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“…The decrease in fossil fuel resources and the related environmental pollution are directing the world's energy policies towards the development of alternative energy resources (Liu 2020, Oni et al 2020. In this regard, there is a global interest in developing industrial processes to produce biofuels from plant biomass such as natural oils, starch, sucrose and lignocelluloses (Hamann et al 2015). Lignocellulosic materials, composed of lignin, pectin, hemicellulose, and cellulose(10-40% 5-10%, 20-40%, and 45-60%, respectively), are found as cell wall components in plants (Guzel & Akpinar 2019, Ravindran & Jaiswal 2016.…”

Section: Introductionmentioning

confidence: 99%

Production of Endoglucanase by Exiguobacterium mexicanum OB24 Using Waste Melon Peels as Substrate

Baltaci

Omeroglu

Albayrak

et al. 2022

An. Acad. Bras. Ciênc.

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Millions of tons of agricultural waste are produced globally every year.A practical solution to this global problem is to convert this waste into value-added products. In this study, endoglucanase enzyme production was carried out by using waste melon peels as a carbon source. To use this important resource, its stubborn structure must be broken down. Rumen bacteria are regarded as unique for this job.Therefore, fi rstly endoglucanase producing rumen bacteria was isolated and the bacteria with the best activity (OB24) were identifi ed by molecular methods (16S rRNA gene squencing). As a result of the sequence analysis, it was determined that isolate belonged to Exiguobacterium mexicanum. Then, by optimizing the culture conditions, the enzyme production potential was increased. The optimal conditions were determined as 50 g/L MPP, 2g/L yeast extract, 60 h incubation time, pH: 6.0, and 40°C temperature.Under optimized conditions the enzyme activity increased approximately 3.8-fold.

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Evaluation of plant cell wall degrading enzyme production by Clostridium thermocellum B8 in the presence of raw agricultural wastes

Cited by 17 publications

References 39 publications

Improvement of fermentation quality and cellulose convertibility of Napier grass silage by inoculation of cellulolytic bacteria from Tibetan yak ( Bos grunniens )

Improvement of fermentation quality and cellulose convertibility of Napier grass silage by inoculation of cellulolytic bacteria from Tibetan yak ( Bos grunniens )

Digestibility of Wheat and Cattail Biomass Using a Co-culture of Thermophilic Anaerobes for Consolidated Bioprocessing

Production of Endoglucanase by Exiguobacterium mexicanum OB24 Using Waste Melon Peels as Substrate

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