Evaluation of Poly lactide-co-glycolide Acid [PLGA] Nanoparticles Loaded with Praziquantel in treatment of experimental murine Schistosomiasis mansoni.

Abo-Sheishaa, Gamal A.

doi:10.21608/evmspj.2019.145815

Cited by 1 publication

(3 citation statements)

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“…Our study uses SPL-loaded PLGA NPs which showed better results than free drug. Our results were in agreement with AboSheishaa 76 whose study used PLGA NPs loaded with PZQ. The authors found that PLGA NPs improved the antischistosomal effects of PZQ with highly significant reduction in mean total worm burden, the highest elevation of the percentage of dead ova (95.31%), with complete disappearance of immature ova and the highest reduction of egg load in hepatic tissue by 97.1%.…”

Section: Electron Microscope Studiessupporting

confidence: 92%

“…The authors found that PLGA NPs improved the antischistosomal effects of PZQ with highly significant reduction in mean total worm burden, the highest elevation of the percentage of dead ova (95.31%), with complete disappearance of immature ova and the highest reduction of egg load in hepatic tissue by 97.1%. 76 Another team work 77 potentiate the antischistosomal abilities of the synthetic preparation (the −6,60-dinitrohinokinin (DNHK)) by incorporation to PLGA nanoparticles. The in vitro incubation of adult worms was associated with marked reduction in motor activity and egg production.…”

Section: Electron Microscope Studiesmentioning

confidence: 99%

“…The authors found that PLGA NPs improved the antischistosomal effects of PZQ with highly significant reduction in mean total worm burden, the highest elevation of the percentage of dead ova (95.31%), with complete disappearance of immature ova and the highest reduction of egg load in hepatic tissue by 97.1%. 76 Another team work 77 potentiate the antischistosomal abilities of the synthetic preparation (the 78 incorporated curcumin into PLGA nanoparticles and tested its ability in vitro on Schistosoma worms. They declared that curcumin-loaded PLGA nanoparticles caused partial alterations in the tegument of the adult worms in concentrations nearly 40 μM, revealed by the presence of vesicles in its structure, while that action was produced by the curcumin free drug only after 100 μM.…”

Section: Electron Microscope Studiesmentioning

confidence: 99%

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The Idiosyncratic Efficacy of Spironolactone-Loaded PLGA Nanoparticles Against Murine Intestinal Schistosomiasis

Hady¹,

El-Emam²,

Saleh³

et al. 2023

IJN

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Background Schistosomiasis is a chronic debilitating parasitic disease accompanied with severe mortality rates. Although praziquantel (PZQ) acts as the sole drug for the management of this disease, it has many limitations that restrict the use of this treatment approach. Repurposing of spironolactone (SPL) and nanomedicine represents a promising approach to improve anti-schistosomal therapy. We have developed SPL-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) to enhance the solubility, efficacy, and drug delivery and hence decrease the frequency of administration, which is of great clinical value. Methods The physico-chemical assessment was performed starting with particle size analysis and confirmed using TEM, FT-IR, DSC, and XRD. The antischistosomal effect of the SPL-loaded PLGA NPs against Schistosoma mansoni ( S. mansoni )-induced infection in mice was also estimated. Results Our results manifested that the optimized prepared NPs had particle size of 238.00 ± 7.21 nm, and the zeta potential was −19.66 ± 0.98 nm, effective encapsulation 90.43±8.81%. Other physico-chemical features emphasized that nanoparticles were completely encapsulated inside the polymer matrix. The in vitro dissolution studies revealed that SPL-loaded PLGA NPs showed sustained biphasic release pattern and followed Korsmeyer–Peppas kinetics corresponding to Fickian diffusion ( n <0.45). The used regimen was efficient against S. mansoni infection and induced significant reduction in spleen, liver indices, and total worm count ( ρ <0.05). Besides, when targeting the adult stages, it induced decline in the hepatic egg load and the small intestinal egg load by 57.75% and 54.17%, respectively, when compared to the control group. SPL-loaded PLGA NPs caused extensive damage to adult worms on tegument and suckers, leading to the death of the parasites in less time, plus marked improvement in liver pathology. Conclusion Collectively, these findings provided proof-of-evidence that the developed SPL-loaded PLGA NPs could be potentially used as a promising candidate for new antischistosomal drug development.

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Section: Electron Microscope Studiessupporting

confidence: 92%

Section: Electron Microscope Studiesmentioning

confidence: 99%

“…The authors found that PLGA NPs improved the antischistosomal effects of PZQ with highly significant reduction in mean total worm burden, the highest elevation of the percentage of dead ova (95.31%), with complete disappearance of immature ova and the highest reduction of egg load in hepatic tissue by 97.1%. 76 Another team work 77 potentiate the antischistosomal abilities of the synthetic preparation (the 78 incorporated curcumin into PLGA nanoparticles and tested its ability in vitro on Schistosoma worms. They declared that curcumin-loaded PLGA nanoparticles caused partial alterations in the tegument of the adult worms in concentrations nearly 40 μM, revealed by the presence of vesicles in its structure, while that action was produced by the curcumin free drug only after 100 μM.…”

Section: Electron Microscope Studiesmentioning

confidence: 99%

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