Evaluation of poly(lactide-co-glycolide)/hydroxyapatite nanofibres for reconstruction of critical-sized segmental bone defects in a canine model

Heo, Sy; Kim, H.Y.; Kim, N.S.

doi:10.17221/283/2015-vetmed

Cited by 3 publications

(4 citation statements)

References 23 publications

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“…The nanofibrous structures mimic the natural extracellular matrix and provide a starting place for the cell attachment, proliferation, and differentiation (Bhardwaj and Kundu 2010). Our nanofibrous structures were made by using electrospinning to mimic the bone marrow and were instilled with gelatine because it is suitable for cell adhesion on the electrospun nanofibre (Heo et al 2017). However, the bone defects transplanted with the PLGA/HAp nanofibrous scaffolds in our https://doi.org/10.17221/80/2019-VETMED study were not sufficiently filled with new bone tissue in Group 2.…”

Section: Discussionmentioning

confidence: 99%

“…21223, Sigma-Aldrich, Gillingham, UK). The electrospun PLGA/HAp was manufactured according to the method previously described by Heo et al (2017). Dissolution of the PLGA was performed by utilising methylene chloride (MC) and dimethylformamide (DMF) (Du Pont, Michigan, USA) at an 80/20 ratio to obtain a 10 wt% solution.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, cell attachment, proliferation, and mineralisation can occur because of its wide surface area (Mazaheri et al 2015). Numerous in vitro studies have demonstrated the possibilities of using poly(lactic-acid) and poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HAp) nanofibres for bone tissue engineering, which are fabricated using 3D printing with FDM technology and electrospinning (Lipner et al 2014;Haider et al 2015;Stachewicz et al 2015;Heo et al 2017). However, the authors of these in vitro reports were unable to assess the biomechanical properties of the engineered tissue.…”

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confidence: 99%

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Evaluation of a poly(lactic-acid) scaffold filled with poly(lactide-co-glycolide)/hydroxyapatite nanofibres for reconstruction of a segmental bone defect in a canine model

Yun¹,

Heo²,

Lee³

et al. 2019

Vet. Med. - Czech

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Critical-sized bone defects are a difficult problem in both human and veterinary medicine. To address this issue, synthetic graft materials have been garnering attention. Abundant in vitro studies have proven the possibilities of poly(lactic-acid) (PLA) scaffolds and poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HAp) nanofibres for treating bone defects. The present study aimed at conducting an in vivo assessment of the biological performance of a three dimensional (3D)-printed PLA scaffold filled with a PLGA/HAp nanofibrous scaffold to estimate its potential applications in bone defect reconstruction surgery. Defects were created in a 20 mm-long region of the radius bone. The defects created on the right side in six Beagle dogs (n = 6) were left untreated (Group 1). The defects on the left side (n = 6) were filled with 3D-printed PLA scaffolds incorporated with PLGA/Hap nanofibres with gelatine (Group 2). The other six Beagle dog defects were made bilaterally (n = 12) and filled with the same material as that used in Group 2 along with recombinant bone morphogenetic protein 2 (rhBMP-2) (Group 3). Both the radiological and histological examinations were performed for observing the reaction of the scaffold and the bone. Micro-computed tomography (CT) was utilised for the evaluation of the bone parameters 20 weeks after the experiment. The radiological and histological results revealed that the scaffold was biodegradable and was replaced by new bone tissue. The micro-CT revealed that the bone parameters were significantly (P < 0.05) increased in Group 3. Based on these results, our study serves as a foundation for future studies on bone defect treatment using synthetic polymeric scaffolds.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Evaluation of a poly(lactic-acid) scaffold filled with poly(lactide-co-glycolide)/hydroxyapatite nanofibres for reconstruction of a segmental bone defect in a canine model

Yun¹,

Heo²,

Lee³

et al. 2019

Vet. Med. - Czech

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“…The manufacturing of nanofibers by electrospinning provided the possibility to fabricate scaffolds used for tissue reconstruction and regeneration. For example, biodegradable poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HAp) electrospun nanofibers were used as bone scaffold biomaterial to repair critical-sized segmental bone defects in a canine model [ 89 ]. Nanofibers have also been applied as scaffolds to support tissue-specific cell functions and tissue-mimicking systems for tissue/organ regeneration [ 90 ].…”

Section: Current Applications Of Nanomaterials In Veterinary Medicinementioning

confidence: 99%

Meeting Contemporary Challenges: Development of Nanomaterials for Veterinary Medicine

Danchuk,

Levchenko,

da Silva Mesquita

et al. 2023

Pharmaceutics

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In recent decades, nanotechnology has been rapidly advancing in various fields of human activity, including veterinary medicine. The review presents up-to-date information on recent advancements in nanotechnology in the field and an overview of the types of nanoparticles used in veterinary medicine and animal husbandry, their characteristics, and their areas of application. Currently, a wide range of nanomaterials has been implemented into veterinary practice, including pharmaceuticals, diagnostic devices, feed additives, and vaccines. The application of nanoformulations gave rise to innovative strategies in the treatment of animal diseases. For example, antibiotics delivered on nanoplatforms demonstrated higher efficacy and lower toxicity and dosage requirements when compared to conventional pharmaceuticals, providing a possibility to solve antibiotic resistance issues. Nanoparticle-based drugs showed promising results in the treatment of animal parasitoses and neoplastic diseases. However, the latter area is currently more developed in human medicine. Owing to the size compatibility, nanomaterials have been applied as gene delivery vectors in veterinary gene therapy. Veterinary medicine is at the forefront of the development of innovative nanovaccines inducing both humoral and cellular immune responses. The paper provides a brief overview of current topics in nanomaterial safety, potential risks associated with the use of nanomaterials, and relevant regulatory aspects.

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Recent Advances in Synthetic and Natural Biomaterials‐Based Therapy for Bone Defects

Echazú

Perna

Olivetti

et al. 2022

Macromolecular Bioscience

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Synthetic and natural biomaterials are a promising alternative for the treatment of critical-sized bone defects. Several parameters such as their porosity, surface, and mechanical properties are extensively pointed out as key points to recapitulate the bone microenvironment. Many biomaterials with this pursuit are employed to provide a matrix, which can supply the specific environment and architecture for an adequate bone growth. Nevertheless, some queries remain unanswered. This review discusses the recent advances achieved by some synthetic and natural biomaterials to mimic the native structure of bone and the manufacturing technology applied to obtain biomaterial candidates. The focus of this review is placed in the recent advances in the development of biomaterial-based therapy for bone defects in different types of bone. In this context, this review gives an overview of the potentialities of synthetic and natural biomaterials: polyurethanes, polyesters, hyaluronic acid, collagen, titanium, and silica as successful candidates for the treatment of bone defects.

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Evaluation of poly(lactide-co-glycolide)/hydroxyapatite nanofibres for reconstruction of critical-sized segmental bone defects in a canine model

Cited by 3 publications

References 23 publications

Evaluation of a poly(lactic-acid) scaffold filled with poly(lactide-co-glycolide)/hydroxyapatite nanofibres for reconstruction of a segmental bone defect in a canine model

Evaluation of a poly(lactic-acid) scaffold filled with poly(lactide-co-glycolide)/hydroxyapatite nanofibres for reconstruction of a segmental bone defect in a canine model

Meeting Contemporary Challenges: Development of Nanomaterials for Veterinary Medicine

Recent Advances in Synthetic and Natural Biomaterials‐Based Therapy for Bone Defects

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