2019
DOI: 10.1016/j.fm.2019.103254
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Evaluation of porcine gastric mucin assay for detection and quantification of human norovirus in fresh herbs and leafy vegetables

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Cited by 16 publications
(12 citation statements)
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“…This new method will allow future survival and inactivation studies on LMFs to be harnessed to infectivity assays. We extended the PGM-MB method to capture FCV and MNV from corn flakes, pistachios, and chocolate using the protocol that has been described previously (51).…”
Section: Resultsmentioning
confidence: 99%
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“…This new method will allow future survival and inactivation studies on LMFs to be harnessed to infectivity assays. We extended the PGM-MB method to capture FCV and MNV from corn flakes, pistachios, and chocolate using the protocol that has been described previously (51).…”
Section: Resultsmentioning
confidence: 99%
“…A 1:10 bead-to-sample ratio was used for all the bead-based extractions (100 μL of beads for 1 mL of eluate). PGM-MBs were prepared as described previously (15,51). To recover FCV or MNV from inoculated chocolate using the bead-based method, the surface of the chocolate was swabbed with a premoistened cotton swab five times, and the virus was released in 800 μL of PBS and extracted using 80 μL of PGM-MBs.…”
Section: Methodsmentioning
confidence: 99%
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“…A 1:10 bead to sample ratio was used for all the bead-based extractions (100 µl of beads for 1ml of eluate). Porcine gastric-coated magnetic beads (PGM-MB) were prepared as described previously (11, 22). To recover FCV or MNV from inoculated chocolate using the bead-based method, the surface of the chocolate was swabbed with a pre-moistened cotton swab five times and the virus was released in 800 µl PBS and extracted using 80 µl PGM-MB beads.…”
Section: Methodsmentioning
confidence: 99%
“…In our previous study, we have developed a magnetic bead-based assay for the isolation of HAV and norovirus surrogates, feline calicivirus (FCV) and murine norovirus (MNV), from LMFs and compared its efficacy against the adapted ISO 15216-1 method (10). We have demonstrated that while both methods can be employed to recover viruses from LMFs, each method has its own advantages and disadvantages (10, 11). Thus, both methods were applied in the present study to investigate viral persistence and infectivity during four-week storage in LMFs.…”
Section: Introductionmentioning
confidence: 99%