Evaluation of post-thaw DNA integrity of mouse blastocysts after ultrarapid and slow freezing

Kader, A.; Agarwal, Ashok; Abdelrazik, H.; Sharma, Rakesh K.; Ahmady, Ali; Falcone, Tommaso

doi:10.1016/j.fertnstert.2008.04.049

Cited by 48 publications

(57 citation statements)

References 44 publications

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“…We have previously demonstrated that both slow cryopreservation and vitrification can induce DNA damage to the blastocysts [23]. We have also demonstrated along with other investigators that having an opening in the zona pellucida may improve the vitrification outcome [24,25].…”

Section: Introductionsupporting

confidence: 62%

“…As previously described [23], blastocysts were vitrified with incubation in the 1st equilibration media for 4 min then vitrification media for 60 s. Two to three blastocysts were then loaded in cryotips which were immediately heat sealed and transferred into liquid nitrogen.…”

Section: Vitrification and Warmingmentioning

confidence: 99%

“…Warmed blastocysts were finally incubated in HTF medium in 5% CO 2 for 4 h before further processing [23].…”

Section: Vitrification and Warmingmentioning

confidence: 99%

“…Slow cryopreservation was done using glycerol based blastocyst freeze media (Irvine scientific, Santa Ana, CA) as previously described [23]. Two to three blastocysts were loaded into conventional 0.25 mL cryopreservation straws (IMV-Technologis-L'aigle, France).…”

Section: Slow Freezingmentioning

confidence: 99%

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Slow and ultrarapid cryopreservation of biopsied mouse blastocysts and its effect on DNA integrity index

Kader

Falcone

Sharma

et al. 2010

J Assist Reprod Genet

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Section: Introductionsupporting

confidence: 62%

Section: Vitrification and Warmingmentioning

confidence: 99%

“…Warmed blastocysts were finally incubated in HTF medium in 5% CO 2 for 4 h before further processing [23].…”

Section: Vitrification and Warmingmentioning

confidence: 99%

Section: Slow Freezingmentioning

confidence: 99%

See 2 more Smart Citations

Slow and ultrarapid cryopreservation of biopsied mouse blastocysts and its effect on DNA integrity index

Kader

Falcone

Sharma

et al. 2010

J Assist Reprod Genet

Self Cite

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“…However, cryopreservation of expanded blastocysts, with slow freezing or vitrification, or by prolonged incubation of blastocysts in cryoprotectants before vitrification, significantly reduces DNA integrity (Kader et al, 2009(Kader et al, , 2010. On the contrary, vitrifying nonexpanded blastocysts or artificial blastocoel shrunk expanded blastocysts does not result in any significant increase in the number of TUNEL-positive cells (Kader et al, 2010).…”

Section: Dna Integritymentioning

confidence: 99%

Cryopreservation of mammalian embryos: Advancement of putting life on hold

Tsang

Chow

2010

Birth Defects Research Pt C

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Rodent transgenesis and human-assisted reproductive programs involve multistep handling of preimplantation embryos. The efficacy of production and quality of results from conventionally scheduled programs are limited by temporal constraints other than the quality and quantities of embryos per se. The emergence of vitrification, a water ice-free cryopreservation technique, as a reliable way to arrest further growth of preimplantation embryos, provides an option to eliminate the time constraint. In this article, current and potential applications of cryopreservation to facilitate laboratory animal experiments, colony management, and human-assisted reproductive programs are reviewed. Carrier devices developed for vitrification in the last two decades are compared with an emphasis on their physical properties that infer cooling rate of samples and sterility assurance. Biological impacts of improved cryopreservation on preimplantation embryos are also discussed.

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Recryopreservation impairs blastocyst implantation potential via activated endoplasmic reticulum stress pathway and induced apoptosis

Wang,

Zhou,

Long

et al. 2024

MedComm

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Recryopreservation (recryo) is occasionally applied in clinical, while the underlying mechanism of impaired clinical outcomes after recryo remains unclear. In this study, frozen embryo transfer (FET) cycles of single blastocyst transfer in an academic reproductive medicine center were enrolled. According to the number of times blastocysts experienced cryopreservation, they were divided into the cryopreservation (Cryo) group and the Recryo group. Donated human blastocysts were collected and detected for mechanism exploration. It was found that recryo procedure resulted in impaired blastocyst developmental potential, including decreased implantation rate, reduced biochemical pregnancy rate, declined clinical pregnancy rate, higher early miscarriage rate, and lower live birth rate. Moreover, recryo led to impaired trophectoderm (TE) function, exhibiting lower human chorionic gonadotropin levels 12 days after FET. In addition, single‐cell RNA sequencing showed that the expression of genes involved in cell adhesion and embryo development were altered. More specifically, activated endoplasmic reticulum (ER) pathway and induced apoptosis were further verified by immunofluorescence and terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling (TUNEL) assay involving in the recryo procedure. In conclusion, recryo could interfere with the process of blastocyst implantation by impairing TE function, affecting blastocyst adhesion, activating ER stress pathway and inducing apoptosis. It provides caution to embryologists about the potential risk of recryopreservation.

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Evaluation of post-thaw DNA integrity of mouse blastocysts after ultrarapid and slow freezing

Cited by 48 publications

References 44 publications

Slow and ultrarapid cryopreservation of biopsied mouse blastocysts and its effect on DNA integrity index

Slow and ultrarapid cryopreservation of biopsied mouse blastocysts and its effect on DNA integrity index

Cryopreservation of mammalian embryos: Advancement of putting life on hold

Recryopreservation impairs blastocyst implantation potential via activated endoplasmic reticulum stress pathway and induced apoptosis

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