Evaluation of potential genotoxicity of five food dyes using the somatic mutation and recombination test

Sarıkaya, Rabia; Selvı, Mahmut; Erkoç, Fıgen

doi:10.1016/j.chemosphere.2012.03.032

Cited by 87 publications

(40 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With regard to non-azo dyes, Sarıkaya et al (2012) investigated the activity of Bordeaux Red on larvae of Drosophila melanogaster, and found that this additive caused somatic mutations in salivary gland cells of this test organism. Mpountoukas et al (2010) conducted an experiment in human peripheral blood cell cultures to assess the cytotoxic effect of Bordeaux Red, and found that these additives caused significant reduction of the mitotic index and induced the appearance of micronuclei in cells of this test system.…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic and genotoxic potential of powdered juices

et al. 2016

View full text Add to dashboard Cite

Powdered juices are widely consumed by the population especially because of their convenient preparation, availability in various fruit flavors and low cost when compared to other industrialized beverages. They have complex formulation, consisting of several classes of food additives. However, there are no scientific studies on the toxicity of these foods. Thus, this study evaluated the toxicity at the cellular level of industrialized powdered juices of orange and guava flavors of three different food companies. This analysis was made using root meristem cells of Allium cepa L., at the exposure times of 24 and 48 hours, and two concentrations, 30 g/1000 mL, considered ideal for consumption according to the label of the products, and 30 g/500 mL. Both flavors of juices, of the three companies, in both concentrations and the two exposure times promoted significant antiproliferative effect to root meristem cells and caused a statistically significant number of mitotic spindle changes and micronuclei in cells of the test system used. Therefore, under the studied conditions, all the samples of juice powder exhibited cytotoxic and genotoxic potential.

show abstract

Section: Resultsmentioning

confidence: 99%

Cytotoxic and genotoxic potential of powdered juices

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In a study carried out by Sarikaya, Selvi and Erkoç (2012), investigating the effect of the dye bordeaux red in Table 2. Number of micronucleated cells with bridges in anaphase and telophase and total number of aberrant cells treated with 0.4 and 4.0 mL of the food dyes sunset yellow, bordeaux red, and tartrazine yellow at exposure times of 24 and 48 hours.…”

Section: Resultsmentioning

confidence: 99%

Citotoxicity of food dyes sunset yellow (E-110), bordeaux red (E-123), and tatrazine yellow (E-102) on Allium cepa L. root meristematic cells

et al. 2013

View full text Add to dashboard Cite

The objective of this study was to evaluate the cytotoxic effect of the food dyes sunset yellow, bordeaux red, and tartrazine yellow on the cellular cycle of Allium cepa L. Each dye was evaluated at the doses of 0.4 and 4.0 mL, at the exposure times of 24 and 48 hours in root tip cells of Allium cepa L. Slides were prepared and cells were analyzed during the whole cell cycle for cellular aberrations totaling 5,000 total cells for each dose evaluated. The mitotic index was calculated, and statistical analysis was performed using the Chi-squared test (p < 0.05). The results showed that the three dyes used under the evaluated doses and exposure times were cytotoxic to the cells of the system-test used. Further cytotoxicity studies should be conducted for additional results and a proper evaluation of the effect of these three dyes on a cellular level.

show abstract

“…Numerous studies concerning the genotoxic effects of synthetic indigo and its dye solutions have been performed (Dixit and Goyal 2013;Bhattacharjee 2014;Bazin et al 2012;Sarıkaya et al 2012) and mutagenic activity of synthetic indigo was revealed using Ames test (Rannug et al 1992). Furthermore, the lethal doses of patent blue and indigocarmin was reported to be 50 mg/ml (Sarıkaya et al 2010).…”

Section: Introductionmentioning

confidence: 99%

The role of natural indigo dye in alleviation of genotoxicity of sodium dithionite as a reducing agent

et al. 2016

View full text Add to dashboard Cite

Indigo blue is a natural dye used for thousands of years by civilizations to dye fabric blue and it is naturally obtained from Isatis tinctoria. I. tinctoria is not only used for extraction of indigo blue color but also used medicinally in Traditional Chinese Medicine because of its active compounds. Sodium dithionite (Na 2 S 2 O 4 ) is used in dye bath for indigo blue extraction, but this reducing agent and its derivatives are major pollutants of textile industry and subsequently have hazardous influences on public health. Herein, the present study was designed to obtain the high yield of natural indigo dye but with low possible toxic effect. In this context, genotoxic effects of particular combinations of natural dye solutions obtained from Isatis tinctoria subsp. tomentolla with Na 2 S 2 O 4 as reducing agent were investigated. Dye solutions were obtained using two different pH levels (pH 9 and 11) and three different concentrations of Na 2 S 2 O 4 (2.5, 5 and 10 mg/ml). In addition to the dye solutions and reducing agent, aqueous extracts of I. tinctoria were assessed for their genotoxicity on human lymphocytes. For in vitro testing of genotoxicity, chromosomal aberrations (CAs), sister chromatid exchanges (SCEs) and mitotic indexes (MI) assays were used. Accordingly, Na 2 S 2 O 4 caused significant increases in CA and SCE as well decrease in MI but the genotoxic effects of sodium dithionite were reduced with natural indigo dye. As a result, aqueous extracts of Isatis leaves removed the toxic effects of sodium dithionite and showed anti-genotoxic effect. For the optimal and desired quality but with less toxic effects of natural dye, 2.5 mg/ml (for wool yarn) and 5 mg/ml (for cotton yarn) of Na 2 S 2 O 4 doses were found to be the best doses for reduction in the dye bath at Ph 9.

show abstract

Evaluation of potential genotoxicity of five food dyes using the somatic mutation and recombination test

Cited by 87 publications

References 42 publications

Cytotoxic and genotoxic potential of powdered juices

Cytotoxic and genotoxic potential of powdered juices

Citotoxicity of food dyes sunset yellow (E-110), bordeaux red (E-123), and tatrazine yellow (E-102) on Allium cepa L. root meristematic cells

The role of natural indigo dye in alleviation of genotoxicity of sodium dithionite as a reducing agent

Contact Info

Product

Resources

About